7R5C
Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-29 (G206C, R207S, D210L, S211V)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-13 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.789, 77.864, 148.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.480 - 2.200 |
Rwork | 0.249 |
R-free | 0.30090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zal |
RMSD bond length | 0.011 |
RMSD bond angle | 1.622 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.480 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.124 | 0.496 |
Rmeas | 0.140 | 0.555 |
Number of reflections | 30380 | 2652 |
<I/σ(I)> | 6.4 | 1.9 |
Completeness [%] | 97.6 | 99.2 |
Redundancy | 4.5 | |
CC(1/2) | 0.992 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M PCTP buffer pH 7.0, 25 % w/v PEG 1500 |