7P2L

thermostabilised 7TM domain of human mGlu5 receptor bound to photoswitchable ligand alloswitch-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Metabotropic glutamate receptor 5,Endolysin,Metabotropic glutamate receptor 5	polymer	464	51918.1	1	UniProt (P41594) UniProt (P00720) Pfam (PF00003) Pfam (PF00959) In PDB	Homo sapiens (Human)	mGluR5,Lysis protein,Lysozyme,Muramidase,mGluR5
2	A	2-chloranyl-~{N}-[2-methoxy-4-[(~{E})-pyridin-2-yldiazenyl]phenyl]benzamide	non-polymer		366.8	1	Chemie (4YI)
3		water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 569 - 678 (UniProt: P41594)

PDB	External Database	Details
Met 546	-	initiating methionine
Val 547	-	expression tag
Ser 548	-	expression tag
Ala 549	-	expression tag
Ile 550	-	expression tag
Val 551	-	expression tag
Leu 552	-	expression tag
Tyr 553	-	expression tag
Val 554	-	expression tag
Leu 555	-	expression tag
Leu 556	-	expression tag
Ala 557	-	expression tag
Ala 558	-	expression tag
Ala 559	-	expression tag
Ala 560	-	expression tag
His 561	-	expression tag
Ser 562	-	expression tag
Ala 563	-	expression tag
Phe 564	-	expression tag
Ala 565	-	expression tag
Ala 566	-	expression tag
Ala 567	-	expression tag
Ser 568	-	expression tag
Ala 579	Glu 579	engineered mutation
Tyr 667	Asn 667	engineered mutation
Ala 669	Ile 669	engineered mutation
Met 675	Gly 675	engineered mutation

A: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1012	Arg 12	engineered mutation
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	engineered mutation

A: 679 - 836 (UniProt: P41594)

PDB	External Database	Details
Ala 742	Thr 742	engineered mutation
Ala 753	Ser 753	engineered mutation
Ala 837	-	expression tag
Ala 838	-	expression tag
Ala 839	-	expression tag
His 840	-	expression tag
His 841	-	expression tag
His 842	-	expression tag
His 843	-	expression tag
His 844	-	expression tag
His 845	-	expression tag
His 846	-	expression tag
His 847	-	expression tag
His 848	-	expression tag
His 849	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51918.1
Non-Polymers*	Number of molecules	1
	Total formula weight	366.8
All*	Total formula weight	52284.9

*Water molecules are not included.