7P75

Re-engineered 2-deoxy-D-ribose-5-phosphate aldolase catalysing asymmetric Michael addition reactions in substrate-free state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, BBB	Deoxyribose-phosphate aldolase	polymer	267	28683.7	2	UniProt (B1IS38) Pfam (PF01791) UniProt (by SIFTS) (P0A6L0) In PDB	Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks)	DERA,2-deoxy-D-ribose 5-phosphate aldolase,Phosphodeoxyriboaldolase,Deoxyriboaldolase
2		water	water		18.0	513	Chemie (HOH)

Sequence modifications

AAA, BBB: 1 - 259 (UniProt: B1IS38)

PDB	External Database	Details
Ser 18	Thr 18	engineered mutation
Gly 22	Asp 22	engineered mutation
Tyr 24	Asp 24	engineered mutation
Ser 47	Cys 47	engineered mutation
Ser 52	Phe 52	engineered mutation
Ser 142	Thr 142	engineered mutation
Leu 172	Lys 172	engineered mutation
Ser 197	Thr 197	engineered mutation
Val 202	Pro 202	engineered mutation
Thr 203	Ala 203	engineered mutation
Ala 206	Val 206	engineered mutation
Gly 239	Ser 239	engineered mutation
Leu 260	-	expression tag
Glu 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57367.5
All*	Total formula weight	57367.5

*Water molecules are not included.