7PIU
Cryo-EM structure of the agonist setmelanotide bound to the active melanocortin-4 receptor (MC4R) in complex with the heterotrimeric Gs protein at 2.6 A resolution.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Melanocortin receptor 4 | polymer | 346 | 38698.5 | 1 | UniProt (P32245) Pfam (PF00001) | Homo sapiens (Human) | MC4-R |
| 2 | B (P) | Setmelanotide (other names RM-493; BIM-22493; IRC-022493; Imcivree) | polymer | 8 | 1081.3 | 1 | synthetic construct | ||
| 3 | C (A) | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 380 | 44326.2 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Rat) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 6 | F (N) | Camelid antibody fragment - nanobody 35 | polymer | 134 | 14714.3 | 1 | Lama glama | ||
| 7 | G (R) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 8 | H, I, J, K, L... (R, P, A, B, G...) | water | water | 18.0 | 88 | Chemie (HOH) |
Sequence modifications
R: 1 - 332 (UniProt: P32245)
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Lys 0 | - | expression tag |
| Leu 333 | - | expression tag |
| Glu 334 | - | expression tag |
| Val 335 | - | expression tag |
| Leu 336 | - | expression tag |
| Phe 337 | - | expression tag |
| Gln 338 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 144409.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 144449.7 |
