7QHM

Cytochrome bcc-aa3 supercomplex (respiratory supercomplex III2/IV2) from Corynebacterium glutamicum (stigmatellin and azide bound)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, N (A, N)	Cytochrome bc1 complex Rieske iron-sulfur subunit	polymer	408	45232.2	2	UniProt (Q79VE8) Pfam (PF19297) Pfam (PF00355)	Corynebacterium glutamicum ATCC 13032	Cytochrome bc1 reductase complex subunit QcrA,Menaquinol--cytochrome c reductase iron-sulfur subunit,Rieske iron-sulfur protein
2	B, O (B, O)	Cytochrome bc1 complex cytochrome b subunit	polymer	539	59863.7	2	UniProt (Q79VE9) Pfam (PF13631)	Corynebacterium glutamicum ATCC 13032	Cytochrome bc1 reductase complex subunit QcrB,Menaquinol--cytochrome c reductase cytochrome b subunit
3	C, P (C, P)	Cytochrome bc1 complex cytochrome c subunit	polymer	283	29898.8	2	UniProt (Q8NNK5) Pfam (PF00034) Pfam (PF13442)	Corynebacterium glutamicum ATCC 13032	Cytochrome c1,Cytochrome bc1 reductase complex subunit Qcrc,Menaquinol--cytochrome c reductase cytochrome c subunit
4	D, Q (D, Q)	Cytochrome c oxidase subunit 1	polymer	594	66340.2	2	UniProt (Q79VD7) Pfam (PF00115)	Corynebacterium glutamicum ATCC 13032	Cytochrome aa3 subunit 1,Cytochrome c oxidase polypeptide I
5	E, R (E, R)	Cytochrome c oxidase subunit 2	polymer	331	36811.1	2	UniProt (Q8NNK2) Pfam (PF00116)	Corynebacterium glutamicum ATCC 13032	Cytochrome aa3 subunit 2,Cytochrome c oxidase polypeptide II,Oxidase aa(3) subunit 2
6	F, S (F, S)	Cytochrome c oxidase subunit 3	polymer	205	22457.2	2	UniProt (Q9AEL8) Pfam (PF00510)	Corynebacterium glutamicum ATCC 13032	Cytochrome aa3 subunit 3,Cytochrome c oxidase polypeptide III
7	G, T (G, T)	Cytochrome c oxidase polypeptide 4	polymer	143	15557.3	2	UniProt (Q8NNK3) Pfam (PF12270)	Corynebacterium glutamicum ATCC 13032	Cytochrome aa3 subunit 4,Cytochrome c oxidase polypeptide IV
8	H, U (H, U)	Uncharacterized protein Cgl2664/cg2949	polymer	163	16954.2	2	UniProt (Q8NMB4)	Corynebacterium glutamicum ATCC 13032	P29
9	I, V (I, V)	Uncharacterized membrane protein Cgl2017/cg2211	polymer	147	16385.6	2	UniProt (Q8NP09) Pfam (PF10939)	Corynebacterium glutamicum ATCC 13032	P20
10	J, W (J, W)	Hypothetical membrane protein	polymer	112	12144.2	2	UniProt (Q8NTD4)	Corynebacterium glutamicum ATCC 13032
11	K, X (K, X)	Actinobacterial supercomplex, subunit C (AscC)	polymer	73	8373.3	2	UniProt (Q8NS61)	Corynebacterium glutamicum ATCC 13032
12	L, Y (L, Y)	Hypothetical membrane protein	polymer	65	6628.9	2	UniProt (Q8NSJ8)	Corynebacterium glutamicum ATCC 13032
13	M, Z (M, Z)	Thiamine biosynthesis protein X	polymer	168	17152.4	2	UniProt (P42461)	Corynebacterium glutamicum ATCC 13032
14	AA, TB (A, N)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
15	BA, UB (A, N)	[(2~{R})-3-[[(1~{S},2~{R},3~{S},4~{S},5~{R},6~{R})-2-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxymethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxymethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,4,5-tris(oxidanyl)-6-[(2~{R},3~{S},4~{S},5~{S},6~{R})-3,4,5-tris(oxidanyl)-6-(undecanoyloxymethyl)oxan-2-yl]oxy-cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-2-undecanoyloxy-propyl] (10~{R})-10-methyldodecanoate	non-polymer		1677.8	2	Chemie (IZL)
16	CA, ED, KB, VB (A, U, H, N)	(2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate	non-polymer		853.1	4	Chemie (9YF)
17	AC, DA, IA, RB (O, A, B, N)	MENAQUINONE-9	non-polymer		785.2	4	Chemie (MQ9)
18	AB, AD, BD, EA, EB... (D, T, A, F, P...)	1,2-Distearoyl-sn-glycerophosphoethanolamine	non-polymer		748.1	17	Chemie (3PE)
19	FA, XB (B, O)	STIGMATELLIN A	non-polymer		514.6	2	Chemie (SMA)
20	GA, HA, YB, ZB (B, O)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
21	BC, FB, GB, GD, HB... (O, F, G, V, B...)	CARDIOLIPIN	non-polymer		1464.0	16	Chemie (CDL)
22	CC, LA (O, B)	LYCOPENE	non-polymer		536.9	2	Chemie (LYC)
23	MA, WB (B, O)	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	2	Chemie (LMT)
24	DC, EC, OA, PA (P, C)	HEME C	non-polymer		618.5	4	Chemie (HEC)
25	GC, HC, SA, TA (Q, D)	HEME-AS	non-polymer		921.0	4	Chemie (HAS)
26	IC, UA (Q, D)	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)
27	PC, VA (R, D)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
28	JC, WA (Q, D)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
29	KC, XA (Q, D)	AZIDE ION	non-polymer		42.0	2	Chemie (AZI)
30	BB, CB, QC, RC (E, R)	DINUCLEAR COPPER ION	non-polymer		127.1	4	Chemie (CUA)
31	CD, DB, IB, ID, PB... (U, E, H, Z, M...)	DIACYL GLYCEROL	non-polymer		625.0	6	Chemie (DGA)
32	DD, JB, JD, QB (U, H, Z, M)	PALMITIC ACID	non-polymer		256.4	4	Chemie (PLM)
33	FD, LB (U, H)	[(2~{R})-3-[[(1~{S},2~{R},3~{R},4~{S},5~{S},6~{R})-2-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hexadecanoyloxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-6-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,4,5-tris(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-2-undecanoyloxy-propyl] (10~{S})-10-methylhenicosanoate	non-polymer		1387.7	2	Chemie (IX7)
34	LC (Q)	OXYGEN MOLECULE	non-polymer		32.0	1	Chemie (OXY)
35	AE, BE, KD, LD, MD... (V, X, A, B, C...)	water	water		18.0	416	Chemie (HOH)

Sequence modifications

D, Q: 1 - 584 (UniProt: Q79VD7)

PDB	External Database	Details
Ala 585	-	expression tag
Ala 586	-	expression tag
Trp 587	-	expression tag
Ser 588	-	expression tag
His 589	-	expression tag
Pro 590	-	expression tag
Gln 591	-	expression tag
Phe 592	-	expression tag
Glu 593	-	expression tag
Lys 594	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	26
	Total formula weight	707598.1
Non-Polymers*	Number of molecules	88
	Total formula weight	66643.3
All*	Total formula weight	774241.4

*Water molecules are not included.