7Q35
Crystal structure of the mutant bacteriorhodopsin pressurized with krypton
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8634 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 115.330, 119.986, 36.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.610 - 2.000 |
| R-factor | 0.17238 |
| Rwork | 0.169 |
| R-free | 0.23199 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xxj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.489 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.142 | |
| Rmeas | 0.149 | 1.860 |
| Number of reflections | 33739 | 4700 |
| <I/σ(I)> | 9.09 | 1.49 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.998 | 0.517 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 3.0 M Ammonium Sulfate,0.1 M Sodium Acetate, pH 4.6 |
