7PD0

Functional and structural characterization of redox sensitive superfolder green fluorescent protein and variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9998
Spacegroup name	P 1 21 1
Unit cell lengths	91.850, 51.290, 101.460
Unit cell angles	90.00, 102.28, 90.00

Refinement procedure

Resolution	47.919 - 2.000
R-factor	0.2525
Rwork	0.250
R-free	0.29790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pca
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.920	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	59713	15047
<I/σ(I)>	6.76
Completeness [%]	94.5
Redundancy	2.4
CC(1/2)	0.991	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	30% PEG 4000.