7PMK

S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	DNA replication licensing factor MCM2	polymer	868	98911.5	1	UniProt (A0A6A5Q1S9) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) UniProt (by SIFTS) (P29469)	Saccharomyces cerevisiae (Baker's yeast)	Minichromosome maintenance protein 2
2	B (3)	DNA replication licensing factor MCM3	polymer	1009	111987.6	1	UniProt (P24279) Pfam (PF22207) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (Baker's yeast)	Minichromosome maintenance protein 3
3	C (4)	DNA replication licensing factor MCM4	polymer	933	105138.4	1	UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Saccharomyces cerevisiae (Baker's yeast)	Cell division control protein 54
4	D (5)	DNA helicase	polymer	775	86505.7	1	UniProt (A0A6A5PUY8) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) UniProt (by SIFTS) (P29496)	Saccharomyces cerevisiae (Baker's yeast)
5	E (6)	DNA replication licensing factor MCM6	polymer	1017	113110.2	1	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Saccharomyces cerevisiae (Baker's yeast)	Minichromosome maintenance protein 6
6	F (7)	DNA replication licensing factor MCM7	polymer	845	95049.9	1	UniProt (A0A6A5Q4N0) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) UniProt (by SIFTS) (P38132)	Saccharomyces cerevisiae (Baker's yeast)
7	G (A)	DNA replication complex GINS protein PSF1	polymer	208	24230.6	1	UniProt (A0A6A5Q203) Pfam (PF05916) UniProt (by SIFTS) (Q12488)	Saccharomyces cerevisiae (Baker's yeast)
8	H (B)	DNA replication complex GINS protein PSF2	polymer	213	25096.8	1	UniProt (A0A6A5PX40) Pfam (PF05916) UniProt (by SIFTS) (P40359)	Saccharomyces cerevisiae (Baker's yeast)
9	I (C)	DNA replication complex GINS protein PSF3	polymer	194	22004.2	1	UniProt (A0A6L1BWJ4) Pfam (PF22466) Pfam (PF05916) UniProt (by SIFTS) (Q12146)	Saccharomyces cerevisiae (Baker's yeast)
10	J (D)	DNA replication complex GINS protein SLD5	polymer	294	33983.6	1	UniProt (Q03406) Pfam (PF05916) Pfam (PF16922)	Saccharomyces cerevisiae (Baker's yeast)
11	K (E)	Cell division control protein 45,Cell division control protein 45	polymer	657	75154.7	1	UniProt (Q08032) Pfam (PF02724)	Saccharomyces cerevisiae (Baker's yeast)
12	L, M, N (F, G, H)	DNA polymerase alpha-binding protein	polymer	962	108610.1	3	UniProt (A0A6A5Q5Y5) Pfam (PF12341) Pfam (PF20946) UniProt (by SIFTS) (Q01454)	Saccharomyces cerevisiae (Baker's yeast)
13	O (I)	Leading strand template DNA	polymer	115	37688.6	1		Synthetic construct
14	P (J)	Lagging strand template DNA	polymer	122	35259.2	1		Synthetic construct
15	Q (K)	E3 ubiquitin ligase complex SCF subunit	polymer	194	22357.3	1	UniProt (A0A6A5Q435) Pfam (PF03931) Pfam (PF01466) UniProt (by SIFTS) (P52286)	Saccharomyces cerevisiae (Baker's yeast)
16	R (L)	Protein DIA2	polymer	735	85368.8	1	UniProt (Q08496) Pfam (PF00646)	Saccharomyces cerevisiae (Baker's yeast)	Digs into agar protein 2
17	S (Q)	DNA polymerase epsilon catalytic subunit A	polymer	2222	255890.4	1	UniProt (P21951) Pfam (PF03104) Pfam (PF22634) Pfam (PF08490) Pfam (PF22912)	Saccharomyces cerevisiae (Baker's yeast)	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
18	T (R)	DNA polymerase epsilon subunit B	polymer	689	78425.9	1	UniProt (P24482) Pfam (PF04042)	Saccharomyces cerevisiae (Baker's yeast)	DNA polymerase II subunit 2
19	U (X)	Topoisomerase 1-associated factor 1	polymer	1238	141296.9	1	UniProt (P53840) Pfam (PF04821)	Saccharomyces cerevisiae (Baker's yeast)
20	V (Y)	Chromosome segregation in meiosis protein 3	polymer	319	36588.8	1	UniProt (Q04659) Pfam (PF07962)	Saccharomyces cerevisiae (Baker's yeast)
21	CA, W, Z (5, 2, 3)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	3	Chemie (ANP)
22	AA, DA, X (3, 5, 2)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
23	BA, EA, FA, GA, HA... (4, 5, 6, 7, Q...)	ZINC ION	non-polymer		65.4	7	Chemie (ZN)

Sequence modifications

3: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -37	-	initiating methionine
Lys -36	-	expression tag
Arg -35	-	expression tag
Arg -34	-	expression tag
Trp -33	-	expression tag
Lys -32	-	expression tag
Lys -31	-	expression tag
Asn -30	-	expression tag
Phe -29	-	expression tag
Ile -28	-	expression tag
Ala -27	-	expression tag
Val -26	-	expression tag
Ser -25	-	expression tag
Ala -24	-	expression tag
Ala -23	-	expression tag
Asn -22	-	expression tag
Arg -21	-	expression tag
Phe -20	-	expression tag
Lys -19	-	expression tag
Lys -18	-	expression tag
Ile -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Ala -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Glu -3	-	expression tag
Ala -2	-	expression tag
Pro -1	-	expression tag
Val 0	-	expression tag

E: 1 - 167 (UniProt: Q08032)
E: 167 - 650 (UniProt: Q08032)

PDB	External Database	Details
Asp 167	-	expression tag
Tyr 167	-	expression tag
Lys 167	-	expression tag
Asp 167	-	expression tag
Asp 167	-	expression tag
Asp 167	-	expression tag
Gly 167	-	expression tag
Asp 167	-	expression tag
Tyr 167	-	expression tag
Lys 167	-	expression tag
Asp 167	-	expression tag
Asp 167	-	expression tag

F, G, H: 1 - 927 (UniProt: A0A6A5Q5Y5)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

L: 1 - 732 (UniProt: Q08496)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Gly 0	-	expression tag

Q: 1 - 2222 (UniProt: P21951)

PDB	External Database	Details
Ala 290	Asp 290	variant
Ala 292	Glu 292	variant

Y: 1 - 317 (UniProt: Q04659)

PDB	External Database	Details
Gly -1	-	expression tag
Glu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	22
	Total formula weight	1809879.5
Non-Polymers*	Number of molecules	13
	Total formula weight	2049.4
All*	Total formula weight	1811928.9

*Water molecules are not included.