7Q3N
Cryo-EM of the complex between human uromodulin (UMOD)/Tamm-Horsfall protein (THP) and the FimH lectin domain from uropathogenic E. coli
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | U | Uromodulin | polymer | 563 | 61498.8 | 1 | UniProt (P07911) Pfam (PF12947) Pfam (PF07645) Pfam (PF00100) In PDB | Homo sapiens (Human) | Tamm-Horsfall urinary glycoprotein,THP |
| 2 | F | Type 1 fimbiral adhesin FimH | polymer | 172 | 18669.7 | 1 | UniProt (Q1R2J4) Pfam (PF09160) In PDB | Escherichia coli (strain UTI89 / UPEC) | |
| 3 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 1 | In PDB GlyTouCan (G21290RB) | |||
| 4 | B | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 5 | C | beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1479.3 | 1 | In PDB GlyTouCan (G66937TJ) | |||
| 6 | D | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G55220VL) |
Sequence modifications
F: 1 - 159 (UniProt: Q1R2J4)
| PDB | External Database | Details |
|---|---|---|
| Val 27 | Ala 48 | engineered mutation |
| Ser 160 | - | expression tag |
| His 161 | - | expression tag |
| His 162 | - | expression tag |
| Trp 163 | - | expression tag |
| Gly 164 | - | expression tag |
| His 165 | - | expression tag |
| His 166 | - | expression tag |
| His 167 | - | expression tag |
| His 168 | - | expression tag |
| His 169 | - | expression tag |
| His 170 | - | expression tag |
| His 171 | - | expression tag |
| His 172 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 80168.5 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 3709.4 | |
| All* | Total formula weight | 83877.9 |
