7PWD

Structure of an inhibited GRK2-G-beta and G-gamma complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-adrenergic receptor kinase 1	polymer	697	80649.7	1	UniProt (P25098) Pfam (PF00615) Pfam (PF00069) Pfam (PF00169)	Homo sapiens (Human)	Beta-ARK-1,G-protein coupled receptor kinase 2
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF25391)	Bos taurus (Bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	77	8674.0	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
4	D (A)	4-chloranyl-N-[2-(4-chlorophenyl)ethyl]thieno[2,3-c]pyridine-2-carboxamide	non-polymer		351.3	1	Chemie (8DS)
5	E, G (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	F (A)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
7	H (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8	I, J, K (A, B, G)	water	water		18.0	169	Chemie (HOH)

Sequence modifications

A: 1 - 689 (UniProt: P25098)

PDB	External Database	Details
Ala 670	Ser 670	engineered mutation
Gly 690	-	expression tag
Ser 691	-	expression tag
His 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	126740.6
Non-Polymers*	Number of molecules	5
	Total formula weight	752.5
All*	Total formula weight	127493.1

*Water molecules are not included.