7PWO

Cryo-EM structure of Giardia lamblia ribosome at 2.75 A resolution

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	T1	Ribosomal protein S19e	polymer	139	15463.9	1	UniProt (A0A644F964) Pfam (PF01090) In PDB	Giardia lamblia ATCC 50803
2	N1	Ribosomal protein S13	polymer	154	17627.9	1	UniProt (A8BE02) Pfam (PF08069) Pfam (PF00312) In PDB	Giardia lamblia ATCC 50803
3	J1	Ribosomal protein S9	polymer	189	21699.3	1	UniProt (A8BKS8) Pfam (PF00163) Pfam (PF01479) In PDB	Giardia lamblia ATCC 50803
4	D1	Ribosomal protein S3	polymer	217	24779.1	1	UniProt (A8B8Z2) Pfam (PF00189) In PDB	Giardia lamblia ATCC 50803
5	X1	Ribosomal protein S23	polymer	143	15897.8	1	UniProt (A8BS96) Pfam (PF00164) In PDB	Giardia lamblia ATCC 50803
6	S1	Ribosomal protein S18	polymer	154	17510.3	1	UniProt (A8BZ58) Pfam (PF00416) In PDB	Giardia lamblia ATCC 50803
7	Q1	Ribosomal protein S16	polymer	158	17634.6	1	UniProt (A8BKC6) Pfam (PF00380) In PDB	Giardia lamblia ATCC 50803
8	C1	Ribosomal protein S2	polymer	242	26765.7	1	UniProt (A8BMZ1) Pfam (PF00333) Pfam (PF03719) In PDB	Giardia lamblia ATCC 50803
9	B1	40S ribosomal protein S3a	polymer	248	28413.1	1	UniProt (A8BS79) Pfam (PF01015) In PDB	Giardia lamblia ATCC 50803
10	b1	Ribosomal protein S27	polymer	124	14117.3	1	UniProt (A8BJL1) Pfam (PF01667) In PDB	Giardia lamblia ATCC 50803
11	a1	40S ribosomal protein S26	polymer	109	12791.0	1	UniProt (A8BQZ7) Pfam (PF01283) In PDB	Giardia lamblia ATCC 50803
12	V1	40S ribosomal protein S21	polymer	89	9686.1	1	UniProt (A8B484) Pfam (PF01249) In PDB	Giardia lamblia ATCC 50803
13	R1	Ribosomal protein S17	polymer	137	15757.4	1	UniProt (A8BRG5) Pfam (PF00833) In PDB	Giardia lamblia ATCC 50803
14	K1	Ribosomal protein S10B	polymer	134	15406.6	1	UniProt (A8BRX3) Pfam (PF03501) In PDB	Giardia lamblia ATCC 50803
15	I1	40S ribosomal protein S8	polymer	174	19451.6	1	UniProt (A8B252) Pfam (PF01201) In PDB	Giardia lamblia ATCC 50803
16	e1	40S ribosomal protein S30	polymer	69	7932.4	1	UniProt (A0A644F843) Pfam (PF04758) In PDB	Giardia lamblia ATCC 50803
17	H1	40S ribosomal protein S7	polymer	190	21786.5	1	UniProt (A8BT38) Pfam (PF01251) In PDB	Giardia lamblia ATCC 50803
18	n1	Ribosomal protein eL41	polymer	41	5194.3	1	UniProt (V6TX21) Pfam (PF05162) In PDB	Giardia lamblia ATCC 50803
19	c1	Ribosomal protein S28	polymer	64	7358.6	1	UniProt (A8B4F3) Pfam (PF01200) In PDB	Giardia lamblia ATCC 50803
20	O1	Ribosomal protein S14	polymer	145	15756.0	1	UniProt (E2RU83) Pfam (PF00411) In PDB	Giardia lamblia ATCC 50803
21	W1	Ribosomal protein S15A	polymer	130	14770.3	1	UniProt (E2RU77) Pfam (PF00410) In PDB	Giardia lamblia ATCC 50803
22	E1	40S ribosomal protein S4	polymer	268	30464.8	1	UniProt (A8BFE3) Pfam (PF08071) Pfam (PF00900) Pfam (PF16121) In PDB	Giardia lamblia ATCC 50803
23	L1	SSU ribosomal protein S17P	polymer	199	23245.0	1	UniProt (V6TVJ7) Pfam (PF16205) Pfam (PF00366) In PDB	Giardia lamblia ATCC 50803
24	U1	Ribosomal protein S20	polymer	126	14073.2	1	UniProt (A8B4G0) Pfam (PF00338) In PDB	Giardia lamblia ATCC 50803
25	1	rRNA 28S	polymer	2707	878238.5	1		Giardia lamblia ATCC 50803
26	3	rRNA 5S	polymer	120	38671.0	1		Giardia lamblia ATCC 50803
27	42	rRNA 5.8S	polymer	139	44972.0	1	GenBank (9351)	Giardia lamblia ATCC 50803
28	A2	Ribosomal protein L2	polymer	251	27090.5	1	UniProt (E2RTN4) Pfam (PF00181) Pfam (PF03947) In PDB	Giardia lamblia ATCC 50803	Ribosomal protein L8
29	B2	Ribosomal protein L3	polymer	379	42715.5	1	UniProt (A8BRZ3) Pfam (PF00297) In PDB	Giardia lamblia ATCC 50803
30	C2	Ribosomal protein L4	polymer	316	35048.7	1	UniProt (A8B7H8) Pfam (PF00573) Pfam (PF14374) In PDB	Giardia lamblia ATCC 50803
31	D2	Ribosomal protein L5	polymer	297	33961.4	1	UniProt (E2RU47) Pfam (PF17144) Pfam (PF14204) In PDB	Giardia lamblia ATCC 50803
32	F2	Ribosomal protein L7	polymer	235	27103.4	1	UniProt (A8BRY6) Pfam (PF00327) In PDB	Giardia lamblia ATCC 50803
33	G2	60S ribosomal protein L7a	polymer	225	25427.7	1	UniProt (A8BLL7) Pfam (PF01248) In PDB	Giardia lamblia ATCC 50803
34	H2	Ribosomal protein L6	polymer	185	20889.4	1	UniProt (A8BNT5) Pfam (PF00347) In PDB	Giardia lamblia ATCC 50803	Ribosomal protein L9
35	I2	Ribosomal protein L10	polymer	210	24283.2	1	UniProt (A8BM86) Pfam (PF00252) In PDB	Giardia lamblia ATCC 50803
36	J2	Ribosomal protein L11	polymer	173	19767.7	1	UniProt (A8BI95) Pfam (PF00281) Pfam (PF00673) In PDB	Giardia lamblia ATCC 50803
37	L2	60S ribosomal protein L13	polymer	234	26640.3	1	UniProt (A8B6M8) Pfam (PF01294) In PDB	Giardia lamblia ATCC 50803
38	M2	Ribosomal protein L14	polymer	131	14785.5	1	UniProt (A8B4F8) Pfam (PF01929) In PDB	Giardia lamblia ATCC 50803
39	N2	Ribosomal protein L15	polymer	204	24353.7	1	UniProt (A8B8Z6) Pfam (PF00827) In PDB	Giardia lamblia ATCC 50803
40	O2	Ribosomal protein L13a	polymer	197	22921.9	1	UniProt (A8BU75) Pfam (PF00572) In PDB	Giardia lamblia ATCC 50803
41	P2	Ribosomal protein L17	polymer	164	18722.8	1	UniProt (A8BE76) Pfam (PF00237) In PDB	Giardia lamblia ATCC 50803
42	Q2	Ribosomal protein L18	polymer	179	20207.8	1	UniProt (A8B4T7) Pfam (PF17135) In PDB	Giardia lamblia ATCC 50803
43	R2	Ribosomal protein L19	polymer	196	23199.2	1	UniProt (A8BH61) Pfam (PF01280) In PDB	Giardia lamblia ATCC 50803
44	S2	60S ribosomal protein L18a	polymer	173	20624.9	1	UniProt (A8BT04) Pfam (PF01775) In PDB	Giardia lamblia ATCC 50803
45	T2	Ribosomal protein L21	polymer	159	18296.4	1	UniProt (A8B2Q4) Pfam (PF01157) In PDB	Giardia lamblia ATCC 50803
46	U2	Ribosomal protein eL22	polymer	171	19942.4	1	UniProt (A8BW84) Pfam (PF01776) In PDB	Giardia lamblia ATCC 50803
47	V2	Ribosomal protein L23	polymer	142	15450.0	1	UniProt (A8BS37) Pfam (PF00238) In PDB	Giardia lamblia ATCC 50803
48	X2	Ribosomal protein L23A	polymer	141	16053.9	1	UniProt (A8BMP6) Pfam (PF03939) Pfam (PF00276) In PDB	Giardia lamblia ATCC 50803
49	Y2	Ribosomal protein L26	polymer	135	15621.4	1	UniProt (A8BFT3) Pfam (PF16906) In PDB	Giardia lamblia ATCC 50803
50	Z2	60S ribosomal protein L27	polymer	135	15374.9	1	UniProt (A8B1T3) Pfam (PF00467) Pfam (PF01777) In PDB	Giardia lamblia ATCC 50803
51	a2	Ribosomal protein L27a	polymer	149	17167.0	1	UniProt (A8BZ78) Pfam (PF00828) In PDB	Giardia lamblia ATCC 50803
52	b2	60S ribosomal protein L29	polymer	62	7363.6	1	UniProt (A8BYY2) Pfam (PF01779) In PDB	Giardia lamblia ATCC 50803
53	c2	Ribosomal protein L30	polymer	109	11698.6	1	UniProt (A8B5N7) Pfam (PF01248) In PDB	Giardia lamblia ATCC 50803
54	d2	Ribosomal protein L31B	polymer	106	12184.1	1	UniProt (A8BMP3) Pfam (PF01198) In PDB	Giardia lamblia ATCC 50803	Ribosomal protein L31e
55	e2	Ribosomal protein L32	polymer	136	15986.0	1	UniProt (A8BMD9) Pfam (PF01655) In PDB	Giardia lamblia ATCC 50803
56	f2	Ribosomal protein L35a	polymer	123	13913.1	1	UniProt (A8BKK5) Pfam (PF01247) In PDB	Giardia lamblia ATCC 50803
57	g2	Ribosomal protein L34	polymer	120	13528.8	1	UniProt (A8BMF7) Pfam (PF01199) In PDB	Giardia lamblia ATCC 50803
58	h2	Ribosomal protein L35	polymer	124	14292.2	1	UniProt (A8BRG7) Pfam (PF00831) In PDB	Giardia lamblia ATCC 50803
59	i2	Ribosomal protein L36-1	polymer	90	10586.7	1	UniProt (A8BYA6) Pfam (PF01158) In PDB	Giardia lamblia ATCC 50803	Ribosomal protein L36e
60	j2	Ribosomal protein L37	polymer	89	10329.0	1	UniProt (A8BLV7) Pfam (PF01907) In PDB	Giardia lamblia ATCC 50803
61	k2	Ribosomal L38e	polymer	77	8605.1	1	UniProt (A8B481) Pfam (PF01781) In PDB	Giardia lamblia ATCC 50803
62	l2	Ribosomal protein L39	polymer	51	6353.7	1	UniProt (A0A644EZB9) Pfam (PF00832) In PDB	Giardia lamblia ATCC 50803
63	m2	Ubiquitin/Ribosomal protein L40e	polymer	127	14343.7	1	UniProt (A8BXE1) Pfam (PF00240) Pfam (PF01020) In PDB	Giardia lamblia ATCC 50803	Ubiquitin/ribosomal protein CEP52
64	o2	Ribosomal protein L44	polymer	106	12311.7	1	UniProt (A8BQU8) Pfam (PF00935) In PDB	Giardia lamblia ATCC 50803
65	p2	Ribosomal protein L37a	polymer	94	10508.4	1	UniProt (A8BN26) Pfam (PF01780) In PDB	Giardia lamblia ATCC 50803
66	w2	E-site tRNA	polymer	14	4459.8	1		Giardia lamblia ATCC 50803
67	W2	Ribosomal protein L24A	polymer	102	12005.2	1	UniProt (A8BRH7) Pfam (PF01246) In PDB	Giardia lamblia ATCC 50803
68	E	P-site tRNA	polymer	3	894.6	1		Giardia lamblia ATCC 50803
69	d1	Ribosomal protein S29A	polymer	137	15499.8	1	UniProt (A8B7H6) Pfam (PF00253) In PDB	Giardia lamblia ATCC 50803
70	F1	Ribosomal protein S5	polymer	190	21058.3	1	UniProt (A8BZD3) Pfam (PF00177) In PDB	Giardia lamblia ATCC 50803
71	Y1	Ribosomal protein S24	polymer	132	14872.6	1	UniProt (A8B593) Pfam (PF01282) In PDB	Giardia lamblia ATCC 50803
72	G1	40S ribosomal protein S6	polymer	248	28084.1	1	UniProt (A8B6L8) Pfam (PF01092) In PDB	Giardia lamblia ATCC 50803
73	2	rRNA 18S	polymer	1452	471124.5	1		Giardia lamblia ATCC 50803
74	A1	40S ribosomal protein SA	polymer	245	28101.8	1	UniProt (A8BE86) Pfam (PF00318) In PDB	Giardia lamblia ATCC 50803
75	X1, 1, B2, 2, S1...	POTASSIUM ION	non-polymer		39.1	455	Chemie (K)
76	1, 2, C2, F2, P2...	MAGNESIUM ION	non-polymer		24.3	101	Chemie (MG)

Sequence modifications

L1: 2 - 200 (UniProt: V6TVJ7)

PDB	External Database	Details
Ser 12	Gly 35	conflict

Contents of the asymmetric unit

Polymers	Number of chains	74
	Total formula weight	2679219.4
Non-Polymers*	Number of molecules	556
	Total formula weight	20244.4
All*	Total formula weight	2699463.8

*Water molecules are not included.