7P2D

Structure of alphaMbeta2/Cd11bCD18 headpiece in complex with a nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform 2 of Integrin alpha-M	polymer	763	84010.9	1	UniProt (P11215) Pfam (PF00092) Pfam (PF01839) Pfam (PF08441) In PDB	Homo sapiens (Human)	CD11 antigen-like family member B,CR-3 alpha chain,Cell surface glycoprotein MAC-1 subunit alpha,Leukocyte adhesion receptor MO1,Neutrophil adherence receptor
2	B	Integrin beta	polymer	473	52692.6	1	UniProt (A0A494C0X7) Pfam (PF17205) Pfam (PF00362) UniProt (by SIFTS) (P05107) In PDB	Homo sapiens (Human)
3	C	hCD11bNb1 nanobody	polymer	129	14127.8	1		Lama glama (Llama)
4	D, E, F, G	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
5	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A: 1 - 756 (UniProt: P11215)

PDB	External Database	Details
Arg 224	Asn 240	conflict
Arg 681	Asn 697	conflict
Thr 755	Cys 771	conflict
Gly 757	-	expression tag
Leu 758	-	expression tag
Glu 759	-	expression tag
Val 760	-	expression tag
Leu 761	-	expression tag
Phe 762	-	expression tag
Gln 763	-	expression tag

B: 1 - 460 (UniProt: A0A494C0X7)

PDB	External Database	Details
Lys 461	-	expression tag
Asn 462	-	expression tag
Cys 463	-	expression tag
Glu 464	-	expression tag
Pro 465	-	expression tag
Ala 466	-	expression tag
Ala 467	-	expression tag
Leu 468	-	expression tag
Gln 469	-	expression tag
Thr 470	-	expression tag
Leu 471	-	expression tag
Phe 472	-	expression tag
Gln 473	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	150831.3
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	3
	Total formula weight	301.4
All*	Total formula weight	152830.3

*Water molecules are not included.