7OZW
Cryo-EM structure of HIV-1 reverse transcriptase with a DNA aptamer in complex with fragment 166 at the transient P-pocket
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Reverse transcriptase/ribonuclease H | polymer | 556 | 64047.4 | 1 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) | Exoribonuclease H,p66 RT |
| 2 | B (B) | Reverse transcriptase/ribonuclease H | polymer | 428 | 50039.5 | 1 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) | Human immunodeficiency virus type 1 BH10 (HIV-1) | Exoribonuclease H,p51 RT |
| 3 | C (F) | DNA (37-MER) | polymer | 37 | 11434.3 | 1 | synthetic construct | ||
| 4 | D (A) | (1~{R},2~{R})-2-phenyl-~{N}-(1,3-thiazol-2-yl)cyclopropane-1-carboxamide | non-polymer | 244.3 | 1 | Chemie (3IR) |
Sequence modifications
A: 1 - 554 (UniProt: P03366)
B: 1 - 428 (UniProt: P03366)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Val 0 | - | expression tag |
| Ser 280 | Cys 879 | engineered mutation |
| Asn 498 | Asp 1097 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 280 | Cys 879 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 125521.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 244.3 | |
| All* | Total formula weight | 125765.5 |
