7PP4
Cryo-EM structure of Mycobacterium tuberculosis RNA polymerase holoenzyme comprising sigma factor SigB
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | a, b | DNA-directed RNA polymerase subunit alpha | polymer | 347 | 37745.3 | 2 | UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | c | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 129602.3 | 1 | UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | d | DNA-directed RNA polymerase subunit beta' | polymer | 1322 | 147797.8 | 1 | UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | e | DNA-directed RNA polymerase subunit omega | polymer | 110 | 11851.1 | 1 | UniProt (P9WGY5) Pfam (PF01192) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | f | RNA polymerase sigma factor SigB | polymer | 343 | 38572.8 | 1 | UniProt (P9WGI5) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | |
| 6 | d | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 7 | d | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
c: 6 - 1178 (UniProt: P9WGY9)
d: 1 - 1316 (UniProt: P9WGY7)
f: 1 - 323 (UniProt: P9WGI5)
| PDB | External Database | Details |
|---|---|---|
| Met 5 | - | initiating methionine |
| Val 6 | Ile 6 | conflict |
| PDB | External Database | Details |
|---|---|---|
| His 1317 | - | expression tag |
| His 1318 | - | expression tag |
| His 1319 | - | expression tag |
| His 1320 | - | expression tag |
| His 1321 | - | expression tag |
| His 1322 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 403314.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 403469.8 |
