7QGK
The mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer in its red state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.338, 66.253, 96.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.620 - 1.500 |
| R-factor | 0.1748 |
| Rwork | 0.174 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u0l |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.500 | 96.500 | 1.530 |
| High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
| Rmerge | 0.025 | 0.021 | 0.209 |
| Rmeas | 0.027 | 0.023 | 0.225 |
| Rpim | 0.010 | 0.010 | 0.083 |
| Total number of observations | 259961 | 1504 | 11478 |
| Number of reflections | 33034 | 242 | 1590 |
| <I/σ(I)> | 40.2 | 116.8 | 4.3 |
| Completeness [%] | 99.8 | 95.6 | 100 |
| Redundancy | 7.9 | 6.2 | 7.2 |
| CC(1/2) | 1.000 | 0.996 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1M bis-tris pH 5.5; 21% PEG 3350 |
