7OYX

E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S F88Y S247D in complex with spermidine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Putrescine-binding periplasmic protein PotF	polymer	352	39498.6	2	UniProt (P31133) Pfam (PF13416) In PDB	Escherichia coli (strain K12)
2	A, B	SPERMIDINE	non-polymer		145.2	2	Chemie (SPD)
3	A, B	(2~{S})-1-methoxypropan-2-amine	non-polymer		89.1	5	Chemie (3IE)
4	A	(2~{S})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine	non-polymer		191.3	1	Chemie (4SW)
5	A, B	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
6	B	(2~{R})-1-(2-methoxyethoxy)propan-2-amine	non-polymer		133.2	1	Chemie (4RH)
7	B	(2~{R})-1-[(2~{R})-1-[(2~{S})-1-[(2~{S})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-yl]oxypropan-2-amine	non-polymer		307.4	1	Chemie (3IK)
8	B	(2~{S})-1-(2-methoxyethoxy)propan-2-amine	non-polymer		133.2	1	Chemie (ONT)
9		water	water		18.0	766	Chemie (HOH)

Sequence modifications

A, B: 27 - 370 (UniProt: P31133)

PDB	External Database	Details
Thr 38	Ser 38	engineered mutation
Tyr 88	Phe 88	engineered mutation
Asp 182	Ala 182	engineered mutation
Trp 276	Phe 276	engineered mutation
Gln 348	Leu 348	engineered mutation
Leu 371	-	expression tag
Glu 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78997.1
Non-Polymers*	Number of molecules	14
	Total formula weight	1607.6
All*	Total formula weight	80604.8

*Water molecules are not included.