7R1F

Early transcription elongation state of influenza B polymerase backtracked due to double incoproation of nucleotide analogue T1106

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polymerase acidic protein	polymer	751	85822.8	1	UniProt (Q5V8Z9) Pfam (PF00603) In PDB	Influenza B virus (B/Memphis/13/2003)	RNA-directed RNA polymerase subunit P2
2	B	RNA-directed RNA polymerase catalytic subunit	polymer	772	86207.0	1	UniProt (Q5V8Y6) Pfam (PF00602) In PDB	Influenza B virus (B/Memphis/13/2003)	Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1
3	C	Polymerase basic protein 2	polymer	798	90844.1	1	UniProt (Q5V8X3) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952) In PDB	Influenza B virus (B/Memphis/13/2003)	RNA-directed RNA polymerase subunit P3
4	V	5' vRNA	polymer	14	4557.8	1		Influenza B virus
5	R	3' vRNA	polymer	21	6706.1	1		Influenza B virus
6	M	mRNA	polymer	20	6282.7	1		Influenza B virus
7	B	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	M	P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE	non-polymer		787.4	1	Chemie (GTA)
9		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A: 1 - 726 (UniProt: Q5V8Z9)

PDB	External Database	Details
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 727	-	expression tag
Ser 728	-	expression tag
Gly 729	-	expression tag
Ser 730	-	expression tag
Gly 731	-	expression tag
Glu 732	-	expression tag
Asn 733	-	expression tag
Leu 734	-	expression tag
Tyr 735	-	expression tag
Phe 736	-	expression tag
Gln 737	-	expression tag

B: 1 - 752 (UniProt: Q5V8Y6)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Glu 758	-	expression tag
Asn 759	-	expression tag
Leu 760	-	expression tag
Tyr 761	-	expression tag
Phe 762	-	expression tag
Gln 763	-	expression tag

C: 1 - 770 (UniProt: Q5V8X3)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 771	-	expression tag
Trp 772	-	expression tag
Ser 773	-	expression tag
His 774	-	expression tag
Pro 775	-	expression tag
Gln 776	-	expression tag
Phe 777	-	expression tag
Glu 778	-	expression tag
Lys 779	-	expression tag
Gly 780	-	expression tag
Ser 781	-	expression tag
Gly 782	-	expression tag
Ser 783	-	expression tag
Glu 784	-	expression tag
Asn 785	-	expression tag
Leu 786	-	expression tag
Tyr 787	-	expression tag
Phe 788	-	expression tag
Gln 789	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	280420.5
Non-Polymers*	Number of molecules	4
	Total formula weight	860.4
All*	Total formula weight	281280.9

*Water molecules are not included.