7OPL

CryoEM structure of DNA Polymerase alpha - primase bound to SARS CoV nsp1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase alpha catalytic subunit	polymer	1170	133702.6	1	UniProt (P09884) Pfam (PF03104) Pfam (PF00136) Pfam (PF08996)	Homo sapiens (Human)	DNA polymerase alpha catalytic subunit p180
2	B (B)	DNA polymerase alpha subunit B	polymer	452	49855.4	1	UniProt (Q14181) Pfam (PF22062) Pfam (PF04042)	Homo sapiens (Human)	DNA polymerase alpha 70 kDa subunit
3	C (C)	DNA primase small subunit	polymer	441	52590.8	1	UniProt (P49642) Pfam (PF01896)	Homo sapiens (Human)	DNA primase 49 kDa subunit,p49
4	D (D)	DNA primase large subunit	polymer	509	58890.9	1	UniProt (P49643) Pfam (PF04104)	Homo sapiens (Human)	DNA primase 58 kDa subunit,p58
5	E (E)	Non-structural protein 1	polymer	119	12955.9	1	UniProt (P0C6U8) Pfam (PF11501)	Severe acute respiratory syndrome coronavirus (SARS-CoV)	nsp1,Leader protein
6	F, G, H (A, C)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
7	I (D)	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 334 - 1462 (UniProt: P09884)

PDB	External Database	Details
Met 293	-	initiating methionine
Ser 294	-	expression tag
Ala 295	-	expression tag
Trp 296	-	expression tag
Ser 297	-	expression tag
His 298	-	expression tag
Pro 299	-	expression tag
Gln 300	-	expression tag
Phe 301	-	expression tag
Glu 302	-	expression tag
Lys 303	-	expression tag
Gly 304	-	expression tag
Gly 305	-	expression tag
Gly 306	-	expression tag
Ser 307	-	expression tag
Gly 308	-	expression tag
Gly 309	-	expression tag
Gly 310	-	expression tag
Ser 311	-	expression tag
Gly 312	-	expression tag
Gly 313	-	expression tag
Gly 314	-	expression tag
Ser 315	-	expression tag
Trp 316	-	expression tag
Ser 317	-	expression tag
His 318	-	expression tag
Pro 319	-	expression tag
Gln 320	-	expression tag
Phe 321	-	expression tag
Glu 322	-	expression tag
Lys 323	-	expression tag
Leu 324	-	expression tag
Glu 325	-	expression tag
Val 326	-	expression tag
Leu 327	-	expression tag
Phe 328	-	expression tag
Gln 329	-	expression tag
Gly 330	-	expression tag
Pro 331	-	expression tag
Glu 332	-	expression tag
Phe 333	-	expression tag

B: 149 - 598 (UniProt: Q14181)

PDB	External Database	Details
Met 147	-	initiating methionine
Gly 148	-	expression tag

C: 1 - 420 (UniProt: P49642)

PDB	External Database	Details
Met -20	-	initiating methionine
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Thr -1	-	expression tag
Ser 0	-	expression tag

E: 13 - 127 (UniProt: P0C6U8)

PDB	External Database	Details
Gly 9	-	expression tag
Ser 10	-	expression tag
Met 11	-	expression tag
Gly 12	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	307995.6
Non-Polymers*	Number of molecules	4
	Total formula weight	547.9
All*	Total formula weight	308543.4

*Water molecules are not included.