7QG6

Co-crystal structure of UPF3A-RRM-NOPS-L with UPF2-MIF4GIII

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, A, E, G	Regulator of nonsense transcripts 3A	polymer	176	20829.6	4	UniProt (Q9H1J1) Pfam (PF03467) In PDB	Homo sapiens (human)	Nonsense mRNA reducing factor 3A,Up-frameshift suppressor 3 homolog A,hUpf3
2	D, B, F, H	Regulator of nonsense transcripts 2	polymer	321	37791.8	4	UniProt (Q9HAU5) Pfam (PF02854) In PDB	Homo sapiens (human)	Up-frameshift suppressor 2 homolog,hUpf2
3	D, B, F	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
4	F	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	19	Chemie (HOH)

Sequence modifications

C, A, E, G: 58 - 206 (UniProt: Q9H1J1)

PDB	External Database	Details
Met 31	-	initiating methionine
Ser 32	-	expression tag
Tyr 33	-	expression tag
Tyr 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
Asp 41	-	expression tag
Tyr 42	-	expression tag
Asp 43	-	expression tag
Ile 44	-	expression tag
Pro 45	-	expression tag
Thr 46	-	expression tag
Thr 47	-	expression tag
Glu 48	-	expression tag
Asn 49	-	expression tag
Leu 50	-	expression tag
Tyr 51	-	expression tag
Phe 52	-	expression tag
Gln 53	-	expression tag
Gly 54	-	expression tag
Ala 55	-	expression tag
Met 56	-	expression tag
Asp 57	-	expression tag

D, B, F, H: 761 - 1054 (UniProt: Q9HAU5)

PDB	External Database	Details
Met 734	-	initiating methionine
Ser 735	-	expression tag
Tyr 736	-	expression tag
Tyr 737	-	expression tag
His 738	-	expression tag
His 739	-	expression tag
His 740	-	expression tag
His 741	-	expression tag
His 742	-	expression tag
His 743	-	expression tag
Asp 744	-	expression tag
Tyr 745	-	expression tag
Asp 746	-	expression tag
Ile 747	-	expression tag
Pro 748	-	expression tag
Thr 749	-	expression tag
Thr 750	-	expression tag
Glu 751	-	expression tag
Asn 752	-	expression tag
Leu 753	-	expression tag
Tyr 754	-	expression tag
Phe 755	-	expression tag
Gln 756	-	expression tag
Gly 757	-	expression tag
Ala 758	-	expression tag
Met 759	-	expression tag
Asp 760	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	234485.5
Non-Polymers*	Number of molecules	4
	Total formula weight	486.0
All*	Total formula weight	234971.5

*Water molecules are not included.