7OMR
Crystal structure of coelenteramide-bound Renilla reniformis luciferase RLuc8-D162A variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Coelenterazine h 2-monooxygenase | polymer | 317 | 36903.2 | 1 | UniProt (P27652) Pfam (PF00561) | Renilla reniformis (Sea pansy) | Renilla-luciferin 2-monooxygenase,Renilla-type luciferase |
| 2 | B (A) | N-[3-BENZYL-5-(4-HYDROXYPHENYL)PYRAZIN-2-YL]-2-(4-HYDROXYPHENYL)ACETAMIDE | non-polymer | 411.5 | 1 | Chemie (CEI) | |||
| 3 | C, D, E, F (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | G (A) | water | water | 18.0 | 343 | Chemie (HOH) |
Sequence modifications
A: 1 - 311 (UniProt: P27652)
| PDB | External Database | Details |
|---|---|---|
| Thr 55 | Ala 55 | conflict |
| Ala 124 | Cys 124 | conflict |
| Ala 130 | Ser 130 | conflict |
| Arg 136 | Lys 136 | conflict |
| Met 143 | Ala 143 | conflict |
| Ala 162 | Asp 162 | engineered mutation |
| Val 185 | Met 185 | conflict |
| Leu 253 | Met 253 | conflict |
| Leu 287 | Ser 287 | conflict |
| His 312 | - | expression tag |
| His 313 | - | expression tag |
| His 314 | - | expression tag |
| His 315 | - | expression tag |
| His 316 | - | expression tag |
| His 317 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 36903.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 779.8 | |
| All* | Total formula weight | 37683.0 |
