7QVM

Human Oxytocin receptor (OTR) oxytocin Gq chimera (mGoqi) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(o) subunit alpha,cDNA FLJ31446 fis, clone NT2NE2000909, highly similar to Guanine nucleotide-binding protein G(o) subunit alpha 1	polymer	228	25650.4	1	UniProt (A0A1W2PS82) UniProt (B3KP89) UniProt (P09471) Pfam (PF00503)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39086.6	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (L)	Oxytocin	polymer	10	1008.2	1	UniProt (P01178) Pfam (PF00220)	Homo sapiens	Ocytocin
5	E (R)	Oxytocin receptor	polymer	359	39777.3	1	UniProt (P30559) Pfam (PF00001)	Homo sapiens (human)	OT-R
6	F (S)	Antibody fragment scFv16	polymer	251	26679.7	1		Mus musculus (house mouse)

Sequence modifications

A: 1 - 173 (UniProt: A0A1W2PS82)

PDB	External Database	Details
Asp 9	Glu 9	engineered mutation
Lys 10	Arg 10	engineered mutation
Val 13	Leu 13	engineered mutation
Met 18	Ala 18	engineered mutation
Asp 42	Gly 42	conflict
Asn 43	Glu 43	conflict
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Gly 180	-	linker
Gly 181	-	linker

A: 182 - 231 (UniProt: B3KP89)

PDB	External Database	Details
Asp 227	Ala 227	conflict
Asp 230	Gly 230	conflict

A: 242 - 353 (UniProt: P09471)

PDB	External Database	Details
Ala 332	Ile 332	conflict
Ile 335	Val 335	conflict
Leu 344	Ile 344	engineered mutation
Gln 345	Ala 345	engineered mutation
Met 346	Asn 346	conflict
Glu 350	Gly 350	engineered mutation
Tyr 351	Cys 351	engineered mutation
Asn 352	Gly 352	engineered mutation
Val 354	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

L: 1 - 9 (UniProt: P01178)

PDB	External Database	Details
Nh2 10	-	amidation

R: 1 - 359 (UniProt: P30559)

PDB	External Database	Details
Tyr 153	Asp 153	conflict
Thr 218	Ala 218	variant
Lys 359	Glu 359	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	140063.4
All*	Total formula weight	140063.4

*Water molecules are not included.