7PFF

Nucleosome 3 of the 4x197 nucleosome array containing H1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (K, O)	Histone H3.2	polymer	136	15389.0	2	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/m,Histone H3/o
2	B, F (L, P)	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, G (M, Q)	Histone H2A type 1-B/E	polymer	147	16344.9	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (N, R)	Histone H2B type 1-K	polymer	126	13921.2	2	UniProt (O60814) Pfam (PF00125)	Homo sapiens (Human)	H2B K,HIRA-interacting protein 1
5	I (I)	DNA (167-MER)	polymer	167	51333.7	1		synthetic construct
6	J (J)	DNA (167-MER)	polymer	167	51774.0	1		synthetic construct

Sequence modifications

K, O: 0 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

M, Q: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Ser -5	-	expression tag
Asn -4	-	expression tag
Ala -3	-	expression tag
Pro -2	-	expression tag
Trp -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	217206.8
All*	Total formula weight	217206.8

*Water molecules are not included.