7QI1
Crystal structure of human 14-3-3 protein beta in complex with CFTR peptide pS753pS768 and PPI stabilizer CY007424
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, E, F (A, B, C, D) | 14-3-3 protein theta | polymer | 237 | 27115.6 | 4 | UniProt (P27348) Pfam (PF00244) UniProt (by SIFTS) (S7N159) | Homo sapiens (human) | 14-3-3 protein T-cell,14-3-3 protein tau,Protein HS1 |
| 2 | C, D (F, E) | Cystic fibrosis transmembrane conductance regulator | polymer | 28 | 3256.7 | 2 | UniProt (P13569) | Homo sapiens | CFTR,ATP-binding cassette sub-family C member 7,Channel conductance-controlling ATPase,cAMP-dependent chloride channel |
| 3 | G, J (A, B) | ARGININE | non-polymer | 175.2 | 2 | Chemie (ARG) | synthetic construct | ||
| 4 | H, I (A) | [2-(2-methylphenyl)sulfanylphenyl]methanamine | non-polymer | 229.3 | 2 | Chemie (Q95) | synthetic construct | ||
| 5 | K, N (F, C) | TYROSINE | non-polymer | 181.2 | 2 | Chemie (TYR) | synthetic construct | ||
| 6 | L, M (C) | GLUTAMINE | non-polymer | 146.1 | 2 | Chemie (GLN) | synthetic construct | ||
| 7 | O, P, Q, R, S... (A, B, F, E, C...) | water | water | 18.0 | 539 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 3 - 232 (UniProt: P27348)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Met -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Met 1 | - | expression tag |
| Thr 2 | - | expression tag |
| Asp 4 | Glu 2 | conflict |
| Ser 6 | Thr 4 | conflict |
| Val 9 | Ile 7 | conflict |
| Ala 26 | Thr 24 | conflict |
| Ala 27 | Cys 25 | conflict |
| His 36 | Ala 34 | conflict |
| Ala 56 | Gly 54 | conflict |
| Ser 60 | Ala 58 | conflict |
| Glu 72 | Asp 70 | conflict |
| Arg 73 | Thr 71 | conflict |
| Asn 74 | Ser 72 | conflict |
| Glu 75 | Asp 73 | conflict |
| Gln 78 | Leu 76 | conflict |
| Met 80 | Leu 78 | conflict |
| Gly 81 | Ile 79 | conflict |
| Glu 83 | Asp 81 | conflict |
| Ile 88 | Val 86 | conflict |
| Ala 90 | Ser 88 | conflict |
| Gln 93 | Arg 91 | conflict |
| Asp 94 | Ser 92 | conflict |
| Asn 97 | Thr 95 | conflict |
| Asp 98 | Thr 96 | conflict |
| Pro 109 | Ala 107 | conflict |
| Gln 113 | Asn 111 | conflict |
| Ser 132 | Ala 130 | conflict |
| Ser 136 | Cys 134 | conflict |
| Asn 139 | Asp 137 | conflict |
| Lys 140 | Arg 138 | conflict |
| Gln 141 | Lys 139 | conflict |
| Thr 142 | Gln 140 | conflict |
| Val 144 | Ile 142 | conflict |
| Ser 145 | Asp 143 | conflict |
| Gln 149 | Gly 147 | conflict |
| Glu 156 | Asp 154 | conflict |
| Ser 186 | Asn 184 | conflict |
| Lys 189 | Leu 187 | conflict |
| Ser 192 | Thr 190 | conflict |
| Glu 211 | Asp 209 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 114975.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1463.8 | |
| All* | Total formula weight | 116439.4 |
