7OS2

Cryo-EM structure of Brr2 in complex with Jab1/MPN and C9ORF78

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	U5 small nuclear ribonucleoprotein 200 kDa helicase	polymer	1739	198785.8	1	UniProt (O75643) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) In PDB	Homo sapiens (Human)
2	C	Telomere length and silencing protein 1 homolog	polymer	293	34081.5	1	UniProt (Q9NZ63) Pfam (PF07052) In PDB	Homo sapiens (Human)
3	J	Pre-mRNA-processing-splicing factor 8	polymer	278	31861.0	1	UniProt (Q6P2Q9) Pfam (PF01398) Pfam (PF08084) In PDB	Homo sapiens (Human)

Sequence modifications

B: 394 - 2129 (UniProt: O75643)

PDB	External Database	Details
Gly 391	-	expression tag
Ala 392	-	expression tag
Glu 393	-	expression tag
Phe 394	Met 394	expression tag

C: 1 - 289 (UniProt: Q9NZ63)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

J: 2064 - 2335 (UniProt: Q6P2Q9)

PDB	External Database	Details
Gly 2058	-	expression tag
Pro 2059	-	expression tag
Leu 2060	-	expression tag
Gly 2061	-	expression tag
Ser 2062	-	expression tag
Met 2063	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	264728.3
All*	Total formula weight	264728.3

*Water molecules are not included.