7PTJ
C54S mutant of choline-sulfatase from E. meliloti CECT4857 bound to HEPES
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Choline sulfatase | polymer | 520 | 59495.6 | 4 | UniProt (A0A410NSD4) Pfam (PF00884) Pfam (PF01663) Pfam (PF16347) Pfam (PF12411) UniProt (by SIFTS) (O69787) | Rhizobium meliloti (Ensifer meliloti Sinorhizobium meliloti) | |
| 2 | E, F, NA, P, Z (A, D, B, C) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 5 | Chemie (EPE) | |||
| 3 | AA, BA, CA, DA, EA... (C, A, B, D) | SULFATE ION | non-polymer | 96.1 | 13 | Chemie (SO4) | |||
| 4 | FA, GA, HA, IA, JA... (C, A, B) | GLYCEROL | non-polymer | 92.1 | 17 | Chemie (GOL) | |||
| 5 | MA, N, QA, Y (C, A, D, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 6 | RA, SA, TA, UA (A, B, C, D) | water | water | 18.0 | 1827 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 512 (UniProt: A0A410NSD4)
| PDB | External Database | Details |
|---|---|---|
| Ser 54 | Cys 54 | engineered mutation |
| Gly 513 | - | expression tag |
| Ser 514 | - | expression tag |
| His 515 | - | expression tag |
| His 516 | - | expression tag |
| His 517 | - | expression tag |
| His 518 | - | expression tag |
| His 519 | - | expression tag |
| His 520 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 237982.2 | |
| Non-Polymers* | Number of molecules | 39 |
| Total formula weight | 4166.3 | |
| All* | Total formula weight | 242148.5 |
