7R5C

Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-29 (G206C, R207S, D210L, S211V)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, CCC	Isoaspartyl peptidase	polymer	178	19013.8	2	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli	Beta-aspartyl-peptidase,EcAIII,Isoaspartyl dipeptidase
2	BBB, DDD	Isoaspartyl peptidase	polymer	143	14414.2	2	UniProt (P37595) Pfam (PF01112) In PDB	Escherichia coli	Beta-aspartyl-peptidase,EcAIII,Isoaspartyl dipeptidase
3	AAA, CCC	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	CCC	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	DDD	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6		water	water		18.0	107	Chemie (HOH)

Sequence modifications

BBB, DDD: 179 - 321 (UniProt: P37595)

PDB	External Database	Details
Cys 206	Gly 206	engineered mutation
Ser 207	Arg 207	engineered mutation
Leu 210	Asp 210	engineered mutation
Val 211	Ser 211	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	66856.0
Non-Polymers*	Number of molecules	4
	Total formula weight	230.2
All*	Total formula weight	67086.2

*Water molecules are not included.