7PEA

cryo-EM structure of DEPTOR bound to human mTOR complex 1, overall refinement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (B, A)	Serine/threonine-protein kinase mTOR	polymer	2549	287484.2	2	UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260)	Homo sapiens (Human)	FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1
2	B, F (D, C)	Target of rapamycin complex subunit LST8	polymer	326	35910.1	2	UniProt (Q9BVC4) Pfam (PF00400)	Homo sapiens (Human)	TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8
3	C, G (F, E)	Regulatory-associated protein of mTOR	polymer	1396	155963.1	2	UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400)	Homo sapiens (Human)	Raptor,p150 target of rapamycin (TOR)-scaffold protein
4	D, H (J, I)	DEP domain-containing mTOR-interacting protein	polymer	409	46365.8	2	UniProt (Q8TB45) Pfam (PF00610)	Homo sapiens (Human)	DEP domain-containing protein 6
5	I, J (B, A)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	2	Chemie (IHP)

Sequence modifications

B, A: 1 - 16 (UniProt: P42345)
B, A: 17 - 30 (PDB: 7PEA)
B, A: 31 - 36 (UniProt: P42345)
B, A: 37 - 53 (PDB: 7PEA)
B, A: 54 - 355 (UniProt: P42345)
B, A: 356 - 380 (PDB: 7PEA)
B, A: 381 - 2549 (UniProt: P42345)
F, E: 1 - 1335 (UniProt: Q8N122)

PDB	External Database	Details
Met -60	-	initiating methionine
Ala -59	-	expression tag
His -58	-	expression tag
His -57	-	expression tag
His -56	-	expression tag
His -55	-	expression tag
His -54	-	expression tag
His -53	-	expression tag
His -52	-	expression tag
His -51	-	expression tag
His -50	-	expression tag
His -49	-	expression tag
Gly -48	-	expression tag
Ser -47	-	expression tag
Thr -46	-	expression tag
Ser -45	-	expression tag
Gly -44	-	expression tag
Ser -43	-	expression tag
Gly -42	-	expression tag
Glu -41	-	expression tag
Gln -40	-	expression tag
Lys -39	-	expression tag
Leu -38	-	expression tag
Ile -37	-	expression tag
Ser -36	-	expression tag
Glu -35	-	expression tag
Glu -34	-	expression tag
Asp -33	-	expression tag
Leu -32	-	expression tag
Gly -31	-	expression tag
Ser -30	-	expression tag
Thr -29	-	expression tag
Ser -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Asp -24	-	expression tag
Tyr -23	-	expression tag
Lys -22	-	expression tag
Asp -21	-	expression tag
Asp -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Lys -17	-	expression tag
Leu -16	-	expression tag
Thr -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Lys -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

J, I: 1 - 409 (UniProt: Q8TB45)

PDB	External Database	Details
Ser 204	Asn 204	variant
Asn 389	Ser 389	variant

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	1051446.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1320.1
All*	Total formula weight	1052766.4

*Water molecules are not included.