7OYV
E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88A S247D in complex with spermidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.613, 122.576, 190.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.580 - 1.900 |
| R-factor | 0.2289 |
| Rwork | 0.227 |
| R-free | 0.27630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a99 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS (20190806) |
| Data scaling software | XSCALE (20190806) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.121 | 2.003 |
| Rmeas | 0.128 | 2.139 |
| Rpim | 0.039 | 0.712 |
| Number of reflections | 62244 | 5363 |
| <I/σ(I)> | 13.74 | 1 |
| Completeness [%] | 90.9 | 79.5 |
| Redundancy | 9.5 | 7.6 |
| CC(1/2) | 0.998 | 0.375 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 2.4 M AmSO4 + 0.1 M Bicine pH 8.3 + 4.5% Jeffamine M600 |
