7OMD
Crystal structure of azacoelenterazine-bound Renilla reniformis luciferase variant RLuc8-D162A
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Coelenterazine h 2-monooxygenase | polymer | 317 | 36903.2 | 2 | UniProt (P27652) Pfam (PF00561) In PDB | Renilla reniformis (Sea pansy) | Renilla-luciferin 2-monooxygenase,Renilla-type luciferase |
2 | A, B | 6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-[1,2,4]triazolo[4,3-a]pyrazin-3-one | non-polymer | 424.5 | 4 | Chemie (VK8) | |||
3 | A | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 1 | Chemie (DMS) | |||
4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
5 | B | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
6 | water | water | 18.0 | 690 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 311 (UniProt: P27652)
PDB | External Database | Details |
---|---|---|
Thr 55 | Ala 55 | conflict |
Ala 124 | Cys 124 | conflict |
Ala 130 | Ser 130 | conflict |
Arg 136 | Lys 136 | conflict |
Met 143 | Ala 143 | conflict |
Ala 162 | Asp 162 | engineered mutation |
Val 185 | Met 185 | conflict |
Leu 253 | Met 253 | conflict |
Leu 287 | Ser 287 | conflict |
His 312 | - | expression tag |
His 313 | - | expression tag |
His 314 | - | expression tag |
His 315 | - | expression tag |
His 316 | - | expression tag |
His 317 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 73806.3 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 1917.5 | |
All* | Total formula weight | 75723.8 |