7PFU

Nucleosome stack of the 4x207 nucleosome array containing H1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, J, N (A, E, K, O)	Histone H3.2	polymer	136	15389.0	4	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/m,Histone H3/o
2	B, F, K, O (B, F, L, P)	Histone H4	polymer	103	11394.4	4	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, G, L, P (C, G, M, Q)	Histone H2A type 1-B/E	polymer	147	16344.9	4	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H, M, Q (D, H, N, R)	Histone H2B type 1-K	polymer	126	13921.2	4	UniProt (O60814) Pfam (PF00125)	Homo sapiens (Human)	H2B K,HIRA-interacting protein 1
5	I, T (U, S)	Histone H1.4	polymer	218	21800.3	2	UniProt (P10412) Pfam (PF00538)	Homo sapiens (Human)	Histone H1b,Histone H1s-4
6	R (I)	DNA (591-MER)	polymer	828	254712.6	1		synthetic construct
7	S (J)	DNA (591-MER)	polymer	828	256865.7	1		synthetic construct

Sequence modifications

A, E, K, O: 0 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

C, G, M, Q: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Ser -5	-	expression tag
Asn -4	-	expression tag
Ala -3	-	expression tag
Pro -2	-	expression tag
Trp -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	783377.1
All*	Total formula weight	783377.1

*Water molecules are not included.