7OYV

E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88A S247D in complex with spermidine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putrescine-binding periplasmic protein	polymer	352	39482.6	2	UniProt (A0A6S5NLK9) Pfam (PF13416) UniProt (by SIFTS) (P31133)	Escherichia coli
2	C (A)	(2~{R})-1-[(2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-amine	non-polymer		249.3	1	Chemie (3IZ)
3	D, E, J, K, L (A, B)	(2R)-1-methoxypropan-2-amine	non-polymer		89.1	5	Chemie (JFN)
4	F, M (A, B)	SPERMIDINE	non-polymer		145.2	2	Chemie (SPD)
5	G, H, N (A, B)	(2~{S})-1-methoxypropan-2-amine	non-polymer		89.1	3	Chemie (3IE)
6	I, O (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	P, Q (A, B)	water	water		18.0	224	Chemie (HOH)

Sequence modifications

A, B: 27 - 370 (UniProt: A0A6S5NLK9)

PDB	External Database	Details
Thr 38	Ser 21	engineered mutation
Tyr 87	Ser 70	engineered mutation
Ala 88	Phe 71	engineered mutation
Asp 182	Ala 165	engineered mutation
Trp 276	Phe 259	engineered mutation
Gln 348	Leu 331	engineered mutation
Leu 371	-	expression tag
Glu 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78965.1
Non-Polymers*	Number of molecules	13
	Total formula weight	1323.8
All*	Total formula weight	80289.0

*Water molecules are not included.