7PD0
Functional and structural characterization of redox sensitive superfolder green fluorescent protein and variants
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Green fluorescent protein | polymer | 238 | 26801.2 | 4 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria (Water jellyfish Mesonema victoria) | |
| 2 | E, G, J (A, C) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 3 | Chemie (PGE) | |||
| 3 | F, H, I, K, L... (A, B, C, D) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 7 | Chemie (PEG) | |||
| 4 | O (D) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | P, Q, R, S (A, B, C, D) | water | water | 18.0 | 521 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Val 1 | Met 1 | engineered mutation |
| Arg 30 | Ser 30 | engineered mutation |
| Ser 48 | Cys 48 | engineered mutation |
| Leu 64 | Phe 64 | engineered mutation |
| Cro 66 | Ser 65 | chromophore |
| Cro 66 | Tyr 66 | chromophore |
| Cro 66 | Gly 67 | chromophore |
| Arg 80 | Gln 80 | engineered mutation |
| Ser 99 | Phe 99 | engineered mutation |
| Thr 105 | Asn 105 | engineered mutation |
| Phe 145 | Tyr 145 | engineered mutation |
| Cys 147 | Ser 147 | engineered mutation |
| Thr 153 | Met 153 | engineered mutation |
| Ala 163 | Val 163 | engineered mutation |
| Val 171 | Ile 171 | engineered mutation |
| Cys 204 | Gln 204 | engineered mutation |
| Val 206 | Ala 206 | engineered mutation |
| Arg 223 | Phe 223 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 107204.9 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1285.5 | |
| All* | Total formula weight | 108490.4 |
