7OMR

Crystal structure of coelenteramide-bound Renilla reniformis luciferase RLuc8-D162A variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Coelenterazine h 2-monooxygenase	polymer	317	36903.2	1	UniProt (P27652) Pfam (PF00561) In PDB	Renilla reniformis (Sea pansy)	Renilla-luciferin 2-monooxygenase,Renilla-type luciferase
2	A	N-[3-BENZYL-5-(4-HYDROXYPHENYL)PYRAZIN-2-YL]-2-(4-HYDROXYPHENYL)ACETAMIDE	non-polymer		411.5	1	Chemie (CEI)
3	A	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4		water	water		18.0	343	Chemie (HOH)

Sequence modifications

A: 1 - 311 (UniProt: P27652)

PDB	External Database	Details
Thr 55	Ala 55	conflict
Ala 124	Cys 124	conflict
Ala 130	Ser 130	conflict
Arg 136	Lys 136	conflict
Met 143	Ala 143	conflict
Ala 162	Asp 162	engineered mutation
Val 185	Met 185	conflict
Leu 253	Met 253	conflict
Leu 287	Ser 287	conflict
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36903.2
Non-Polymers*	Number of molecules	5
	Total formula weight	779.8
All*	Total formula weight	37683.0

*Water molecules are not included.