7PQO

Catalytic fragment of MASP-1 in complex with P1 site mutant ecotin

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D (C, A, B)	Mannan-binding lectin serine protease 1	polymer	406	45519.0	3	UniProt (P48740) Pfam (PF00084) Pfam (PF00089)	Homo sapiens (Human)	Complement factor MASP-3,Complement-activating component of Ra-reactive factor,Mannose-binding lectin-associated serine protease 1,MASP-1,Mannose-binding protein-associated serine protease,Ra-reactive factor serine protease p100,RaRF,Serine protease 5
2	B, E, F (K, I, J)	Ecotin	polymer	162	18240.0	3	UniProt (P23827) Pfam (PF03974)	Escherichia coli (strain K12)
3	G, I, J, K, L... (C, K, A, I)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
4	H (C)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)

Sequence modifications

C, A, B: 298 - 699 (UniProt: P48740)

PDB	External Database	Details
Ala 294	-	expression tag
Ser 295	-	expression tag
Met 296	-	expression tag
Thr 297	-	expression tag
Lys 499	Glu 499	variant

K, I, J: -19 - 142 (UniProt: P23827)

PDB	External Database	Details
Arg 84	Met 104	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	191277.2
Non-Polymers*	Number of molecules	8
	Total formula weight	667.6
All*	Total formula weight	191944.8

*Water molecules are not included.