7R5C

Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-29 (G206C, R207S, D210L, S211V)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-13
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	51.789, 77.864, 148.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.480 - 2.200
Rwork	0.249
R-free	0.30090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.011
RMSD bond angle	1.622
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.480	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.124	0.496
Rmeas	0.140	0.555
Number of reflections	30380	2652
<I/σ(I)>	6.4	1.9
Completeness [%]	97.6	99.2
Redundancy	4.5
CC(1/2)	0.992	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M PCTP buffer pH 7.0, 25 % w/v PEG 1500