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TitleCryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
Journal, issue, pagesNucleic Acids Res, Vol. 50, Issue 3, Page 1770-1782, Year 2022
Publish dateFeb 22, 2022
AuthorsDisha-Gajanan Hiregange / Andre Rivalta / Tanaya Bose / Elinor Breiner-Goldstein / Sarit Samiya / Giuseppe Cimicata / Liudmila Kulakova / Ella Zimmerman / Anat Bashan / Osnat Herzberg / Ada Yonath /
PubMed AbstractGiardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs is spreading, new strategies for combating ...Giardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs is spreading, new strategies for combating giardiasis need to be developed. The G. lamblia ribosome may provide a promising therapeutic target due to its distinct sequence differences from ribosomes of most eukaryotes and prokaryotes. Here, we report the cryo-electron microscopy structure of the G. lamblia (WB strain) ribosome determined at 2.75 Å resolution. The ribosomal RNA is the shortest known among eukaryotes, and lacks nearly all the eukaryote-specific ribosomal RNA expansion segments. In contrast, the ribosomal proteins are typically eukaryotic with some species-specific insertions/extensions. Most typical inter-subunit bridges are maintained except for one missing contact site. Unique structural features are located mainly at the ribosome's periphery. These may be exploited as target sites for the design of new compounds that inhibit selectively the parasite's ribosomal activity.
External linksNucleic Acids Res / PubMed:35100413 / PubMed Central
MethodsEM (single particle)
Resolution2.75 - 2.85 Å
Structure data

13680

7pwf

EMDB-13680, PDB-7pwf:
Cryo-EM structure of small subunit of Giardia lamblia ribosome at 2.9 A resolution
Method: EM (single particle) / Resolution: 2.85 Å

13681

7pwg

EMDB-13681, PDB-7pwg:
Cryo-EM structure of large subunit of Giardia lamblia ribosome at 2.7 A resolution
Method: EM (single particle) / Resolution: 2.75 Å

13683

7pwo

EMDB-13683, PDB-7pwo:
Cryo-EM structure of Giardia lamblia ribosome at 2.75 A resolution
Method: EM (single particle) / Resolution: 2.75 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

Source
  • giardia lamblia atcc 50803 (eukaryote)
  • Giardia intestinalis (strain ATCC 50803 / WB clone C6) (eukaryote)
KeywordsRIBOSOME / giardia / parasite

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