7QGK

The mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer in its red state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	The red form of the mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer	polymer	241	27015.9	1	UniProt (Q8ISF8) Pfam (PF01353) In PDB	Entacmaea quadricolor	GFP-like chromoprotein
2	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
3		water	water		18.0	156	Chemie (HOH)

Sequence modifications

A: 9 - 226 (UniProt: Q8ISF8)

PDB	External Database	Details
Gly -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag
Met 2	-	expression tag
Val 3	-	expression tag
Ser 4	-	expression tag
Lys 5	-	expression tag
Gly 6	-	expression tag
Glu 7	-	expression tag
Glu 8	-	expression tag
Lys 17	Met 12	engineered mutation
His 27	Tyr 22	engineered mutation
Glu 36	Asp 31	engineered mutation
Ile 51	Val 46	engineered mutation
Nrp 68	Met 63	chromophore
Nrp 68	Tyr 64	chromophore
Nrp 68	Gly 65	chromophore
Arg 72	Lys 67	engineered mutation
Tyr 77	His 72	engineered mutation
Pro 78	Thr 73	engineered mutation
Val 107	Phe 102	engineered mutation
Val 124	Ala 119	engineered mutation
Gln 125	Lys 120	engineered mutation
Arg 127	Thr 122	engineered mutation
Asp 130	Asn 125	engineered mutation
Pro 136	Ala 131	engineered mutation
Ser 148	Asn 143	engineered mutation
Met 152	Leu 147	engineered mutation
Thr 163	Ser 158	engineered mutation
His 164	Gln 159	engineered mutation
Lys 168	Asn 163	engineered mutation
His 174	Tyr 169	engineered mutation
Val 180	Glu 175	engineered mutation
Gly 190	Glu 185	engineered mutation
Ile 192	Phe 187	engineered mutation
Ile 197	Phe 192	engineered mutation
Ala 199	Phe 194	engineered mutation
Asn 212	Lys 207	engineered mutation
Leu 218	Gln 213	engineered mutation
Arg 219	His 214	engineered mutation
Ser 222	Ala 217	engineered mutation
Ala 227	-	expression tag
Gly 228	-	expression tag
Arg 229	-	expression tag
Gly 230	-	expression tag
Gly 231	-	expression tag
Met 232	-	expression tag
Asp 233	-	expression tag
Glu 234	-	expression tag
Leu 235	-	expression tag
Tyr 236	-	expression tag
Lys 237	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27015.9
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	27088.9

*Water molecules are not included.