7PHD

Chimeric carminomycin-4-O-methyltransferase (DnrK) with a region from 10-decarboxylase TamK

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein	polymer	369	40144.6	2	UniProt (Q06528) UniProt (I2N5E8) Pfam (PF00891)	Streptomyces peucetius	COMT,Anthracycline 4-O-methyltransferase,O-methyltransferase,COMT,Anthracycline 4-O-methyltransferase
2	C, D, E, I (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	F, J, K (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	G, L (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
5	H, M (A, B)	methyl (1R,2R,4S)-2-ethyl-2,5,7-trihydroxy-6,11-dioxo-4-{[2,3,6-trideoxy-3-(dimethylamino)-alpha-L-lyxo-hexopyranosyl]oxy}-1,2,3,4,6,11-hexahydrotetracene-1-carboxylate	non-polymer		569.6	2	Chemie (3VL)
6	N, O (A, B)	water	water		18.0	334	Chemie (HOH)

Sequence modifications

A, B: 2 - 285 (UniProt: Q06528)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag

A, B: 286 - 302 (UniProt: I2N5E8)
A, B: 303 - 359 (UniProt: Q06528)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80289.2
Non-Polymers*	Number of molecules	11
	Total formula weight	2596.6
All*	Total formula weight	82885.8

*Water molecules are not included.