7OYV
E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88A S247D in complex with spermidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.613, 122.576, 190.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.580 - 1.900 |
R-factor | 0.2289 |
Rwork | 0.227 |
R-free | 0.27630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a99 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.863 |
Data reduction software | XDS (20190806) |
Data scaling software | XSCALE (20190806) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.580 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.121 | 2.003 |
Rmeas | 0.128 | 2.139 |
Rpim | 0.039 | 0.712 |
Number of reflections | 62244 | 5363 |
<I/σ(I)> | 13.74 | 1 |
Completeness [%] | 90.9 | 79.5 |
Redundancy | 9.5 | 7.6 |
CC(1/2) | 0.998 | 0.375 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 2.4 M AmSO4 + 0.1 M Bicine pH 8.3 + 4.5% Jeffamine M600 |