7QNE

Cryo-EM structure of human full-length synaptic alpha1beta3gamma2 GABA(A)R in complex with Ro15-4513 and megabody Mb38

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	GABA(A) receptor subunit alpha-1	polymer	456	51865.7	2	UniProt (A0A1B0GV38) Pfam (PF02931) Pfam (PF02932) UniProt (by SIFTS) (P14867)	Homo sapiens (human)	Gamma-aminobutyric acid receptor subunit alpha-1
2	B, E (B, E)	Gamma-aminobutyric acid receptor subunit beta-3	polymer	473	54180.3	2	UniProt (P28472) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-3
3	C (C)	GABA(A) receptor subunit gamma-2	polymer	495	56922.1	1	UniProt (A0A286YFI6) Pfam (PF02931) Pfam (PF02932) UniProt (by SIFTS) (P18507)	Homo sapiens (human)	Gamma-aminobutyric acid receptor subunit gamma-2
4	F (G)	Megabody Mb38	polymer	123	13462.9	1		Lama glama
5	G (a)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1559.4	1	In PDB GlyTouCan (G68668TB)
6	H, K (b, e)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
7	I, L (F, H)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	2	In PDB GlyTouCan (G09724ZC)
8	J (d)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
9	M, W (A, D)	[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate	non-polymer		746.6	2	Chemie (PIO)
10	N (A)	N-OCTANE	non-polymer		114.2	1	Chemie (OCT)
11	O, Q, S, X (A, B, C, E)	DECANE	non-polymer		142.3	4	Chemie (D10)
12	P, R, U (A, C, D)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
13	T (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
14	V (D)	ethyl 8-[(azanylidene-$l^{4}-azanylidene)amino]-5-methyl-6-oxidanylidene-4~{H}-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate	non-polymer		326.3	1	Chemie (EIE)

Sequence modifications

C: -38 - 436 (UniProt: A0A286YFI6)

PDB	External Database	Details
-	Pro 376	deletion
-	Ile 377	deletion
-	His 378	deletion
-	His 379	deletion
-	His 380	deletion
-	Ile 381	deletion
-	Gly 382	deletion
-	Asp 383	deletion
-	Ile 384	deletion
-	Val 385	deletion
-	Glu 386	deletion
-	Lys 387	deletion
-	Gln 388	deletion
-	Pro 389	deletion
-	Arg 390	deletion
-	Ile 391	deletion
-	Ser 392	deletion
-	Arg 393	deletion
-	Glu 394	deletion
-	Gln 395	deletion
-	Leu 396	deletion
-	Thr 397	deletion
-	Asp 398	deletion
Leu 338	Ser 400	conflict
Leu 339	Pro 401	conflict
Arg 340	Ser 402	conflict
Met 341	Leu 403	conflict
Phe 342	Pro 404	conflict
Phe 344	Ser 406	conflict
Lys 345	Gln 407	conflict
Gly 437	-	expression tag
Gly 438	-	expression tag
Ser 439	-	expression tag
Gly 440	-	expression tag
Gly 441	-	expression tag
Ser 442	-	expression tag
Gly 443	-	expression tag
Gly 444	-	expression tag
Ser 445	-	expression tag
Gly 446	-	expression tag
Lys 447	-	expression tag
Thr 448	-	expression tag
Glu 449	-	expression tag
Thr 450	-	expression tag
Ser 451	-	expression tag
Gln 452	-	expression tag
Val 453	-	expression tag
Ala 454	-	expression tag
Pro 455	-	expression tag
Ala 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	282477.0
Branched	Number of molecules	6
	Total formula weight	5464.9
Non-Polymers*	Number of molecules	12
	Total formula weight	2830.4
All*	Total formula weight	290772.3

*Water molecules are not included.