7QO1

complex of DNA ligase I and FEN1 on PCNA and DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA ligase 1	polymer	760	84248.6	1	UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679) In PDB	Homo sapiens (human)	DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1
2	B, F, G	Proliferating cell nuclear antigen	polymer	264	29088.1	3	UniProt (P12004) Pfam (PF00705) Pfam (PF02747) In PDB	Homo sapiens (human)	PCNA,Cyclin
3	H	Oligo19ddC	polymer	19	5802.7	1		synthetic construct
4	I	Oligo13P	polymer	13	3976.6	1		synthetic construct
5	J	Oligo32	polymer	32	9845.3	1		synthetic construct
6	Y	Flap endonuclease 1	polymer	380	42617.0	1	UniProt (P39748) Pfam (PF00752) Pfam (PF00867) In PDB	Homo sapiens (human)	FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,hFEN-1
7	I	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)

Sequence modifications

A: 161 - 919 (UniProt: P18858)

PDB	External Database	Details
Cys 160	-	expression tag

B, F, G: 1 - 261 (UniProt: P12004)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Y: 1 - 380 (UniProt: P39748)

PDB	External Database	Details
Ala 181	Asp 181	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	233754.5
Non-Polymers*	Number of molecules	1
	Total formula weight	347.2
All*	Total formula weight	234101.7

*Water molecules are not included.