7Q35

Crystal structure of the mutant bacteriorhodopsin pressurized with krypton

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Bacteriorhodopsin	polymer	269	29045.8	1	UniProt (P02945) Pfam (PF01036) In PDB	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)	BR,Bacterioopsin,BO
2	A	RETINAL	non-polymer		284.4	1	Chemie (RET)
3	A	EICOSANE	non-polymer		282.5	11	Chemie (LFA)
4	A	OLEIC ACID	non-polymer		282.5	3	Chemie (OLA)
5	A	HEXANE	non-polymer		86.2	3	Chemie (HEX)
6	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	A	KRYPTON	non-polymer		83.8	35	Chemie (KR)
8		water	water		18.0	106	Chemie (HOH)

Sequence modifications

A: 1 - 249 (UniProt: P02945)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 17	Thr 30	engineered mutation
Ala 24	Thr 37	engineered mutation
Ala 47	Thr 60	engineered mutation
Gly 250	-	expression tag
Ser 251	-	expression tag
Gly 252	-	expression tag
Ile 253	-	expression tag
Glu 254	-	expression tag
Gly 255	-	expression tag
Arg 256	-	expression tag
Ser 257	-	expression tag
Gly 258	-	expression tag
Ala 259	-	expression tag
Pro 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29045.8
Non-Polymers*	Number of molecules	54
	Total formula weight	7527.4
All*	Total formula weight	36573.2

*Water molecules are not included.