7OYX
E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S F88Y S247D in complex with spermidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 117.155, 71.493, 92.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.700 - 1.370 |
R-factor | 0.1657 |
Rwork | 0.165 |
R-free | 0.19330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a99 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.453 |
Data reduction software | XDS (20190806) |
Data scaling software | XSCALE (20190806) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.700 | 1.410 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.154 | 3.290 |
Rmeas | 0.166 | 3.540 |
Rpim | 0.061 | 1.295 |
Number of reflections | 163742 | 16029 |
<I/σ(I)> | 7.21 | 0.46 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 7.4 | 7.3 |
CC(1/2) | 0.997 | 0.240 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 2.4 M AmSO4 + 0.1 M Bicine pH 8.3 + 4.5% Jeffamine M600 |