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- EMDB-13683: Cryo-EM structure of Giardia lamblia ribosome at 2.75 A resolution -

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Basic information

Entry
Database: EMDB / ID: EMD-13683
TitleCryo-EM structure of Giardia lamblia ribosome at 2.75 A resolution
Map data
Sample
  • Complex: Giardia lamblia ribosome
    • Protein or peptide: x 68 types
    • RNA: x 6 types
  • Ligand: x 2 types
Keywordsribosome / giardia / parasite
Function / homology
Function and homology information


endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maturation of SSU-rRNA / positive regulation of apoptotic signaling pathway / small-subunit processome ...endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maturation of SSU-rRNA / positive regulation of apoptotic signaling pathway / small-subunit processome / modification-dependent protein catabolic process / rRNA processing / protein tag activity / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol
Similarity search - Function
Ribosomal protein L40e, archaeal / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily ...Ribosomal protein L40e, archaeal / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L29e / Ribosomal L29e protein family / 40S Ribosomal protein S10 / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S6, eukaryotic / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein L34Ae / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L34e / Ribosomal protein L18e signature. / Ribosomal protein S24e / Ribosomal protein S23, eukaryotic/archaeal / 60S ribosomal protein L19 / Ribosomal protein S24e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein S27 / Ribosomal protein L30/YlxQ / 60S ribosomal protein L35 / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L7A/L8 / Ribosomal protein S3Ae / Ribosomal protein S28e conserved site / Ribosomal S3Ae family
Similarity search - Domain/homology
Ribosomal protein L39 / 40S ribosomal protein S30 / Ribosomal protein S19e / Ribosomal protein L27 / 40S ribosomal protein S8 / Ribosomal protein L21 / Ribosomal L38e / 40S ribosomal protein S21 / Ribosomal protein S28 / Ribosomal protein L14 ...Ribosomal protein L39 / 40S ribosomal protein S30 / Ribosomal protein S19e / Ribosomal protein L27 / 40S ribosomal protein S8 / Ribosomal protein L21 / Ribosomal L38e / 40S ribosomal protein S21 / Ribosomal protein S28 / Ribosomal protein L14 / Ribosomal protein S20 / Ribosomal protein L18 / Ribosomal protein S24 / Ribosomal protein L30 / 40S ribosomal protein S6 / 60S ribosomal protein L13 / Ribosomal protein S29A / Ribosomal protein L4 / 40S ribosomal protein S3 / Ribosomal protein L15 / Ribosomal protein S13 / Ribosomal protein L17 / Small ribosomal subunit protein uS2 / 40S ribosomal protein S4 / Ribosomal protein L26 / Ribosomal protein L19 / Ribosomal protein L11 / Ribosomal protein S27 / Ribosomal protein S16 / Ribosomal protein L35a / Ribosomal protein S9 / 60S ribosomal protein L7a / Ribosomal protein L37 / Ribosomal protein L10 / Ribosomal protein L32 / Large ribosomal subunit protein eL34 / Ribosomal protein L31B / Ribosomal protein L23A / Ribosomal protein S2 / Ribosomal protein L37a / Ribosomal protein L6 / Ribosomal protein L44 / 40S ribosomal protein S26 / Ribosomal protein S17 / Ribosomal protein L35 / Ribosomal protein L24A / Ribosomal protein S10B / Ribosomal protein L7 / Ribosomal protein L3 / Ribosomal protein L23 / Small ribosomal subunit protein eS1 / Ribosomal protein S23 / 60S ribosomal protein L18a / 40S ribosomal protein S7 / Ribosomal protein L13a / Large ribosomal subunit protein eL22 / 50S ribosomal protein L40e / Ribosomal protein L36-1 / 60S ribosomal protein L29 / Ribosomal protein S18 / Ribosomal protein L27a / Ribosomal protein S5 / Ribosomal protein L2 / Ribosomal protein L5 / Ribosomal protein S15A / Ribosomal protein S14 / SSU ribosomal protein S17P / 60S ribosomal protein L41
Similarity search - Component
Biological speciesGiardia lamblia ATCC 50803 (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.75 Å
AuthorsHiregange DG / Rivalta A / Bose T / Breiner-Goldstein E / Samiya S / Cimicata G / Kulakova L / Zimmerman E / Bashan A / Herzberg O / Yonath A
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI119788 United States
CitationJournal: Nucleic Acids Res / Year: 2022
Title: Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
Authors: Disha-Gajanan Hiregange / Andre Rivalta / Tanaya Bose / Elinor Breiner-Goldstein / Sarit Samiya / Giuseppe Cimicata / Liudmila Kulakova / Ella Zimmerman / Anat Bashan / Osnat Herzberg / Ada Yonath /
Abstract: Giardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs is spreading, new strategies for combating ...Giardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs is spreading, new strategies for combating giardiasis need to be developed. The G. lamblia ribosome may provide a promising therapeutic target due to its distinct sequence differences from ribosomes of most eukaryotes and prokaryotes. Here, we report the cryo-electron microscopy structure of the G. lamblia (WB strain) ribosome determined at 2.75 Å resolution. The ribosomal RNA is the shortest known among eukaryotes, and lacks nearly all the eukaryote-specific ribosomal RNA expansion segments. In contrast, the ribosomal proteins are typically eukaryotic with some species-specific insertions/extensions. Most typical inter-subunit bridges are maintained except for one missing contact site. Unique structural features are located mainly at the ribosome's periphery. These may be exploited as target sites for the design of new compounds that inhibit selectively the parasite's ribosomal activity.
History
DepositionOct 7, 2021-
Header (metadata) releaseApr 20, 2022-
Map releaseApr 20, 2022-
UpdateApr 24, 2024-
Current statusApr 24, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13683.png

Downloads & links

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Map

FileDownload / File: emd_13683.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
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AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 440 pix.
= 374. Å		0.85 Å/pix.
x 440 pix.
= 374. Å		0.85 Å/pix.
x 440 pix.
= 374. Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.85 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0055
Minimum - Maximum-0.030112306 - 0.08726277
Average (Standard dev.)0.0002469946 (±0.0034110902)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 374.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_13683_additional_1.map
Projections & Slices
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Sample components

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Entire : Giardia lamblia ribosome

EntireName: Giardia lamblia ribosome
Components
  • Complex: Giardia lamblia ribosome
    • Protein or peptide: Ribosomal protein S19e
    • Protein or peptide: Ribosomal protein S13
    • Protein or peptide: Ribosomal protein S9
    • Protein or peptide: Ribosomal protein S3
    • Protein or peptide: Ribosomal protein S23
    • Protein or peptide: Ribosomal protein S18
    • Protein or peptide: Ribosomal protein S16
    • Protein or peptide: Ribosomal protein S2
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: Ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: Ribosomal protein S17
    • Protein or peptide: Ribosomal protein S10B
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: Ribosomal protein eL41
    • Protein or peptide: Ribosomal protein S28
    • Protein or peptide: Ribosomal protein S14
    • Protein or peptide: Ribosomal protein S15A
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: SSU ribosomal protein S17P
    • Protein or peptide: Ribosomal protein S20
    • RNA: rRNA 28S
    • RNA: rRNA 5S
    • RNA: rRNA 5.8S
    • Protein or peptide: Ribosomal protein L2
    • Protein or peptide: Ribosomal protein L3
    • Protein or peptide: Ribosomal protein L4
    • Protein or peptide: Ribosomal protein L5
    • Protein or peptide: Ribosomal protein L7
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: Ribosomal protein L6
    • Protein or peptide: Ribosomal protein L10
    • Protein or peptide: Ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: Ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: Ribosomal protein L13a
    • Protein or peptide: Ribosomal protein L17
    • Protein or peptide: Ribosomal protein L18
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: Ribosomal protein L21
    • Protein or peptide: Ribosomal protein eL22
    • Protein or peptide: Ribosomal protein L23
    • Protein or peptide: Ribosomal protein L23A
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: Ribosomal protein L27a
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: Ribosomal protein L30
    • Protein or peptide: Ribosomal protein L31B
    • Protein or peptide: Ribosomal protein L32
    • Protein or peptide: Ribosomal protein L35a
    • Protein or peptide: Ribosomal protein L34
    • Protein or peptide: Ribosomal protein L35
    • Protein or peptide: Ribosomal protein L36-1
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: Ribosomal L38e
    • Protein or peptide: Ribosomal protein L39
    • Protein or peptide: Ubiquitin/Ribosomal protein L40e
    • Protein or peptide: Ribosomal protein L44
    • Protein or peptide: Ribosomal protein L37a
    • RNA: E-site tRNA
    • Protein or peptide: Ribosomal protein L24A
    • RNA: P-site tRNA
    • Protein or peptide: Ribosomal protein S29A
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: Ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S6
    • RNA: rRNA 18S
    • Protein or peptide: 40S ribosomal protein SA
  • Ligand: POTASSIUM ION
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Giardia lamblia ribosome

SupramoleculeName: Giardia lamblia ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#74
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)

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Macromolecule #1: Ribosomal protein S19e

MacromoleculeName: Ribosomal protein S19e / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.46391 KDa
SequenceString:
MSSVTRVPAD VFINSFAAHL KNRGIIKCPA FTDYVKTGVS RQYAPRDADW FYIKAASVIR HFYISGSHSI GVAGLARKYS SLQKGKTTP HHTKRASCKV IRSIVSQFLG QKLLIAGERG RHISPNGRKM VEDFAEGLQK

UniProtKB: Ribosomal protein S19e

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Macromolecule #2: Ribosomal protein S13

MacromoleculeName: Ribosomal protein S13 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 17.627873 KDa
SequenceString:
MGRMHSKGKG ISRSAIPYTR APAPWNKYTP EDVCEQICKL AKKGTPPSRI GLILRDQFAI GQVKNVTGSK IVRILKAKGL APEIPEDLY CMIKKAVAIR KHLERSRRDK DSKFRLILVE SRIHRLARYY RQARRLPPTF RYKSDQAAAL LATYA

UniProtKB: Ribosomal protein S13

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Macromolecule #3: Ribosomal protein S9

MacromoleculeName: Ribosomal protein S9 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 21.699322 KDa
SequenceString:
MPRISRHRNS SKTYSTPKKP FTKPRLDAEL KLCGEYGLKC KREIYRARYM LAKIRRTARN LLTLDENDIK RRFEGAALLR RLHMLGILD EEKDQLDYVL GLRIEDIFKR RLQSVIVESK LAKSVHHARN MIKARHISVG GQIVNIPSYM VRISNTSRVA I ADTSSLAP NGKPGRTKRA AARRQSSGDN E

UniProtKB: Ribosomal protein S9

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Macromolecule #4: Ribosomal protein S3

MacromoleculeName: Ribosomal protein S3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 24.779148 KDa
SequenceString: MPRIVRRDEE NVYYSKYLND CIMEAELHEF FSRELVDAGY ASCSLCRTID SICVIIQCAE PEVVMGDNHY RLHKIEYLLA GRFMCDPKN FNIWVDKIIK RGLSAEIMVD NLRAKLEEAM PIRRAAYSII RTAMRAGAAG CEVIVAGKLR AQRARANKFK E GCLISTGH ...String:
MPRIVRRDEE NVYYSKYLND CIMEAELHEF FSRELVDAGY ASCSLCRTID SICVIIQCAE PEVVMGDNHY RLHKIEYLLA GRFMCDPKN FNIWVDKIIK RGLSAEIMVD NLRAKLEEAM PIRRAAYSII RTAMRAGAAG CEVIVAGKLR AQRARANKFK E GCLISTGH PKRIFLAEAT RHIKMRQGVI GVKVRIYNPN IRGAVMPDKI EIGAFSRQK

UniProtKB: 40S ribosomal protein S3

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Macromolecule #5: Ribosomal protein S23

MacromoleculeName: Ribosomal protein S23 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.89784 KDa
SequenceString:
MSKTSGLYTA RKLRTVRRVN RWADKKYRKA HLGTWLKANP LEGACMARGI VTQKIGISAK QPNSAVRKAV RVQLTKNSKK ITAFVPRDG SITYIDNNDE VLVAGFGRAG RAVGDIPGCR FKVIKVAGIG LLALFLRKRE KRAR

UniProtKB: Ribosomal protein S23

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Macromolecule #6: Ribosomal protein S18

MacromoleculeName: Ribosomal protein S18 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 17.510338 KDa
SequenceString:
MTTIVQPNDY RDLLRMFNTN IQGKVVLECA LVGIKGIGRR FANVALKKAN IDVHKRAGEL TAAEEDALMK VLEDPLSHGI PAWMLNRNN DFESGKDMHI LVNTIASTHR ADLERLRKTR CERGVRHHHN LKVRGQHTKS TGRRGHVVGV QRKKK

UniProtKB: Ribosomal protein S18

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Macromolecule #7: Ribosomal protein S16

MacromoleculeName: Ribosomal protein S16 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 17.634584 KDa
SequenceString:
MAKGNRRDLL ERNDIEVRER DKYTVHTLGK KKNSVAVAFV KAGKGDILVN NVPLALVRPE ILRTKVSEPI MILGESLFKG LDMNIKVAG GGHVSQIYAV RQAISRGVLA SVKDEAARSE LRAKLVSYDR QLVVSDPRRC EAKKFGGRGA RARRQKSYR

UniProtKB: Ribosomal protein S16

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Macromolecule #8: Ribosomal protein S2

MacromoleculeName: Ribosomal protein S2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 26.765695 KDa
SequenceString: MQAESAPRPE RPERGERGPR QMEEEEWHPS TKLGRLVKAG KIESLYDIFK YSLPIKETEI IDYFFPKSEL AEEVCNIMSV QKQTTAGQR TRFRAHVIVG NRDGFIGYGA GVSKEVANAI KKAAKNARLN IVPVRRGFWG GKLGDPHTVS SKLSGKCGSV R VRLIPAPR ...String:
MQAESAPRPE RPERGERGPR QMEEEEWHPS TKLGRLVKAG KIESLYDIFK YSLPIKETEI IDYFFPKSEL AEEVCNIMSV QKQTTAGQR TRFRAHVIVG NRDGFIGYGA GVSKEVANAI KKAAKNARLN IVPVRRGFWG GKLGDPHTVS SKLSGKCGSV R VRLIPAPR GAGIVASPTV AKVLEFAGVS DVFTRQSGHS RTLMNSVGAV YNALKSSYSI LTPDLWKKEQ LDHVQDIRTM SL AD

UniProtKB: Ribosomal protein S2

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Macromolecule #9: 40S ribosomal protein S3a

MacromoleculeName: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 28.413148 KDa
SequenceString: MAIGGNKTGK KKITQRKNPD PYLKKEWYPV IAPAHFKSRH IGQTPVTKTA GLRVATDVIN HRVYEVNLGD LNPGAEDLNG NTKFYLQTQ LVSDGKCLTN FHGMDITRHK YGSLFRKGCD CIDVFMDIPT TDGYLLRVFV IAFTDRFRFQ RRKNCHVKGR V IRMMRAGI ...String:
MAIGGNKTGK KKITQRKNPD PYLKKEWYPV IAPAHFKSRH IGQTPVTKTA GLRVATDVIN HRVYEVNLGD LNPGAEDLNG NTKFYLQTQ LVSDGKCLTN FHGMDITRHK YGSLFRKGCD CIDVFMDIPT TDGYLLRVFV IAFTDRFRFQ RRKNCHVKGR V IRMMRAGI YETLHNELGK VSIPVLVTKL SNFEVNAKLT EALQKITMCR DVMIRRVKVV KRPRNTMELL STMHDSNQVR NL AEGQSIQ Q

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #10: Ribosomal protein S27

MacromoleculeName: Ribosomal protein S27 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.117312 KDa
SequenceString:
MTPFSRFGEH CQLNINHAVY TLKLQTNPNK FKFKSELRGA KVSMNVDLLY PDPVEEQRKH KLKRLVQGPN SYFLEIKCAG CQDIVVVYS HANTPVFCPG CTSQISIPTG GKCAFTSQVE RYRQK

UniProtKB: Ribosomal protein S27

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Macromolecule #11: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 12.791023 KDa
SequenceString:
MPVKRRNNGR SKYNCGHTNI VRCQNCHRCV PKDKVIKRFT IRNIVDNTIA DDVLNACVIQ GFAIPKLYNK VQYCVSCSIH NHIVRVRSR EDRKIRTPPK RFPRNSSQKK

UniProtKB: 40S ribosomal protein S26

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Macromolecule #12: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 9.686149 KDa
SequenceString:
MQSDDIGMKN SSGVVVDAYI PRKCSATNRL IGPKDHAAVQ LSVARVNDKG KMIENNYVTF AFCGAIRSRG GVDSAFMLLA QEHGLMERL

UniProtKB: 40S ribosomal protein S21

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Macromolecule #13: Ribosomal protein S17

MacromoleculeName: Ribosomal protein S17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.757351 KDa
SequenceString:
MGKVRTKTIK RAARKIVENN YESLKPKDFQ NNKAICSKRN VAIIPSKRLR NKVAGYVTTL MNRVVKGPVH GISLRLQEEE RERRDNYAP KKSEFDVENI TVSGATQQMI DSIGFKCMST SLKVIDPTMR KRARDTRE

UniProtKB: Ribosomal protein S17

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Macromolecule #14: Ribosomal protein S10B

MacromoleculeName: Ribosomal protein S10B / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.406633 KDa
SequenceString:
MVHIPKAVYK AVYAHLYENA SLALKEYWRV GNVPGITYVD ENGKLCNPTY LHVNCIMQSL ESRGVVKDTF AWRHHYYLLT EKGEQFIRK ELDLTDDVLP TPYSKPSIKP PQYAQCFSSK GKPDRAPQPK PDEAK

UniProtKB: Ribosomal protein S10B

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Macromolecule #15: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 19.451555 KDa
SequenceString:
MGITHDKMYK RSKTGAVQGK WHKKRKYNLG RQPANTKLIV KVTSKKNEGQ KISTIRCRGG NIKRRALRLS HGSFAWGSAD ISHKARILQ VVYNSTSNEL VRTNTLVKGC VVQIDSAPFK SALSNDTLDK EILDQIRGDH ILARISSRPG QVGTADGYIL E GPELLFYQ KKLAPK

UniProtKB: 40S ribosomal protein S8

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Macromolecule #16: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 7.932407 KDa
SequenceString:
MARAHGGLTS AGKVRKCTPK KEKKEKPRPP RGRAYKRLLY NKNFVDDTLI HNGRRLGPNN LLIRQKLGF

UniProtKB: 40S ribosomal protein S30

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Macromolecule #17: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 21.786453 KDa
SequenceString:
MTFPAQRKIR KPFEKLSELE KQVAGTLLKL EDDPDLREMG LGTLYISSVK EIAVDGDKKA AVVYVPYPML QSFQKNSEAL ILKLEKNHS GHRFIIVANR TIMKNAYVFT GKKSIRRPRS RTMTSVYNCI LDDICYPIHV IGKRTLYPAG SNPRLQVVLP K AVYNEVSE RTKTYSAVYK ALTERDTEFI FM

UniProtKB: 40S ribosomal protein S7

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Macromolecule #18: Ribosomal protein eL41

MacromoleculeName: Ribosomal protein eL41 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 5.194272 KDa
SequenceString:
MNTEKPCTEE NFENEIMRAK WAKKRMRKLK RKRRHMKARS K

UniProtKB: 60S ribosomal protein L41

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Macromolecule #19: Ribosomal protein S28

MacromoleculeName: Ribosomal protein S28 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 7.358642 KDa
SequenceString:
MPEPINARVI EVLGRTSSRG GITQVKVEFM TGEKRQIIRN VIGPVRKDDI LVLMESEREA RRLR

UniProtKB: Ribosomal protein S28

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Macromolecule #20: Ribosomal protein S14

MacromoleculeName: Ribosomal protein S14 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.755995 KDa
SequenceString:
MSRKVTYAPE NLPAKNNNSE AFAVCHIYAG KNDTFIHVTD LSGRETIGRV SGGMKVKRHC DEPSAYAAMQ ATQDLIEIIT DRGITAVHI KIRADGGTGK RVYGQGGSAA IRALVRGGIK IGRIEDSTPI PTDRTRHKGG RRGRRL

UniProtKB: Ribosomal protein S14

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Macromolecule #21: Ribosomal protein S15A

MacromoleculeName: Ribosomal protein S15A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.770346 KDa
SequenceString:
MVRINVLRDA LKSICNAQRI GKKQVIVRPS SKVIIEFLQL MQKNGYISDF AVVDNHRSNR IVVNLIGRLN KAGVISPRFD IPANDIEKW VVNLLPSRLF GHIILTTSQG IMDHIEAQHR QIGGKVIGYF Y

UniProtKB: Ribosomal protein S15A

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Macromolecule #22: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 30.464781 KDa
SequenceString: MARGVRKHLK RLNAPKHWLL DKMGGIWAPR PTNGPHGLRE CIPLILILRN RLHYANTYAE TSMILQDKNV LIDGKPRTDP TFPVGFMDV FEIPKVHKTF RVLYDVKGRF TLVPIQSNEA GFKLCRVQKI FLGDKGMPYL STHDARTIRF PHPDIKTNDT I KINLKTGK ...String:
MARGVRKHLK RLNAPKHWLL DKMGGIWAPR PTNGPHGLRE CIPLILILRN RLHYANTYAE TSMILQDKNV LIDGKPRTDP TFPVGFMDV FEIPKVHKTF RVLYDVKGRF TLVPIQSNEA GFKLCRVQKI FLGDKGMPYL STHDARTIRF PHPDIKTNDT I KINLKTGK IDEWYKFDIG KIVMVTGGRN CGRIGTIQAI DKHMGSYTMI RMKDTEGTEF LTRLCNVFVI GNDSPAVAIP TT KGIRPDI IKNRELRLRS IAKRGGGASY E

UniProtKB: 40S ribosomal protein S4

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Macromolecule #23: SSU ribosomal protein S17P

MacromoleculeName: SSU ribosomal protein S17P / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 23.244973 KDa
SequenceString: MSATKDKPQD SATAKVNGRH PLRPDPDRER RALERAENLR KAAYEMAENQ HERAYHKQSQ IFQGRFLRKD NARCLRFYKS IGMGYHTPI TAINGTYIDK KCPFTGNVSI RGRILRGVVH SAKMQRSIIV RRDYLHFVQK YKRYERRHKH VAAHLSPCFR V KEGDIVTI ...String:
MSATKDKPQD SATAKVNGRH PLRPDPDRER RALERAENLR KAAYEMAENQ HERAYHKQSQ IFQGRFLRKD NARCLRFYKS IGMGYHTPI TAINGTYIDK KCPFTGNVSI RGRILRGVVH SAKMQRSIIV RRDYLHFVQK YKRYERRHKH VAAHLSPCFR V KEGDIVTI GECRPLSKTI RFNVIKVEPV SEAAKKRFSP F

UniProtKB: SSU ribosomal protein S17P

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Macromolecule #24: Ribosomal protein S20

MacromoleculeName: Ribosomal protein S20 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.073192 KDa
SequenceString:
MAESVSTGGV NLADHKDGKD MHQKYTIIVV GKDLKLVERY VKSIVKEGRA FGISGPCRAP VRRLTITTRK SPCGEGTNTW HRFELRMYK RTFWVTCGGN DISTILSKTV VIPGIQVTVE VEEPDNE

UniProtKB: Ribosomal protein S20

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Macromolecule #28: Ribosomal protein L2

MacromoleculeName: Ribosomal protein L2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 27.090543 KDa
SequenceString: MGRRIRVQRK GAGSVFRAHV ARRLGAAKLR ANDFAERTGS VRGVVKAILH DRGRGAPLAR VQFPNMRGEG RVNELFIAPE GMYTGQEVF SGNKATLHIG NILPVGNIPE GTYVCNVEEK PMDRGCLARA SGTYCLVVAH NMETNKTRIR LPSGQKKLIS S KARAMIGL ...String:
MGRRIRVQRK GAGSVFRAHV ARRLGAAKLR ANDFAERTGS VRGVVKAILH DRGRGAPLAR VQFPNMRGEG RVNELFIAPE GMYTGQEVF SGNKATLHIG NILPVGNIPE GTYVCNVEEK PMDRGCLARA SGTYCLVVAH NMETNKTRIR LPSGQKKLIS S KARAMIGL VAGGGRTDKP LLKAGNAYHK YKAKRNCWPV VRGVAMNPVD HPHGGGNHQH IGVSSCVPRN AVPGQKVGLI AA RRTGCSG GKRR

UniProtKB: Ribosomal protein L2

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Macromolecule #29: Ribosomal protein L3

MacromoleculeName: Ribosomal protein L3 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 42.715543 KDa
SequenceString: MSHRKFSCCR KGNLGYLPRK RCTRGRGRCK TFPPDVQGAP IHLTAFLGYK AGCTHVMRFI DHRGSNLHNR QAIDQATIID APPMICTAI IGYAKTPKGL RAVTTVWAAH IAEPAMRRFY RNYFHAEKTA FSTYMKKAAD GTYIKEQLGR LKQHADVIRI V AHTQPALT ...String:
MSHRKFSCCR KGNLGYLPRK RCTRGRGRCK TFPPDVQGAP IHLTAFLGYK AGCTHVMRFI DHRGSNLHNR QAIDQATIID APPMICTAI IGYAKTPKGL RAVTTVWAAH IAEPAMRRFY RNYFHAEKTA FSTYMKKAAD GTYIKEQLGR LKQHADVIRI V AHTQPALT PLKQKKADIM EIQVNGGANV AEKVDYAYAL MEKPISIKDV FSVGAQIDTI SITRGRGFEG VVTRWGVTRL PR KTRRGNR KVACIGAWHP ANVQYTVARA GQMGYFHRTD TNKQVFMIDT AENPRCCTTE FDLTDKSINP VGGFVNYGRV QGD FIMIKG TCPGPKRRPV VMRNALLPHR NCPPVQLQWI STASKFGHGI FETVEERRQF YAK

UniProtKB: Ribosomal protein L3

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Macromolecule #30: Ribosomal protein L4

MacromoleculeName: Ribosomal protein L4 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 35.048707 KDa
SequenceString: MNPTVKVFGP TGTQVAELPR PKVFNVPLRP DIINFVHTQL RKCLRTPYAV SRYAGVQCTA HSWGPGRAVA RLPRKHGGIG AYANFARGG HMAHPTTVIR RWCRKVNLNQ RRYAVASALA ASANAALVEA RGHRIADVPS IPLVVDTDNV TKTKDALAII K AVGVLRDV ...String:
MNPTVKVFGP TGTQVAELPR PKVFNVPLRP DIINFVHTQL RKCLRTPYAV SRYAGVQCTA HSWGPGRAVA RLPRKHGGIG AYANFARGG HMAHPTTVIR RWCRKVNLNQ RRYAVASALA ASANAALVEA RGHRIADVPS IPLVVDTDNV TKTKDALAII K AVGVLRDV ERCKDSRHIR AGRGKMRNRR YITRKGPLVI YNTEDVARGF RNLPGVDLCH VSSIRLLELA PGSHPGRLIL WT KSAFASL DDVYAAKQNY TLPRSCITQT DIERILQSDA VQKTFVPKRD PLVIERKVDP FSSKEALARL DPSGTVMPK

UniProtKB: Ribosomal protein L4

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Macromolecule #31: Ribosomal protein L5

MacromoleculeName: Ribosomal protein L5 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 33.961375 KDa
SequenceString: MGWVKVQKTR SYFKRFQVQF RRRREGKTDY QARKALIIQD KNKYATPRYR FVVRRTNKDI ICQVTAAELT KDRVVCAAYS HELPRYGIP VGLTNYAAAY ATGLLCGRRL LEKLSVGIKE NALAQLYPGL DKATGEEYHP DENENEEVHA FKAFLDIGLA R ASTGARVF ...String:
MGWVKVQKTR SYFKRFQVQF RRRREGKTDY QARKALIIQD KNKYATPRYR FVVRRTNKDI ICQVTAAELT KDRVVCAAYS HELPRYGIP VGLTNYAAAY ATGLLCGRRL LEKLSVGIKE NALAQLYPGL DKATGEEYHP DENENEEVHA FKAFLDIGLA R ASTGARVF AAMKGAVDAG LNIPHSMKRF PGYSKDGFDS SALRDRIMGK HVGDYMAELK AEDEETYRKR FSKYIAAKVE PL RLEAMYT KAHAAIRANP QAVKAKFEIK KPVRQARLTL KERKERIDAK KLEALKQLTK

UniProtKB: Ribosomal protein L5

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Macromolecule #32: Ribosomal protein L7

MacromoleculeName: Ribosomal protein L7 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 27.103406 KDa
SequenceString: MPIPELVLKH RRKVQRDIRS AAERATEKEK RAALVKDHAV KSAQVYARYY IDLVESEKKA RENALKNNQL YVPDEPKVYL VVRIRGIVG VAPKVRTILS LLRLRQINNA VFMRCNKATL NALRIVDHYV TYGEPTVETI RNLIYKRGFA KVNGQRIPIV D NNLIDEQL ...String:
MPIPELVLKH RRKVQRDIRS AAERATEKEK RAALVKDHAV KSAQVYARYY IDLVESEKKA RENALKNNQL YVPDEPKVYL VVRIRGIVG VAPKVRTILS LLRLRQINNA VFMRCNKATL NALRIVDHYV TYGEPTVETI RNLIYKRGFA KVNGQRIPIV D NNLIDEQL SKHNILCAED LVHEIFTCGS SFKEANNFLW PFQLNSAALK DKRNNFAEGG DFGYRGKYIN EFVARMI

UniProtKB: Ribosomal protein L7

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Macromolecule #33: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 25.427725 KDa
SequenceString: MSKVSGSDIK RALAVPENKS RSKCDFDLTP FVRWPRQVRI QRQKAVLQRR LKVPPTVNQF MNPISRNLTN EIFNLARKYS PESKEEHKA RLLQIADAKA NGKPLPEKSD KLVIASGIRR ITSLVESKRA KLVLIANDVD PLELVLWLPT LCHKMGVPYA I VRTKGDLG ...String:
MSKVSGSDIK RALAVPENKS RSKCDFDLTP FVRWPRQVRI QRQKAVLQRR LKVPPTVNQF MNPISRNLTN EIFNLARKYS PESKEEHKA RLLQIADAKA NGKPLPEKSD KLVIASGIRR ITSLVESKRA KLVLIANDVD PLELVLWLPT LCHKMGVPYA I VRTKGDLG KLVHLKKTTS VCFTDVNPED KPTFDKILAA VAHEVDYAKA MKTYGGGVRR EDEAQQM

UniProtKB: 60S ribosomal protein L7a

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Macromolecule #34: Ribosomal protein L6

MacromoleculeName: Ribosomal protein L6 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 20.889379 KDa
SequenceString:
MPLCSSMTIK VPTDVECTIN KRVVTVKGKK GTLVRDFSHA PLDITHANDT ISVAVWFPRG KRNALPRTIC SHIENMFKGV TYGFEYKMR LAYAHFPIAA TVVDNNKAIE IRNFMHQIKT RRIECLPGVT IAMSTNVKDE LILRGISIED VSHTAARIHE S LKVPNKDL RKFLDGCYVS SRGLQEQ

UniProtKB: Ribosomal protein L6

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Macromolecule #35: Ribosomal protein L10

MacromoleculeName: Ribosomal protein L10 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 24.283172 KDa
SequenceString: MGRRPARCYR YQKNKPYPKS RFCRGVPDPK IRYFDLGNRR AKVNDFPYCF HLLSGEKEQV SSEALEACRI ACNKYIAKKA GKDSFHLRI RVHPFHVIRI NKMLSCAGAD RLQTGMRGAW GKPQGTVARV SIGQPLLSVR CRASAKDYVK DALRRAKFKI P GRQAIVES ...String:
MGRRPARCYR YQKNKPYPKS RFCRGVPDPK IRYFDLGNRR AKVNDFPYCF HLLSGEKEQV SSEALEACRI ACNKYIAKKA GKDSFHLRI RVHPFHVIRI NKMLSCAGAD RLQTGMRGAW GKPQGTVARV SIGQPLLSVR CRASAKDYVK DALRRAKFKI P GRQAIVES RNWGFTEFTK EEYEDLRERG ELQYDGNNAH RISRKGPLNL KL

UniProtKB: Ribosomal protein L10

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Macromolecule #36: Ribosomal protein L11

MacromoleculeName: Ribosomal protein L11 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 19.767684 KDa
SequenceString:
MSQESENPMR RIRIEKLVLN ICIGGEGDAL TKAAKVLQQL TDQEPTFSKS RLTIRGFGIR RGQKISTHVT VRGEKAREII ERGLRVKNF ELPYSCFSTT GNFGFGITEH IDLGIKYDPS MGIYGMDFYV VLSRPGFRVN KRRAQQQHVG ASHKITPDEA R AWVEREFE VVYTK

UniProtKB: Ribosomal protein L11

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Macromolecule #37: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 26.640346 KDa
SequenceString: MASYASFILR LIHFNLNFVT AFCPLPRAMG RNVAFSNMRR YKCTNKGPNL IKNWFNQPAR KIRRRQTRLQ KAKAIFPCPL QKLRPSVTR CSQKFNLTQR IGRGFTLREL QGVGLSAGKA RQIGIAVDPR RKNKSEESVA RNVARLKEYL SKVTIFSKQA K PEEIQQAI ...String:
MASYASFILR LIHFNLNFVT AFCPLPRAMG RNVAFSNMRR YKCTNKGPNL IKNWFNQPAR KIRRRQTRLQ KAKAIFPCPL QKLRPSVTR CSQKFNLTQR IGRGFTLREL QGVGLSAGKA RQIGIAVDPR RKNKSEESVA RNVARLKEYL SKVTIFSKQA K PEEIQQAI QHQGVVMPVV NPKPVITTGS IAEAKKEVPE NLFMYLRNAR TARNRARLAK RDAEREAAAK TKGGKK

UniProtKB: 60S ribosomal protein L13

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Macromolecule #38: Ribosomal protein L14

MacromoleculeName: Ribosomal protein L14 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.785472 KDa
SequenceString:
MSYHRFVEAG RLVYLGDGPD KGKLAVIVDI LSTAAVLIDG PTTGVKRQVV SLRWAMLTDQ VINISEKPTT EQLKKEIEAS PAIDTFMNS VWGKKIQQQR LRAKATFEDM GKIAKLRRER KMAMKRIAMS IK

UniProtKB: Ribosomal protein L14

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Macromolecule #39: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 24.35373 KDa
SequenceString: MGAYKYVQEL WRKKQSEALH YLFRLRTWEY RQLNAIKRCS HPTRPEKARR LGYKAKRGYV VYRVRVRRGG RKRQCVHGIS YGKPRNIGI VGLKPSRSLR ALAEQKVGRR LPSLRVLNSY WVAQDGSYKW YEVILIDPFH PWIRNDPKIN WICNPVMKRR E CRGLTSAG ...String:
MGAYKYVQEL WRKKQSEALH YLFRLRTWEY RQLNAIKRCS HPTRPEKARR LGYKAKRGYV VYRVRVRRGG RKRQCVHGIS YGKPRNIGI VGLKPSRSLR ALAEQKVGRR LPSLRVLNSY WVAQDGSYKW YEVILIDPFH PWIRNDPKIN WICNPVMKRR E CRGLTSAG RKARGLHNKG IGAMKLRPSK RAAWKRNHTR SLRRYR

UniProtKB: Ribosomal protein L15

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Macromolecule #40: Ribosomal protein L13a

MacromoleculeName: Ribosomal protein L13a / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 22.921914 KDa
SequenceString: MSRIVIDCKG HILGRLSAIV AKELLHGHEV ALVRCEKAII SGAFRDVKAH YLKKMHKRTN FNHLRGPFHF RAPAEIVRRT IRGMIPYKT VRGSDAMSRL RVYDGIPPCC ARYKRVVCPS AMAYLCLRPE RRRCEVGRVA HEIGWQHAAD VERYEKRRLE E CDKWYQDK ...String:
MSRIVIDCKG HILGRLSAIV AKELLHGHEV ALVRCEKAII SGAFRDVKAH YLKKMHKRTN FNHLRGPFHF RAPAEIVRRT IRGMIPYKT VRGSDAMSRL RVYDGIPPCC ARYKRVVCPS AMAYLCLRPE RRRCEVGRVA HEIGWQHAAD VERYEKRRLE E CDKWYQDK IQIDKKIAEA AEKDAELQRI NAELAKYGY

UniProtKB: Ribosomal protein L13a

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Macromolecule #41: Ribosomal protein L17

MacromoleculeName: Ribosomal protein L17 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 18.722785 KDa
SequenceString:
MVKYAFKSDN LLVGKSRRDG VKASFKNTYS TCRAIKGMGV KRAFAYLDNV LNHKEIVPFF KFYKGVGRHS QARGSGTDKG RWPEKSVVV VKDILKQAVS NAKHAHKKEE DELYVISYCV NRARGRRRRT FRAHGRISKY ESQPCHVELI VGPKPLDVEK A KALQ

UniProtKB: Ribosomal protein L17

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Macromolecule #42: Ribosomal protein L18

MacromoleculeName: Ribosomal protein L18 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 20.207768 KDa
SequenceString:
MGIDLKTGIK KRKVERKEPK SPNPYIRLLH NAFKVVYENT GEDFTKVLTK RLCLSKTNRP VLSVSRLAHL MHKREDKIAV VASTVTNDE RLVTIPRMTI CALKFSATAR SRIEKAGGRC MSFDELLAEK PTGDNCVLLQ GRRSCRAVCK KFGSPGRPNS H VKPKGEHK GRRGARGRHS W

UniProtKB: Ribosomal protein L18

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Macromolecule #43: Ribosomal protein L19

MacromoleculeName: Ribosomal protein L19 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 23.199238 KDa
SequenceString: MANLRLQKRL AADVLKCGKR RVRFNPAHEK YIEQASSREA IRSLIESKII AKLDIKGSSR GRIRARHAAK RLGRHSGHGK RRGTREARM PSKTLWIQRQ RVLRRLLSRY RDDEKIDRHL YETLYAKAKG NMFKNKRVLL EHIVGMQKEQ ELEKKKKEEL E QRRKKAEQ ...String:
MANLRLQKRL AADVLKCGKR RVRFNPAHEK YIEQASSREA IRSLIESKII AKLDIKGSSR GRIRARHAAK RLGRHSGHGK RRGTREARM PSKTLWIQRQ RVLRRLLSRY RDDEKIDRHL YETLYAKAKG NMFKNKRVLL EHIVGMQKEQ ELEKKKKEEL E QRRKKAEQ RCKERAEKVA ANRLAFAQTG IFTEKTNK

UniProtKB: Ribosomal protein L19

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Macromolecule #44: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 20.624902 KDa
SequenceString:
MMQKYIVYGR RLPSEKFPEP EVFRMTIYAP NEIVAKSRFW YHLKSLKKIK RTHGEILDCQ HIEENGDFVK NFGVLLRYRS RVGQHNMYR EVRETTSARA MDKVYSEMAG QCRARYHDIQ IINVKEVADE DVRREKVAMF TQKGVRFPHP CPYVHVKRAA R TARFQDRA PHVPQ

UniProtKB: 60S ribosomal protein L18a

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Macromolecule #45: Ribosomal protein L21

MacromoleculeName: Ribosomal protein L21 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 18.296393 KDa
SequenceString:
MTHSQGYRCK TRYCFARKFR RHGMANLTTY NRVYKLGQYV NIKVNGAYHK GMPHRYYNGR TGTIFNIGPR ALGVQIQKRV RGRIMLKRI NVRLEHVHPS DCRKNHLERV AANDAARRLA KETGQPVPCL KRQNALPRAA HVVSIASAID ITPLKYDGVF

UniProtKB: Ribosomal protein L21

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Macromolecule #46: Ribosomal protein eL22

MacromoleculeName: Ribosomal protein eL22 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 19.942402 KDa
SequenceString:
MEGQWILFPD QIVNHLCRHA DHRLQARVSS KSNLKSNYAY YFSPSSEMRP RIKKCVKKEQ GNIRFAVDCS AGKDLELNLN EFADYVRTH FKINNRKHGV AEKVACSVEG DSLVIQTTGY EFAKRYVKYL TKRFLYQDYQ GVFRVLSTDK ETYTLKPYTI E DDDDEAGT DDE

UniProtKB: Large ribosomal subunit protein eL22

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Macromolecule #47: Ribosomal protein L23

MacromoleculeName: Ribosomal protein L23 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.45004 KDa
SequenceString:
MASKRGRSGG IGNKFRISCG LPVGAVINCA DNSGAKSLNM IAVKNWKGHL NRFPKASVGD MVLITVGRDG KPELRRKVTP AVVVRQRKV WRRKDGVWIY FQDNAGVIIN PKGELKGSSI TGPVAKEAAD RWPKISTTAE CVH

UniProtKB: Ribosomal protein L23

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Macromolecule #48: Ribosomal protein L23A

MacromoleculeName: Ribosomal protein L23A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 16.053919 KDa
SequenceString:
MSEKAQALKA QKAILHGTRV EAKKIRTNVH FFRPKTLVHK KAPLYPRNPI KKGSWDDPCD LLRYPIKTDE NTTQIEKNNT IVFIVDRRA SKPEIRKAFE TVFETKVARV NTLITPLGLK KAFIRLAPDV QAMDVASKMG LA

UniProtKB: Ribosomal protein L23A

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Macromolecule #49: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.621398 KDa
SequenceString:
MKLNSAVTAS RRKCRKAYFT ANAETRAKMM SSRLSKELRA EHKIKTMPIR RGDIVEIFTG GHKGTGKVVE VRRRDYKICV EGINQKARN PEAKPVPYPI HPSNCIIKEL YMNGSRYRAI KRRQERNAER LARIGK

UniProtKB: Ribosomal protein L26

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Macromolecule #50: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.374895 KDa
SequenceString:
MSQPIIKPGL VCIVLQGRHA GKKAVIVEAL PHNERGSYAI VAGIAALPRR ITNSMSDEEA VCRGRVRSFV KCMNVNHLMP TSCRLENNY KDFISTQTVA DVTKRREIIE TVNKDFQRRI LEHRDTWFFT KLPIDA

UniProtKB: Ribosomal protein L27

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Macromolecule #51: Ribosomal protein L27a

MacromoleculeName: Ribosomal protein L27a / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 17.166957 KDa
SequenceString:
MPTRIRKSRK RRGHRQMGYG RVGGHRKHPS GRGKAGGLQH KRTSFDKYHP GYFGKLGMRN YHVRRNIEYN PTINIDKIWS LIPEEQRDG FLQRKSTTEA PVVDVTRFGF FKVLGRGSLP QCPIIIKARY FSSDAEKKLR EVGGVPLLVP

UniProtKB: Ribosomal protein L27a

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Macromolecule #52: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 7.363574 KDa
SequenceString:
MAKLKNHTSK NQNRKDHRNG IKKPKKSAYT SHKGMCPKYL RNLRRSRAND PRQSLRPNLN KE

UniProtKB: 60S ribosomal protein L29

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Macromolecule #53: Ribosomal protein L30

MacromoleculeName: Ribosomal protein L30 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 11.698598 KDa
SequenceString:
MSTKVATKKA QDSISLTLTL VVKSGKYTLG TDQAIRNIRD GKAQLVFMAN NCPPLVKSQV DYLCHLSKTP VRVFAGSSRE LGIAVGRQF NVSIMTILEA GDADLTSILG

UniProtKB: Ribosomal protein L30

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Macromolecule #54: Ribosomal protein L31B

MacromoleculeName: Ribosomal protein L31B / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 12.184064 KDa
SequenceString:
MGIVYEHTIR LNKAVRGKPS KKAAPIAIRA IRAQVEKLAK VEDVRLDPSV NVFVWSRGIR HVPRRIRLEV RLEGDDEHKF AYVINKDVE NFKHLTTEKK GSEDGSE

UniProtKB: Ribosomal protein L31B

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Macromolecule #55: Ribosomal protein L32

MacromoleculeName: Ribosomal protein L32 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.985983 KDa
SequenceString:
MAGEMIHPLP HKIIIKKRKN KFIRFQSERF VRVKPSWRKP RGIDSPMRHQ YRGQRPMVSI GYGSDKETRF MLPCGFFKHR ISTVHELKT LFMNNKTHAA QIAHGVSARS RKQILEEAKK MGVRVLNADA KLRKEEL

UniProtKB: Ribosomal protein L32

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Macromolecule #56: Ribosomal protein L35a

MacromoleculeName: Ribosomal protein L35a / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 13.913122 KDa
SequenceString:
MEEIKGKTFL PAIFVGYQRS RAKQRENVAL VKVSGIYTRA ETDKVLGRLI AIIHSKSGKP IFGKICRPHG NSGMVRARFY TNLPGQLMG HKVRVFYDNI DVGDRATPKY ERLYGNPNAT VTYE

UniProtKB: Ribosomal protein L35a

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Macromolecule #57: Ribosomal protein L34

MacromoleculeName: Ribosomal protein L34 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 13.528753 KDa
SequenceString:
MADCRVTCRF HSTYRTRSKI VRYKRTPGNR LTSLRVKKLP NAPHCAETGK QIHGIPRVIP QHLTRQQRKV SRPYGGKLCG SALRAHILE SFLNEENQAL REIAAQTAKT AKQTAGKGHA K

UniProtKB: Large ribosomal subunit protein eL34

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Macromolecule #58: Ribosomal protein L35

MacromoleculeName: Ribosomal protein L35 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.292209 KDa
SequenceString:
MPGLKAKDLV GLSQEDLQRK LADLKRELLS LRTMKATAGP VPERIARFRV CKKDVARVLT VINQKARDEA RAAFEGAEHI PKTFRPRLT HAMRCQLTEK QKRLLPSKLM KRKLQFPKLK YAVKV

UniProtKB: Ribosomal protein L35

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Macromolecule #59: Ribosomal protein L36-1

MacromoleculeName: Ribosomal protein L36-1 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 10.586708 KDa
SequenceString:
MPGKVFNLKK GGAVVRIVRK KEERKTKPHQ EFVKSIIQEC TGMAPYEMHI IELLRMNKDR HALRYAKKRL GNIKRAKAKR DQLSVYARN I

UniProtKB: Ribosomal protein L36-1

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Macromolecule #60: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 10.329022 KDa
SequenceString:
MSKGTASFGK RHTRIHGSCR RCGKHSFHLQ KHECASCGYP SAKMRRYNWS YKSLRRRTQG TGSMSHMRKV YRAYNSGTLK ANARKYLAK

UniProtKB: Ribosomal protein L37

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Macromolecule #61: Ribosomal L38e

MacromoleculeName: Ribosomal L38e / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 8.605145 KDa
SequenceString:
MPNTITEENL MKMIADGRGK KLVVKSCSKG RLQGKVKLKL RTAKYLYTLT VPNADDAKSI VKGCKDKVEV QYVNDSE

UniProtKB: Ribosomal L38e

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Macromolecule #62: Ribosomal protein L39

MacromoleculeName: Ribosomal protein L39 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 6.353656 KDa
SequenceString:
MTANKTLSRK LRLAKKTKTN KNIPRWVIAK DHLKKTWNYK RHHWRRSHLK L

UniProtKB: Ribosomal protein L39

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Macromolecule #63: Ubiquitin/Ribosomal protein L40e

MacromoleculeName: Ubiquitin/Ribosomal protein L40e / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.343675 KDa
SequenceString:
MQLIVRSLDR TVALTASPAD SLTSIRQRLL AVYSGHVVDS QRFVFAGRTL DEAKTLGDYS IGESSVLDLV PRLFGGVMEP TLINLAREY NQNMMICRHC YARNPLRATN CRRCRGTNLR KKKLSKYA

UniProtKB: 50S ribosomal protein L40e

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Macromolecule #64: Ribosomal protein L44

MacromoleculeName: Ribosomal protein L44 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 12.311678 KDa
SequenceString:
MVTYPAERRT FCKREGKHTV HKASLYKKGK DSLLAQGKRR YDRKQRGFGG QTKPIFRRKA KTTKKQVIRL TCSSCKCQTF QVLKRCHRL EISRLGATKK SKGSVVF

UniProtKB: Ribosomal protein L44

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Macromolecule #65: Ribosomal protein L37a

MacromoleculeName: Ribosomal protein L37a / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 10.508366 KDa
SequenceString:
MAKRTKKVGL TGKYGTRYGR AARKQLLKIE TSQKAKYTCP FCAKDKVRRT ACGIWTCRSC SKTMAGGAYS LHTEPANAAR ATIRRLRLA REDAQ

UniProtKB: Ribosomal protein L37a

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Macromolecule #67: Ribosomal protein L24A

MacromoleculeName: Ribosomal protein L24A / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 12.005191 KDa
SequenceString:
MVTTNQCSYS GRKILPGYGK RMSRHDKVLL IFLNRKASVH YLNKWNPRKI RWTLASRRAR GKEVIVKARE RVTRKAVVSK RNFLSIDDS KLEALKSKYS RVN

UniProtKB: Ribosomal protein L24A

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Macromolecule #69: Ribosomal protein S29A

MacromoleculeName: Ribosomal protein S29A / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.49978 KDa
SequenceString:
MRSALDTGVA ADRIGRPQTF AELRVDKVDD IGTQRHIEHL GPRKLRNLGS CGAEDLDSRV HPSTIFWPTE ADNINLASGV PKKGPSMRE TEFCTHIAHK RECRICGNYN SVYRKYGLFI CRRCFKENAG KIGFVKLH

UniProtKB: Ribosomal protein S29A

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Macromolecule #70: Ribosomal protein S5

MacromoleculeName: Ribosomal protein S5 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 21.058275 KDa
SequenceString:
MSLEVALFNK WPLTDVKCQD ESLEAYISID NHSFVPHSAG RWSAKRFRKA KCPVVERLTN SLMMHGRNSG KKLMAMKTVG EAFELINLY TGKNPVQVLV DAVANSGPRE DSCRIGKGGQ VRRQSVDVSP LRRVNIGIYN IATGARKAAF RKVRPFAECL A EEIMNAAA GADKSYAISQ RNSVERIAVS NR

UniProtKB: Ribosomal protein S5

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Macromolecule #71: Ribosomal protein S24

MacromoleculeName: Ribosomal protein S24 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.872646 KDa
SequenceString:
MPEITVKVRK VLNNPLLQRQ QCVVDVLHPG CTYESKEAIK AKVAQQLKVA DQKNIVLYGF KTSFGGGHTV GFCNAYQNMD ALMKYEPGF RKIRCGLIEA PKPVSRKQLK NLKNRRLKKR GTEKATVTLG AKK

UniProtKB: Ribosomal protein S24

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Macromolecule #72: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 28.084123 KDa
SequenceString: MPKGSFCKVN ISNPVTGANK SLMIEDFRKS QTIHGLRMAS EFDGAALGEE YAGCTFRITG GNDAQGFPMY QGVLKSQRAR LLLRQGAKC YLHRRNGERK RKSIRGCIVG PDLHALSVVL VDAGDKTLPG LTDVVVPKRL VPKRASKIRR LFGLPTTKVD P KDQMLVCE ...String:
MPKGSFCKVN ISNPVTGANK SLMIEDFRKS QTIHGLRMAS EFDGAALGEE YAGCTFRITG GNDAQGFPMY QGVLKSQRAR LLLRQGAKC YLHRRNGERK RKSIRGCIVG PDLHALSVVL VDAGDKTLPG LTDVVVPKRL VPKRASKIRR LFGLPTTKVD P KDQMLVCE LIKELGHEVT LKNGEKKLKY PKVQRVVTDE KYARKQRVIA EKKEIMRKSL QQLEEYKALL SRMGKQHNIR IY KPAKRTV E

UniProtKB: 40S ribosomal protein S6

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Macromolecule #74: 40S ribosomal protein SA

MacromoleculeName: 40S ribosomal protein SA / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 28.101803 KDa
SequenceString: MSTEKTSQAS KEYQLKEADV KKMLVATTHL GVRNIDRRMQ FYIFDRQKDG TFVFNLQKVW AKIVFAARIL VTIDDPAEIA VVANRPDAQ RAILKFCKYT HATAFPGRFI PGNFTNRMNP NYCEPRLLLV NDPVVDRQAI LEASYVNIPT ISLCNSDANL K FIDVAIPC ...String:
MSTEKTSQAS KEYQLKEADV KKMLVATTHL GVRNIDRRMQ FYIFDRQKDG TFVFNLQKVW AKIVFAARIL VTIDDPAEIA VVANRPDAQ RAILKFCKYT HATAFPGRFI PGNFTNRMNP NYCEPRLLLV NDPVVDRQAI LEASYVNIPT ISLCNSDANL K FIDVAIPC NNKTPMSIGL IYWLLAREVL RLKGSISRTE EWDVKPDLFV ALPEEIPDEE ESEDFYDDDE EEDEEFSAGN GN LFDEY

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #25: rRNA 28S

MacromoleculeName: rRNA 28S / type: rna / ID: 25 / Number of copies: 1
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 878.2385 KDa
SequenceString: GCGCGGCCCG AGGCGGCGGG GGCGACGGGC GGAACUUAAG CAUAUCAG(OMU)A CGCCCCGGAG GAGAAACCAA CCGGGA UUC CCCGUAGCGG CGAGCGACGC GGGAGGAGCC CGCCCCGAAG GCGCGCUGUG GGGCGCAGGC GCAGGCCCGC CGCGAGG GG GCCCGAGGGC ...String:
GCGCGGCCCG AGGCGGCGGG GGCGACGGGC GGAACUUAAG CAUAUCAG(OMU)A CGCCCCGGAG GAGAAACCAA CCGGGA UUC CCCGUAGCGG CGAGCGACGC GGGAGGAGCC CGCCCCGAAG GCGCGCUGUG GGGCGCAGGC GCAGGCCCGC CGCGAGG GG GCCCGAGGGC CCCGCCCGAG AGGGUGCAAG CCCCGUACGG CGGCCGCCGG GCCUGCGCGG CGAGUAGCGC UGCUUGAG C GUGCAGCGCG AAGGGAGGCG CGGCCCUUCC AAGGCUAAAU ACGCCCCGGG ACCGAUAGCG GACCAAGUAG CGCGA (OMG)CGA ACGGUGAAAA GGACGCCCUG CGGCCGCUCA AAAGACCUGA ACCCGGCCGG CCGCCGGCCC GCCGGCCCC (OMG)UCUCGA(A2M)AC (A2M)CGGACCGAG GAGCCACGCG CCGCGGCGAG CCCGAGGGAG CCCCCGCGGC GGAGCGAG C GCGAGACGCC CCGGGCCCGC CGCGCCCCUG CGGGCGUGCG CGGGCCGAGC CGCGGCGCGU GGGCCCGA(A2M)A GGCGG UGAU CUAUGCCCGG CGAGGGCGAG GCCGGGCGAA AGCCUGGUGG AGGCCCGCCG CGGUGCUGAC GCGCAGAUCG CUCGUC GGA GCCGGGCAUG G(OMG)GGCGAAAG ACUCAUCGAA CCGCCUGGUA GCUGGUUGCC UCCGAAAUGU CUCCCAGGAC AGC CGCCGC CCCGCAGUUG CGGCCCGUAG AGCGCUGGCC GGCGGGAGCG GGGGGCCUGC CCCUCGCCCG CCCCCCAAAC UCCG AAGGG CCGCGCCGCC CCGCCGCUGG CCUGGGCGGG GCGGGCGAAU GCGGGCGGCG CGUGGGCCCC UCCUGGUAAG CAGGA CGGG CGAGGCGGGA CGAUCCGGAC GCCGGGCCAG GGUGCGCCGC CGGGGCCCGC GGAACGGCGU CGGCCGGUCC CGACAG CUG GAAGGUGGCC CCAGAAGUCG GCAUCCUCCA GGGAGUGUGU AACAACCCAC CAGCCGAAUC GGCCGGCCCG GAAAAUG GA GCGCGCCGGA GCCCCGGACC CGCGCCCGGC CGCCGCGCGC GGCGGGUAGG AGGCCGCAGA GGCCCCGGGG GCGAAGGC G GCGCGCAGGC CCCGCCGGAC CGGCCUCUGG UGCA(OMG)AUCUC GGCAGCAGUA GCCGCUACUC CGCGCCCCGG AGGAC UGAG GGGGAGACGG GUUCCGCGGC GCCUGCAUCU GGCCGCGG(OMG)U GACUCGGGCC UAAGCGGCGG GUGAAGACCG GG AAGGGGC GUGCCCGCCC GUCGAACGGG GAGCCGGCGG AGACUCCGGC AGGCGCGGCC CCCGCGGAGA CGCCCGCCCC CCG GCGACG CGCACGGGGA CCGCGGCGGG CGGCGCCCCG GCCCGCGAAC GCCCCGCAGC CCCCGGACGC CUUGCGCGGA GAGG GGGGC CCGGGGGCGG ACCCCGCGCG UCCCCGGCCG CCCCUGAAAA GCCGGGGGGC GCCGGCCGCG CGCCGUACCG ACCGC AGCA GGACUCCGGG GUCAGCAGCC UCUAGCGCGG GAGCGAAC(OMG)C GGCUCAGGGA AGUCGGCAAG CCGGCUCCGU AA CCUCGGG AAAAGGAGUG GCUCUGACGG CGCGCCGGGU CAGAACUGGA ACGGACGCGG GGAUCCCGAC UGUUUACUAG AAA CACAGC GUCGCGAGGG CCGCACCCGG CGCUGGCGCG ACGUGAUUUC UGCC(OMC)AGUGC CACGACCGUC ACCGUGAAGC GAUCCGCCG AAGCCCUGGU AAACGGCGGG AGUAACUAUG ACUCUCUUAA GGUAGC(5MC)AA(A2M) UGCCUC(OMG)UC GGGCAAUUUC CGACGUGCAU GAAUGGACCA ACGAGGAUCC CACUGU(OMC)CCG AGCCGCGCCU CCGCGAGCCU CCAGCC UCG GGAACGGGCG AGGGCCGGCC AGCGG(OMG)GCAA GAAGACCCU(OMU) (OMU)UGAGCUUGA C(OMU)CCAGCCCG G GCCUGUGG GGCGGGGCGG CCGGCGCAGC GCACAGGGGA GGCCGCGCCC CUGAGACACC CUGACGGCCG CCGCCGCCCC GC UCACCCG GUCGCGCGGG GACCCGCCCG GGCGGGGAGU UCGGCUG(OMG)GG CGGCGCGCCU GCUACACCGG ACCGCAGGC (OMG)UCCCACGGC GGGCUCAGCG AGGACGGAGA CCUCCCGCGG AGCAGAAGGG CACAAGCCCG CCCGACCCGC GCCCCC CGU GCCGGCGCGG GCCGCGAAAG CGGGGCCUAC CGAUCCUUCG CCGCCCCGGC CGCGGGCGCG GAGGUGGCAG AAAAGUU AC CACAG(OMG)GAUA ACUGGCUUGU GGCCGCCGAG CGCCCGCAGC GACGCGGCUU UUUGAUCCUU (5MC)GAUGUCGGC UCUUCCUAC CGUCCGCGCG CACCGGCGCG GAAGCGUCGG AUUGUUCACC CGUUCAAGGG AUCGUGAGCU GGGUUUAGA (OMC)CGUCGUGAG ACAGGUUAGU UUUACCCUAC UGGCCCCGGG GCCAGAGCAC GGCGGGCCAG UACGAGAGGA ACGCCC GCC GCGGGCGCCC AGCCCCGCGG UUGCCCGCCG GGGCAGGACC GCGCGCCCGG GCCCGGGGGC CUGGCGCUGC CGCCUCU AA AGCGCCACCC CCCCCUCCGG CCCCGCCGGG CCCGCGCCCC AGCCCCGUGC CCCCUGCCCG AGGCGGCCCC CGCCCGGG A GGACCACCCG GCGCGGCGCC CCUGUACGGC GCAGGGCCUG CGAUCGCGUU CGCCCGGGGG GCGCGCCGGG CAGGCGCGC GGCCCACUUG CU

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Macromolecule #26: rRNA 5S

MacromoleculeName: rRNA 5S / type: rna / ID: 26 / Number of copies: 1
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 38.671039 KDa
SequenceString:
CAGUUCGGCC AUCCUACGGC GGAAACUCCU UAACUCGUUC CGAAUUAAGA AGAGAAGCGC CGUCAGGCCC CGCCAGUACU GCGAUCGGA GACGUCGUGG GAACACGGGG UGCCGAACCU U

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Macromolecule #27: rRNA 5.8S

MacromoleculeName: rRNA 5.8S / type: rna / ID: 27 / Number of copies: 1
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 44.971965 KDa
SequenceString:
GAACGCCCCG CCGGCGGAUG CCUCGGCCCG GGCGGCGACG AAGAGCGCGG CGGAGCGCGA GACGCGGUGC GGACCCGCCC GCCCCGAGA AGCACCGACC CUCGAACGCA GCGCGCCCCG GCGCCGCCGC CUC(OMG)GCGCCC

GENBANK: GENBANK: X52949.1

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Macromolecule #66: E-site tRNA

MacromoleculeName: E-site tRNA / type: rna / ID: 66 / Number of copies: 1
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 4.459755 KDa
SequenceString:
AGCGGCCGCC ACCA

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Macromolecule #68: P-site tRNA

MacromoleculeName: P-site tRNA / type: rna / ID: 68 / Number of copies: 1
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 894.612 Da
SequenceString:
CCA

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Macromolecule #73: rRNA 18S

MacromoleculeName: rRNA 18S / type: rna / ID: 73 / Number of copies: 1
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote)
Molecular weightTheoretical: 471.124531 KDa
SequenceString: CAUCCGGUCG AUCCUGCCGG AGCGCGACGC UCUCCCCAAG GACGAAGCCA UGCAUGCCCG CUCACCCGGG ACGCGGCGGA CGGCUC(A2M)GG ACAACGGUUG CACC(OMC)CCCGU GGCGGUCCCU GCUAGCCGGA CACCGCUGGC AACCCGGCGC CAA GACGUG ...String:
CAUCCGGUCG AUCCUGCCGG AGCGCGACGC UCUCCCCAAG GACGAAGCCA UGCAUGCCCG CUCACCCGGG ACGCGGCGGA CGGCUC(A2M)GG ACAACGGUUG CACC(OMC)CCCGU GGCGGUCCCU GCUAGCCGGA CACCGCUGGC AACCCGGCGC CAA GACGUG CGCGCAAGGG CGGGCGCCCG CGGGCGAGCA GCGUGACGCA GCGACGGCCC GCCCGGGCUU CCGGGGCAUC ACCC GGUCG GCGCGGUCGC GGCGCGCCGA GGGCCCGACG CCUGGCGGAG AAUCAGGGUU CGACUCCGGA GAGCGGGCCU GCGAG ACGG CCCGCACAUC CAAGGACGGC AGCAGGCGCG G(A2M)ACUUGCCC AAUGCGCGGC GCGC(OMG)GCGGC GCGACGGG G UGCGCGCGAG UCAGGCGGGC CCACCCCGAC CGCCGCGGAG CGCCAGGGCA AGGUCUGGUG CCAGCAGCCG CGGUAAUUC CAGCUCGGCG AGCGUCGCGC GGCGCUGCUG CAGUUGAAAC GCCCGUAGUU GGCCCCCCGC CGCCACGAGG AAACGGGAGC GCUCCAGGC AGGCCCGUUG GACCCGCCGC GUGGGACCGC GCAGCGGGCC CGGCGCGCCG CGGCAGCCCC GAGGAGAGCG G GCGGGGGC ACCGGUACCG GCCGGGGACG GGUGAAACAG GAUGAUCCCG CCGAGACCGC CGGCCGCGCA GGCGCCUGCC AA GACCGCC UCUGUCAAUC AAGGGCGAAG GCCGGGGGCU AGAAGGCGAU CAGACACCAC CGUAUUCCCG GCCGUAAACG GUG CCGCCC CGCGGCCGGC GCGCGCGUCC CGCCGGCCGC CCAGGGAAAC CGGGAGGCUC CGGGCUCUGG GGGGAGUAUG GCCG CAA(OMG)G CUGAAACUUG AAGGCAUUGA CGGAGGGGUA CCACCAGACG UGGAGUCUGC GGCUCAAUCU GAC(C4J)CAA CG CGCGCACCUC ACCAGGCCCG GACGCGCGGA GGACCGACAG CCGGGCGCGC UUUCGCGAUC GCGCGGGCGG UG(OMG)U GCAUG GCCGCUCCCA GCCCGUG(OMG)CG CGAGCCGUCU GCUCCAUUGC GACAACGAGC GAGACCCCGG CCGCGGGCGC C GCGGGACG GCCCGCGCGA GCGGGAGGAC GGCGGGGCGA UAGCAGGUCU GUGAUGCCCU CAGACGCCCU GGGCCGCACG CG CGCUACA CUGGCGGGGC CAGCCGGCGC CCGCGAGGAC GCGCGGAGCC CCCGCCGUGG CCGGGACCGC GGGCUGGAAC GCC CCCGCG CACCAG(7MG)AAU GUCUUGUAGG CGCCCGCCCC CACCGCGCGC CGGACGCGUC CCUGCCCCU(OMU) GUACAC ACC G(4OC)CCGUCGCU CCUACCGACU GGGCGCUGCG GCGAGCGCCC CGGACGCGGG AAGGGCCGCG AGCCCC(M7A)GC GCCUGGAGGA AGGAGAAGUC GUAACAAGGU AUC(4AC)GUAGGU G(MA6)(MA6)CCUGCGG AUGGAUCCCU

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Macromolecule #75: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 75 / Number of copies: 455 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #76: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 76 / Number of copies: 101 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 5567 / Average electron dose: 1.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.75 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 91058
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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