7OYV
E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88A S247D in complex with spermidine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Putrescine-binding periplasmic protein | polymer | 352 | 39482.6 | 2 | UniProt (A0A6S5NLK9) Pfam (PF13416) UniProt (by SIFTS) (P31133) In PDB | Escherichia coli | |
2 | A | (2~{R})-1-[(2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-amine | non-polymer | 249.3 | 1 | Chemie (3IZ) | |||
3 | A, B | (2R)-1-methoxypropan-2-amine | non-polymer | 89.1 | 5 | Chemie (JFN) | |||
4 | A, B | SPERMIDINE | non-polymer | 145.2 | 2 | Chemie (SPD) | |||
5 | A, B | (2~{S})-1-methoxypropan-2-amine | non-polymer | 89.1 | 3 | Chemie (3IE) | |||
6 | A, B | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
7 | water | water | 18.0 | 224 | Chemie (HOH) |
Sequence modifications
A, B: 27 - 370 (UniProt: A0A6S5NLK9)
PDB | External Database | Details |
---|---|---|
Thr 38 | Ser 21 | engineered mutation |
Tyr 87 | Ser 70 | engineered mutation |
Ala 88 | Phe 71 | engineered mutation |
Asp 182 | Ala 165 | engineered mutation |
Trp 276 | Phe 259 | engineered mutation |
Gln 348 | Leu 331 | engineered mutation |
Leu 371 | - | expression tag |
Glu 372 | - | expression tag |
His 373 | - | expression tag |
His 374 | - | expression tag |
His 375 | - | expression tag |
His 376 | - | expression tag |
His 377 | - | expression tag |
His 378 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 78965.1 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 1323.8 | |
All* | Total formula weight | 80289.0 |