[English] 日本語
Yorodumi
- EMDB-13681: Cryo-EM structure of large subunit of Giardia lamblia ribosome at... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-13681
TitleCryo-EM structure of large subunit of Giardia lamblia ribosome at 2.7 A resolution
Map data
Sample
  • Complex: Giardia lamblia ribosome
    • RNA: x 5 types
    • Protein or peptide: x 39 types
  • Ligand: x 4 types
Function / homology
Function and homology information


maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / protein tag activity / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation ...maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / protein tag activity / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / metal ion binding
Similarity search - Function
Ribosomal protein L40e, archaeal / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily ...Ribosomal protein L40e, archaeal / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L30/YlxQ / 60S ribosomal protein L35 / Ribosomal protein L7A/L8 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L37ae / Ribosomal protein L31e, conserved site / Ribosomal L37ae protein family / Ribosomal protein L31e signature. / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L39e / Ribosomal protein L14e domain / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / Ribosomal protein L14 / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L14 / Ribosomal protein L14, KOW motif / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L31e / Ribosomal protein L15e, conserved site / Ribosomal protein L31e domain superfamily / Ribosomal protein L31e / Ribosomal protein L15e signature. / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal_L15e
Similarity search - Domain/homology
Ribosomal protein L39 / Ribosomal protein L29 / Ribosomal protein L27 / Ribosomal protein L21 / Ribosomal L38e / Ribosomal protein L14 / Ribosomal protein L18 / Ribosomal protein L30 / 60S ribosomal protein L13 / Ribosomal protein L4 ...Ribosomal protein L39 / Ribosomal protein L29 / Ribosomal protein L27 / Ribosomal protein L21 / Ribosomal L38e / Ribosomal protein L14 / Ribosomal protein L18 / Ribosomal protein L30 / 60S ribosomal protein L13 / Ribosomal protein L4 / Ribosomal protein L15 / Ribosomal protein L17 / Ribosomal protein L26 / Ribosomal protein L19 / Ribosomal protein L11 / Ribosomal protein L35a / 60S ribosomal protein L7a / Ribosomal protein L37 / Ribosomal protein L10 / Ribosomal protein L32 / Large ribosomal subunit protein eL34 / Ribosomal protein L31B / Ribosomal protein L23A / Ribosomal protein L37a / Ribosomal protein L6 / Ribosomal protein L44 / Ribosomal protein L24A / Ribosomal protein L7 / Ribosomal protein L3 / Ribosomal protein L23 / 60S ribosomal protein L18a / Ribosomal protein L13a / Large ribosomal subunit protein eL22 / 50S ribosomal protein L40e / Ribosomal protein L36-1 / 60S ribosomal protein L29 / Ribosomal protein L27a / Ribosomal protein L2 / Ribosomal protein L5
Similarity search - Component
Biological speciesGiardia intestinalis (strain ATCC 50803 / WB clone C6) (eukaryote) / Giardia lamblia (eukaryote) / Giardia lamblia ATCC 50803 (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.75 Å
AuthorsHiregange DG / Rivalta A / Bose T / Breiner-Goldstein E / Samiya S / Cimicata G / Kulakova L / Zimmerman E / Bashan A / Herzberg O / Yonath A
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI119788 United States
CitationJournal: Nucleic Acids Res / Year: 2022
Title: Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
Authors: Disha-Gajanan Hiregange / Andre Rivalta / Tanaya Bose / Elinor Breiner-Goldstein / Sarit Samiya / Giuseppe Cimicata / Liudmila Kulakova / Ella Zimmerman / Anat Bashan / Osnat Herzberg / Ada Yonath /
Abstract: Giardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs is spreading, new strategies for combating ...Giardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs is spreading, new strategies for combating giardiasis need to be developed. The G. lamblia ribosome may provide a promising therapeutic target due to its distinct sequence differences from ribosomes of most eukaryotes and prokaryotes. Here, we report the cryo-electron microscopy structure of the G. lamblia (WB strain) ribosome determined at 2.75 Å resolution. The ribosomal RNA is the shortest known among eukaryotes, and lacks nearly all the eukaryote-specific ribosomal RNA expansion segments. In contrast, the ribosomal proteins are typically eukaryotic with some species-specific insertions/extensions. Most typical inter-subunit bridges are maintained except for one missing contact site. Unique structural features are located mainly at the ribosome's periphery. These may be exploited as target sites for the design of new compounds that inhibit selectively the parasite's ribosomal activity.
History
DepositionOct 6, 2021-
Header (metadata) releaseApr 20, 2022-
Map releaseApr 20, 2022-
UpdateApr 20, 2022-
Current statusApr 20, 2022Processing site: PDBe / Status: Released

-
Structure visualization

Supplemental images

emd_13681.png

Downloads & links

-
Map

FileDownload / File: emd_13681.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 440 pix.
= 374. Å		0.85 Å/pix.
x 440 pix.
= 374. Å		0.85 Å/pix.
x 440 pix.
= 374. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.85 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.034
Minimum - Maximum-0.10845332 - 0.24637817
Average (Standard dev.)0.00028543535 (±0.005771762)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 374.0 Å
α=β=γ: 90.0 °

-
Supplemental data

-
Sample components

+
Entire : Giardia lamblia ribosome

EntireName: Giardia lamblia ribosome
Components
  • Complex: Giardia lamblia ribosome
    • RNA: rRNA 28S
    • RNA: rRNA 5S
    • RNA: rRNA 5.8S
    • Protein or peptide: Ribosomal protein L2
    • Protein or peptide: Ribosomal protein L3
    • Protein or peptide: Ribosomal protein L4
    • Protein or peptide: Ribosomal protein L5
    • Protein or peptide: Ribosomal protein L7
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: Ribosomal protein L6
    • Protein or peptide: Ribosomal protein L10
    • Protein or peptide: Ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: Ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: Ribosomal protein L13a
    • Protein or peptide: Ribosomal protein L17
    • Protein or peptide: Ribosomal protein L18
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: Ribosomal protein L21
    • Protein or peptide: Ribosomal protein eL22
    • Protein or peptide: Ribosomal protein L23
    • Protein or peptide: Ribosomal protein L23A
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: Ribosomal protein L27a
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: Ribosomal protein L30
    • Protein or peptide: Ribosomal protein L31B
    • Protein or peptide: Ribosomal protein L32
    • Protein or peptide: Ribosomal protein L35a
    • Protein or peptide: Ribosomal protein L34
    • Protein or peptide: Ribosomal protein L29
    • Protein or peptide: Ribosomal protein L36-1
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: Ribosomal L38e
    • Protein or peptide: Ribosomal protein L39
    • Protein or peptide: Ubiquitin/Ribosomal protein L40e
    • Protein or peptide: Ribosomal protein L44
    • Protein or peptide: Ribosomal protein L37a
    • RNA: E-site tRNA
    • Protein or peptide: Ribosomal protein L24A
    • RNA: P-site tRNA
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM ION
  • Ligand: TRIETHYLENE GLYCOL
  • Ligand: water

+
Supramolecule #1: Giardia lamblia ribosome

SupramoleculeName: Giardia lamblia ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
Source (natural)Organism: Giardia intestinalis (strain ATCC 50803 / WB clone C6) (eukaryote)

+
Macromolecule #1: rRNA 28S

MacromoleculeName: rRNA 28S / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Giardia lamblia (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 878.2545 KDa
SequenceString: GCGCGGCCCG AGGCGGCGGG GGCGACGGGC GGAACUUAAG CAUAUCAG(OMU)A CGCCCCGGAG GAGAAACCAA CCGGGA UUC CCCGUAGCGG CGAGCGACGC GGGAGGAGCC CGCCCCGAAG GCGCGCUGUG GGGCGCAGGC GCAGGCCCGC CGCGAGG GG GCCCGAGGGC ...String:
GCGCGGCCCG AGGCGGCGGG GGCGACGGGC GGAACUUAAG CAUAUCAG(OMU)A CGCCCCGGAG GAGAAACCAA CCGGGA UUC CCCGUAGCGG CGAGCGACGC GGGAGGAGCC CGCCCCGAAG GCGCGCUGUG GGGCGCAGGC GCAGGCCCGC CGCGAGG GG GCCCGAGGGC CCCGCCCGAG AGGGUGCAAG CCCCGUACGG CGGCCGCCGG GCCUGCGCGG CGAGUAGCGC UGCUUGAG C GUGCAGCGCG AAGGGAGGCG CGGCCCUUCC AAGGCUAAAU ACGCCCCGGG ACCGAUAGCG GACCAAGUAG CGCGA (OMG)CGA ACGGUGAAAA GGACGCCCUG CGGCCGCUCA AAAGACCUGA ACCCGGCCGG CCGCCGGCCC GCCGGCCCC (OMG)UCUCGA(A2M)AC (A2M)CGGACCGAG GAGCCACGCG CCGCGGCGAG CCCGAGGGAG CCCCCGCGGC GGAGCGAG C GCGAGACGCC CCGGGCCCGC CGCGCCCCUG CGGGCGUGCG CGGGCCGAGC CGCGGCGCGU GGGCCCGA(A2M)A GGCGG UGAU CUAUGCCCGG CGAGGGCGAG GCCGGGCGAA AGCCUGGUGG AGGCCCGCCG CGGUGCUGAC GCGCAGAUCG CUCGUC GGA GCCGGGCAUG G(OMG)GGCGAAAG ACUCAUCGAA CCGCCUGGUA GCUGGUUGCC UCCGAAAUGU CUCCCAGGAC AGC CGCCGC CCCGCAGUUG CGGCCCGUAG AGCGCUGGCC GGCGGGAGCG GGGGGCCUGC CCCUCGCCCG CCCCCCAAAC UCCG AAGGG CCGCGCCGCC CCGCCGCUGG CCUGGGCGGG GCGGGCGAAU GCGGGCGGCG CGUGGGCCCC UCCUGGUAAG CAGGA CGGG CGAGGCGGGA CGAUCCGGAC GCCGGGCCAG GGUGCGCCGC CGGGGCCCGC GGAACGGCGU CGGCCGGUCC CGACAG CUG GAAGGUGGCC CCAGAAGUCG GCAUCCUCCA GGGAGUGUGU AACAACCCAC CAGCCGAAUC GGCCGGCCCG GAAAAUG GA GCGCGCCGGA GCCCCGGACC CGCGCCCGGC CGCCGCGCGC GGCGGGUAGG AGGCCGCAGA GGCCCCGGGG GCGAAGGC G GCGCGCAGGC CCCGCCGGAC CGGCCUCUGG UGCA(OMG)AUCUC GGCAGCAGUA GCCGCUACUC CGCGCCCCGG AGGAC UGAG GGGGAGACGG GUUCCGCGGC GCCUGCAUCU GGCCGCGG(OMG)U GACUCGGGCC UAAGCGGCGG GUGAAGACCG GG AAGGGGC GUGCCCGCCC GUCGAACGGG GAGCCGGCGG AGACUCCGGC AGGCGCGGCC CCCGCGGAGA CGCCCGCCCC CCG GCGACG CGCACGGGGA CCGCGGCGGG CGGCGCCCCG GCCCGCGAAC GCCCCGCAGC CCCCGGACGC CUUGCGCGGA GAGG GGGGC CCGGGGGCGG ACCCCGCGCG UCCCCGGCCG CCCCUGAAAA GCCGGGGGGC GCCGGCCGCG CGCCGUACCG ACCGC AGCA GGACUCCGGG GUCAGCAGCC UCUAGCGCGG GAGCGAAC(OMG)C GGCUCAGGGA AGUCGGCAAG CCGGCUCCGU AA CCUCGGG AAAAGGAGUG GCUCUGACGG CGCGCCGGGU CAGAACUGGA ACGGACGCGG GGAUCCCGAC UGUUUACUAG AAA CACAGC GUCGCGAGGG CCGCACCCGG CGCUGGCGCG ACGUGAUUUC UGCC(OMC)AGUGC CACGACCGUC ACCGUGAAGC GAUCCGCCG AAGCCCUGGU AAACGGCGGG AGUAACUAUG ACUCUCUUAA GGUAGC(5MC)AA(A2M) UGCCUC(OMG)UC GGGCAAUUUC CGACGUGCAU GAAUGGACCA ACGAGGAUCC CACUGU(OMC)CCG AGCCGCGCCU CCGCGAGCCU CCAGCC UCG GGAACGGGCG AGGGCCGGCC AGCGG(OMG)GCAA GAAGACCCU(OMU) (OMU)UGAGCUUGA C(OMU)CCAGCCCG G GCCUGUGG GGCGGGGCGG CCGGCGCAGC GCACAGGGGA GGCCGCGCCC CUGAGACACC CUGACGGCCG CCGCCGCCCC GC UCACCCG GUCGCGCGGG GACCCGCCCG GGCGGGGAGU UCGGCUG(OMG)GG CGGCGCGCCU GCUACACCGG ACCGCAGGC (OMG)UCCCACGGC GGGCUCAGCG AGGACGGAGA CCUCCCGCGG AGCAGAAGGG CACAAGCCCG CCCGACCCGC GCCCCC CGU GCCGGCGCGG GCCGCGAAAG CGGGGCCUAC CGAUCCUUCG CCGCCCCGGC CGCGGGCGCG GAGGUGGCAG AAAAGUU AC CACAG(OMG)GAUA ACUGGCUUGU GGCCGCCGAG CGCCCGCAGC GACGCGGCUU UUUGAUCCUU (5MC)GAUGUCGGC UCUUCCUAC CGUCCGCGCG CACCGGCGCG GAAGCGUCGG AUUGUUCACC CGUUCAAGGG AUCGUGAGCU GGGUUUAGA (OMC)CGUCGUGAG ACAGGUUAGU UUUACCCUAC UGGCCCCGGG GCCAGAGCAC GGCGGGCCAG UACGAGAGGA ACGCCC GCC GCGGGCGCCC AGCCCCGCGG UUGCCCGCCG GGGCAGGACC GCGCGCCCGG GCCCGGGGGC CUGGCGCUGC CGCCUCU AA AGCGCCACCC CCCCCUCCGG CCCCGCCGGG CCCGCGCCCC AGCCCCGUGC CCCCUGCCCG AGGCGGCCCC CGCCCGGG A GGACCACCCG GCGCGGCGCC CCUGUACGGC GCAGGGCCUG CGAUCGCGUU CGCCCGGGGG GCGCGCCGGG CGGGCGCGC GGCCCACUUG CU

+
Macromolecule #2: rRNA 5S

MacromoleculeName: rRNA 5S / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Giardia lamblia (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 38.671039 KDa
SequenceString:
CAGUUCGGCC AUCCUACGGC GGAAACUCCU UAACUCGUUC CGAAUUAAGA AGAGAAGCGC CGUCAGGCCC CGCCAGUACU GCGAUCGGA GACGUCGUGG GAACACGGGG UGCCGAACCU U

+
Macromolecule #3: rRNA 5.8S

MacromoleculeName: rRNA 5.8S / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Giardia lamblia (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 44.971965 KDa
SequenceString:
GAACGCCCCG CCGGCGGAUG CCUCGGCCCG GGCGGCGACG AAGAGCGCGG CGGAGCGCGA GACGCGGUGC GGACCCGCCC GCCCCGAGA AGCACCGACC CUCGAACGCA GCGCGCCCCG GCGCCGCCGC CUC(OMG)GCGCCC

+
Macromolecule #42: E-site tRNA

MacromoleculeName: E-site tRNA / type: rna / ID: 42 / Number of copies: 1
Source (natural)Organism: Giardia lamblia (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 24.500553 KDa
SequenceString:
AGCGGGUUAG CUCAGUCGGU AGAGCGUCAG UCUGAUAAGC UGAAGGUCGG GGGUUUUCGA GCCCCCCCCG CCACCA

+
Macromolecule #44: P-site tRNA

MacromoleculeName: P-site tRNA / type: rna / ID: 44 / Number of copies: 1
Source (natural)Organism: Giardia lamblia (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 894.612 Da
SequenceString:
CCA

+
Macromolecule #4: Ribosomal protein L2

MacromoleculeName: Ribosomal protein L2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 27.090543 KDa
SequenceString: MGRRIRVQRK GAGSVFRAHV ARRLGAAKLR ANDFAERTGS VRGVVKAILH DRGRGAPLAR VQFPNMRGEG RVNELFIAPE GMYTGQEVF SGNKATLHIG NILPVGNIPE GTYVCNVEEK PMDRGCLARA SGTYCLVVAH NMETNKTRIR LPSGQKKLIS S KARAMIGL ...String:
MGRRIRVQRK GAGSVFRAHV ARRLGAAKLR ANDFAERTGS VRGVVKAILH DRGRGAPLAR VQFPNMRGEG RVNELFIAPE GMYTGQEVF SGNKATLHIG NILPVGNIPE GTYVCNVEEK PMDRGCLARA SGTYCLVVAH NMETNKTRIR LPSGQKKLIS S KARAMIGL VAGGGRTDKP LLKAGNAYHK YKAKRNCWPV VRGVAMNPVD HPHGGGNHQH IGVSSCVPRN AVPGQKVGLI AA RRTGCSG GKRR

+
Macromolecule #5: Ribosomal protein L3

MacromoleculeName: Ribosomal protein L3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 42.715543 KDa
SequenceString: MSHRKFSCCR KGNLGYLPRK RCTRGRGRCK TFPPDVQGAP IHLTAFLGYK AGCTHVMRFI DHRGSNLHNR QAIDQATIID APPMICTAI IGYAKTPKGL RAVTTVWAAH IAEPAMRRFY RNYFHAEKTA FSTYMKKAAD GTYIKEQLGR LKQHADVIRI V AHTQPALT ...String:
MSHRKFSCCR KGNLGYLPRK RCTRGRGRCK TFPPDVQGAP IHLTAFLGYK AGCTHVMRFI DHRGSNLHNR QAIDQATIID APPMICTAI IGYAKTPKGL RAVTTVWAAH IAEPAMRRFY RNYFHAEKTA FSTYMKKAAD GTYIKEQLGR LKQHADVIRI V AHTQPALT PLKQKKADIM EIQVNGGANV AEKVDYAYAL MEKPISIKDV FSVGAQIDTI SITRGRGFEG VVTRWGVTRL PR KTRRGNR KVACIGAWHP ANVQYTVARA GQMGYFHRTD TNKQVFMIDT AENPRCCTTE FDLTDKSINP VGGFVNYGRV QGD FIMIKG TCPGPKRRPV VMRNALLPHR NCPPVQLQWI STASKFGHGI FETVEERRQF YAK

+
Macromolecule #6: Ribosomal protein L4

MacromoleculeName: Ribosomal protein L4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 35.048707 KDa
SequenceString: MNPTVKVFGP TGTQVAELPR PKVFNVPLRP DIINFVHTQL RKCLRTPYAV SRYAGVQCTA HSWGPGRAVA RLPRKHGGIG AYANFARGG HMAHPTTVIR RWCRKVNLNQ RRYAVASALA ASANAALVEA RGHRIADVPS IPLVVDTDNV TKTKDALAII K AVGVLRDV ...String:
MNPTVKVFGP TGTQVAELPR PKVFNVPLRP DIINFVHTQL RKCLRTPYAV SRYAGVQCTA HSWGPGRAVA RLPRKHGGIG AYANFARGG HMAHPTTVIR RWCRKVNLNQ RRYAVASALA ASANAALVEA RGHRIADVPS IPLVVDTDNV TKTKDALAII K AVGVLRDV ERCKDSRHIR AGRGKMRNRR YITRKGPLVI YNTEDVARGF RNLPGVDLCH VSSIRLLELA PGSHPGRLIL WT KSAFASL DDVYAAKQNY TLPRSCITQT DIERILQSDA VQKTFVPKRD PLVIERKVDP FSSKEALARL DPSGTVMPK

+
Macromolecule #7: Ribosomal protein L5

MacromoleculeName: Ribosomal protein L5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 33.961375 KDa
SequenceString: MGWVKVQKTR SYFKRFQVQF RRRREGKTDY QARKALIIQD KNKYATPRYR FVVRRTNKDI ICQVTAAELT KDRVVCAAYS HELPRYGIP VGLTNYAAAY ATGLLCGRRL LEKLSVGIKE NALAQLYPGL DKATGEEYHP DENENEEVHA FKAFLDIGLA R ASTGARVF ...String:
MGWVKVQKTR SYFKRFQVQF RRRREGKTDY QARKALIIQD KNKYATPRYR FVVRRTNKDI ICQVTAAELT KDRVVCAAYS HELPRYGIP VGLTNYAAAY ATGLLCGRRL LEKLSVGIKE NALAQLYPGL DKATGEEYHP DENENEEVHA FKAFLDIGLA R ASTGARVF AAMKGAVDAG LNIPHSMKRF PGYSKDGFDS SALRDRIMGK HVGDYMAELK AEDEETYRKR FSKYIAAKVE PL RLEAMYT KAHAAIRANP QAVKAKFEIK KPVRQARLTL KERKERIDAK KLEALKQLTK

+
Macromolecule #8: Ribosomal protein L7

MacromoleculeName: Ribosomal protein L7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 27.103406 KDa
SequenceString: MPIPELVLKH RRKVQRDIRS AAERATEKEK RAALVKDHAV KSAQVYARYY IDLVESEKKA RENALKNNQL YVPDEPKVYL VVRIRGIVG VAPKVRTILS LLRLRQINNA VFMRCNKATL NALRIVDHYV TYGEPTVETI RNLIYKRGFA KVNGQRIPIV D NNLIDEQL ...String:
MPIPELVLKH RRKVQRDIRS AAERATEKEK RAALVKDHAV KSAQVYARYY IDLVESEKKA RENALKNNQL YVPDEPKVYL VVRIRGIVG VAPKVRTILS LLRLRQINNA VFMRCNKATL NALRIVDHYV TYGEPTVETI RNLIYKRGFA KVNGQRIPIV D NNLIDEQL SKHNILCAED LVHEIFTCGS SFKEANNFLW PFQLNSAALK DKRNNFAEGG DFGYRGKYIN EFVARMI

+
Macromolecule #9: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 25.427725 KDa
SequenceString: MSKVSGSDIK RALAVPENKS RSKCDFDLTP FVRWPRQVRI QRQKAVLQRR LKVPPTVNQF MNPISRNLTN EIFNLARKYS PESKEEHKA RLLQIADAKA NGKPLPEKSD KLVIASGIRR ITSLVESKRA KLVLIANDVD PLELVLWLPT LCHKMGVPYA I VRTKGDLG ...String:
MSKVSGSDIK RALAVPENKS RSKCDFDLTP FVRWPRQVRI QRQKAVLQRR LKVPPTVNQF MNPISRNLTN EIFNLARKYS PESKEEHKA RLLQIADAKA NGKPLPEKSD KLVIASGIRR ITSLVESKRA KLVLIANDVD PLELVLWLPT LCHKMGVPYA I VRTKGDLG KLVHLKKTTS VCFTDVNPED KPTFDKILAA VAHEVDYAKA MKTYGGGVRR EDEAQQM

+
Macromolecule #10: Ribosomal protein L6

MacromoleculeName: Ribosomal protein L6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 20.889379 KDa
SequenceString:
MPLCSSMTIK VPTDVECTIN KRVVTVKGKK GTLVRDFSHA PLDITHANDT ISVAVWFPRG KRNALPRTIC SHIENMFKGV TYGFEYKMR LAYAHFPIAA TVVDNNKAIE IRNFMHQIKT RRIECLPGVT IAMSTNVKDE LILRGISIED VSHTAARIHE S LKVPNKDL RKFLDGCYVS SRGLQEQ

+
Macromolecule #11: Ribosomal protein L10

MacromoleculeName: Ribosomal protein L10 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 24.283172 KDa
SequenceString: MGRRPARCYR YQKNKPYPKS RFCRGVPDPK IRYFDLGNRR AKVNDFPYCF HLLSGEKEQV SSEALEACRI ACNKYIAKKA GKDSFHLRI RVHPFHVIRI NKMLSCAGAD RLQTGMRGAW GKPQGTVARV SIGQPLLSVR CRASAKDYVK DALRRAKFKI P GRQAIVES ...String:
MGRRPARCYR YQKNKPYPKS RFCRGVPDPK IRYFDLGNRR AKVNDFPYCF HLLSGEKEQV SSEALEACRI ACNKYIAKKA GKDSFHLRI RVHPFHVIRI NKMLSCAGAD RLQTGMRGAW GKPQGTVARV SIGQPLLSVR CRASAKDYVK DALRRAKFKI P GRQAIVES RNWGFTEFTK EEYEDLRERG ELQYDGNNAH RISRKGPLNL KL

+
Macromolecule #12: Ribosomal protein L11

MacromoleculeName: Ribosomal protein L11 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 19.767684 KDa
SequenceString:
MSQESENPMR RIRIEKLVLN ICIGGEGDAL TKAAKVLQQL TDQEPTFSKS RLTIRGFGIR RGQKISTHVT VRGEKAREII ERGLRVKNF ELPYSCFSTT GNFGFGITEH IDLGIKYDPS MGIYGMDFYV VLSRPGFRVN KRRAQQQHVG ASHKITPDEA R AWVEREFE VVYTK

+
Macromolecule #13: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 26.640346 KDa
SequenceString: MASYASFILR LIHFNLNFVT AFCPLPRAMG RNVAFSNMRR YKCTNKGPNL IKNWFNQPAR KIRRRQTRLQ KAKAIFPCPL QKLRPSVTR CSQKFNLTQR IGRGFTLREL QGVGLSAGKA RQIGIAVDPR RKNKSEESVA RNVARLKEYL SKVTIFSKQA K PEEIQQAI ...String:
MASYASFILR LIHFNLNFVT AFCPLPRAMG RNVAFSNMRR YKCTNKGPNL IKNWFNQPAR KIRRRQTRLQ KAKAIFPCPL QKLRPSVTR CSQKFNLTQR IGRGFTLREL QGVGLSAGKA RQIGIAVDPR RKNKSEESVA RNVARLKEYL SKVTIFSKQA K PEEIQQAI QHQGVVMPVV NPKPVITTGS IAEAKKEVPE NLFMYLRNAR TARNRARLAK RDAEREAAAK TKGGKK

+
Macromolecule #14: Ribosomal protein L14

MacromoleculeName: Ribosomal protein L14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.785472 KDa
SequenceString:
MSYHRFVEAG RLVYLGDGPD KGKLAVIVDI LSTAAVLIDG PTTGVKRQVV SLRWAMLTDQ VINISEKPTT EQLKKEIEAS PAIDTFMNS VWGKKIQQQR LRAKATFEDM GKIAKLRRER KMAMKRIAMS IK

+
Macromolecule #15: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 24.35373 KDa
SequenceString: MGAYKYVQEL WRKKQSEALH YLFRLRTWEY RQLNAIKRCS HPTRPEKARR LGYKAKRGYV VYRVRVRRGG RKRQCVHGIS YGKPRNIGI VGLKPSRSLR ALAEQKVGRR LPSLRVLNSY WVAQDGSYKW YEVILIDPFH PWIRNDPKIN WICNPVMKRR E CRGLTSAG ...String:
MGAYKYVQEL WRKKQSEALH YLFRLRTWEY RQLNAIKRCS HPTRPEKARR LGYKAKRGYV VYRVRVRRGG RKRQCVHGIS YGKPRNIGI VGLKPSRSLR ALAEQKVGRR LPSLRVLNSY WVAQDGSYKW YEVILIDPFH PWIRNDPKIN WICNPVMKRR E CRGLTSAG RKARGLHNKG IGAMKLRPSK RAAWKRNHTR SLRRYR

+
Macromolecule #16: Ribosomal protein L13a

MacromoleculeName: Ribosomal protein L13a / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 22.921914 KDa
SequenceString: MSRIVIDCKG HILGRLSAIV AKELLHGHEV ALVRCEKAII SGAFRDVKAH YLKKMHKRTN FNHLRGPFHF RAPAEIVRRT IRGMIPYKT VRGSDAMSRL RVYDGIPPCC ARYKRVVCPS AMAYLCLRPE RRRCEVGRVA HEIGWQHAAD VERYEKRRLE E CDKWYQDK ...String:
MSRIVIDCKG HILGRLSAIV AKELLHGHEV ALVRCEKAII SGAFRDVKAH YLKKMHKRTN FNHLRGPFHF RAPAEIVRRT IRGMIPYKT VRGSDAMSRL RVYDGIPPCC ARYKRVVCPS AMAYLCLRPE RRRCEVGRVA HEIGWQHAAD VERYEKRRLE E CDKWYQDK IQIDKKIAEA AEKDAELQRI NAELAKYGY

+
Macromolecule #17: Ribosomal protein L17

MacromoleculeName: Ribosomal protein L17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 18.722785 KDa
SequenceString:
MVKYAFKSDN LLVGKSRRDG VKASFKNTYS TCRAIKGMGV KRAFAYLDNV LNHKEIVPFF KFYKGVGRHS QARGSGTDKG RWPEKSVVV VKDILKQAVS NAKHAHKKEE DELYVISYCV NRARGRRRRT FRAHGRISKY ESQPCHVELI VGPKPLDVEK A KALQ

+
Macromolecule #18: Ribosomal protein L18

MacromoleculeName: Ribosomal protein L18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 20.207768 KDa
SequenceString:
MGIDLKTGIK KRKVERKEPK SPNPYIRLLH NAFKVVYENT GEDFTKVLTK RLCLSKTNRP VLSVSRLAHL MHKREDKIAV VASTVTNDE RLVTIPRMTI CALKFSATAR SRIEKAGGRC MSFDELLAEK PTGDNCVLLQ GRRSCRAVCK KFGSPGRPNS H VKPKGEHK GRRGARGRHS W

+
Macromolecule #19: Ribosomal protein L19

MacromoleculeName: Ribosomal protein L19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 23.199238 KDa
SequenceString: MANLRLQKRL AADVLKCGKR RVRFNPAHEK YIEQASSREA IRSLIESKII AKLDIKGSSR GRIRARHAAK RLGRHSGHGK RRGTREARM PSKTLWIQRQ RVLRRLLSRY RDDEKIDRHL YETLYAKAKG NMFKNKRVLL EHIVGMQKEQ ELEKKKKEEL E QRRKKAEQ ...String:
MANLRLQKRL AADVLKCGKR RVRFNPAHEK YIEQASSREA IRSLIESKII AKLDIKGSSR GRIRARHAAK RLGRHSGHGK RRGTREARM PSKTLWIQRQ RVLRRLLSRY RDDEKIDRHL YETLYAKAKG NMFKNKRVLL EHIVGMQKEQ ELEKKKKEEL E QRRKKAEQ RCKERAEKVA ANRLAFAQTG IFTEKTNK

+
Macromolecule #20: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 20.624902 KDa
SequenceString:
MMQKYIVYGR RLPSEKFPEP EVFRMTIYAP NEIVAKSRFW YHLKSLKKIK RTHGEILDCQ HIEENGDFVK NFGVLLRYRS RVGQHNMYR EVRETTSARA MDKVYSEMAG QCRARYHDIQ IINVKEVADE DVRREKVAMF TQKGVRFPHP CPYVHVKRAA R TARFQDRA PHVPQ

+
Macromolecule #21: Ribosomal protein L21

MacromoleculeName: Ribosomal protein L21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 18.296393 KDa
SequenceString:
MTHSQGYRCK TRYCFARKFR RHGMANLTTY NRVYKLGQYV NIKVNGAYHK GMPHRYYNGR TGTIFNIGPR ALGVQIQKRV RGRIMLKRI NVRLEHVHPS DCRKNHLERV AANDAARRLA KETGQPVPCL KRQNALPRAA HVVSIASAID ITPLKYDGVF

+
Macromolecule #22: Ribosomal protein eL22

MacromoleculeName: Ribosomal protein eL22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 19.942402 KDa
SequenceString:
MEGQWILFPD QIVNHLCRHA DHRLQARVSS KSNLKSNYAY YFSPSSEMRP RIKKCVKKEQ GNIRFAVDCS AGKDLELNLN EFADYVRTH FKINNRKHGV AEKVACSVEG DSLVIQTTGY EFAKRYVKYL TKRFLYQDYQ GVFRVLSTDK ETYTLKPYTI E DDDDEAGT DDE

+
Macromolecule #23: Ribosomal protein L23

MacromoleculeName: Ribosomal protein L23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.45004 KDa
SequenceString:
MASKRGRSGG IGNKFRISCG LPVGAVINCA DNSGAKSLNM IAVKNWKGHL NRFPKASVGD MVLITVGRDG KPELRRKVTP AVVVRQRKV WRRKDGVWIY FQDNAGVIIN PKGELKGSSI TGPVAKEAAD RWPKISTTAE CVH

+
Macromolecule #24: Ribosomal protein L23A

MacromoleculeName: Ribosomal protein L23A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 16.053919 KDa
SequenceString:
MSEKAQALKA QKAILHGTRV EAKKIRTNVH FFRPKTLVHK KAPLYPRNPI KKGSWDDPCD LLRYPIKTDE NTTQIEKNNT IVFIVDRRA SKPEIRKAFE TVFETKVARV NTLITPLGLK KAFIRLAPDV QAMDVASKMG LA

+
Macromolecule #25: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.621398 KDa
SequenceString:
MKLNSAVTAS RRKCRKAYFT ANAETRAKMM SSRLSKELRA EHKIKTMPIR RGDIVEIFTG GHKGTGKVVE VRRRDYKICV EGINQKARN PEAKPVPYPI HPSNCIIKEL YMNGSRYRAI KRRQERNAER LARIGK

+
Macromolecule #26: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.374895 KDa
SequenceString:
MSQPIIKPGL VCIVLQGRHA GKKAVIVEAL PHNERGSYAI VAGIAALPRR ITNSMSDEEA VCRGRVRSFV KCMNVNHLMP TSCRLENNY KDFISTQTVA DVTKRREIIE TVNKDFQRRI LEHRDTWFFT KLPIDA

+
Macromolecule #27: Ribosomal protein L27a

MacromoleculeName: Ribosomal protein L27a / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 17.166957 KDa
SequenceString:
MPTRIRKSRK RRGHRQMGYG RVGGHRKHPS GRGKAGGLQH KRTSFDKYHP GYFGKLGMRN YHVRRNIEYN PTINIDKIWS LIPEEQRDG FLQRKSTTEA PVVDVTRFGF FKVLGRGSLP QCPIIIKARY FSSDAEKKLR EVGGVPLLVP

+
Macromolecule #28: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 7.363574 KDa
SequenceString:
MAKLKNHTSK NQNRKDHRNG IKKPKKSAYT SHKGMCPKYL RNLRRSRAND PRQSLRPNLN KE

+
Macromolecule #29: Ribosomal protein L30

MacromoleculeName: Ribosomal protein L30 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 11.698598 KDa
SequenceString:
MSTKVATKKA QDSISLTLTL VVKSGKYTLG TDQAIRNIRD GKAQLVFMAN NCPPLVKSQV DYLCHLSKTP VRVFAGSSRE LGIAVGRQF NVSIMTILEA GDADLTSILG

+
Macromolecule #30: Ribosomal protein L31B

MacromoleculeName: Ribosomal protein L31B / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 12.184064 KDa
SequenceString:
MGIVYEHTIR LNKAVRGKPS KKAAPIAIRA IRAQVEKLAK VEDVRLDPSV NVFVWSRGIR HVPRRIRLEV RLEGDDEHKF AYVINKDVE NFKHLTTEKK GSEDGSE

+
Macromolecule #31: Ribosomal protein L32

MacromoleculeName: Ribosomal protein L32 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 15.985983 KDa
SequenceString:
MAGEMIHPLP HKIIIKKRKN KFIRFQSERF VRVKPSWRKP RGIDSPMRHQ YRGQRPMVSI GYGSDKETRF MLPCGFFKHR ISTVHELKT LFMNNKTHAA QIAHGVSARS RKQILEEAKK MGVRVLNADA KLRKEEL

+
Macromolecule #32: Ribosomal protein L35a

MacromoleculeName: Ribosomal protein L35a / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 13.913122 KDa
SequenceString:
MEEIKGKTFL PAIFVGYQRS RAKQRENVAL VKVSGIYTRA ETDKVLGRLI AIIHSKSGKP IFGKICRPHG NSGMVRARFY TNLPGQLMG HKVRVFYDNI DVGDRATPKY ERLYGNPNAT VTYE

+
Macromolecule #33: Ribosomal protein L34

MacromoleculeName: Ribosomal protein L34 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 13.528753 KDa
SequenceString:
MADCRVTCRF HSTYRTRSKI VRYKRTPGNR LTSLRVKKLP NAPHCAETGK QIHGIPRVIP QHLTRQQRKV SRPYGGKLCG SALRAHILE SFLNEENQAL REIAAQTAKT AKQTAGKGHA K

+
Macromolecule #34: Ribosomal protein L29

MacromoleculeName: Ribosomal protein L29 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.292209 KDa
SequenceString:
MPGLKAKDLV GLSQEDLQRK LADLKRELLS LRTMKATAGP VPERIARFRV CKKDVARVLT VINQKARDEA RAAFEGAEHI PKTFRPRLT HAMRCQLTEK QKRLLPSKLM KRKLQFPKLK YAVKV

+
Macromolecule #35: Ribosomal protein L36-1

MacromoleculeName: Ribosomal protein L36-1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 10.586708 KDa
SequenceString:
MPGKVFNLKK GGAVVRIVRK KEERKTKPHQ EFVKSIIQEC TGMAPYEMHI IELLRMNKDR HALRYAKKRL GNIKRAKAKR DQLSVYARN I

+
Macromolecule #36: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 10.329022 KDa
SequenceString:
MSKGTASFGK RHTRIHGSCR RCGKHSFHLQ KHECASCGYP SAKMRRYNWS YKSLRRRTQG TGSMSHMRKV YRAYNSGTLK ANARKYLAK

+
Macromolecule #37: Ribosomal L38e

MacromoleculeName: Ribosomal L38e / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 8.605145 KDa
SequenceString:
MPNTITEENL MKMIADGRGK KLVVKSCSKG RLQGKVKLKL RTAKYLYTLT VPNADDAKSI VKGCKDKVEV QYVNDSE

+
Macromolecule #38: Ribosomal protein L39

MacromoleculeName: Ribosomal protein L39 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 6.353656 KDa
SequenceString:
MTANKTLSRK LRLAKKTKTN KNIPRWVIAK DHLKKTWNYK RHHWRRSHLK L

+
Macromolecule #39: Ubiquitin/Ribosomal protein L40e

MacromoleculeName: Ubiquitin/Ribosomal protein L40e / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 14.343675 KDa
SequenceString:
MQLIVRSLDR TVALTASPAD SLTSIRQRLL AVYSGHVVDS QRFVFAGRTL DEAKTLGDYS IGESSVLDLV PRLFGGVMEP TLINLAREY NQNMMICRHC YARNPLRATN CRRCRGTNLR KKKLSKYA

+
Macromolecule #40: Ribosomal protein L44

MacromoleculeName: Ribosomal protein L44 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 12.311678 KDa
SequenceString:
MVTYPAERRT FCKREGKHTV HKASLYKKGK DSLLAQGKRR YDRKQRGFGG QTKPIFRRKA KTTKKQVIRL TCSSCKCQTF QVLKRCHRL EISRLGATKK SKGSVVF

+
Macromolecule #41: Ribosomal protein L37a

MacromoleculeName: Ribosomal protein L37a / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 10.508366 KDa
SequenceString:
MAKRTKKVGL TGKYGTRYGR AARKQLLKIE TSQKAKYTCP FCAKDKVRRT ACGIWTCRSC SKTMAGGAYS LHTEPANAAR ATIRRLRLA REDAQ

+
Macromolecule #43: Ribosomal protein L24A

MacromoleculeName: Ribosomal protein L24A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia lamblia ATCC 50803 (eukaryote) / Strain: ATCC 50803 / WB clone C6
Molecular weightTheoretical: 12.005191 KDa
SequenceString:
MVTTNQCSYS GRKILPGYGK RMSRHDKVLL IFLNRKASVH YLNKWNPRKI RWTLASRRAR GKEVIVKARE RVTRKAVVSK RNFLSIDDS KLEALKSKYS RVN

+
Macromolecule #45: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 112 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #46: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 46 / Number of copies: 181 / Formula: K
Molecular weightTheoretical: 39.098 Da

+
Macromolecule #47: TRIETHYLENE GLYCOL

MacromoleculeName: TRIETHYLENE GLYCOL / type: ligand / ID: 47 / Number of copies: 1 / Formula: PGE
Molecular weightTheoretical: 150.173 Da
Chemical component information

ChemComp-PGE:
TRIETHYLENE GLYCOL

+
Macromolecule #48: water

MacromoleculeName: water / type: ligand / ID: 48 / Number of copies: 4397 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.75 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 91058
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more