7OK0
Cryo-EM structure of the Sulfolobus acidocaldarius RNA polymerase at 2.88 A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA-directed RNA polymerase subunit A' | polymer | 880 | 99929.1 | 1 | UniProt (P11512) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO1 |
| 2 | B | DNA-directed RNA polymerase subunit B | polymer | 1126 | 126647.1 | 1 | UniProt (P11513) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO2 |
| 3 | C | DNA-directed RNA polymerase subunit A'' | polymer | 393 | 44507.4 | 1 | UniProt (P11514) Pfam (PF04998) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO1 |
| 4 | D | DNA-directed RNA polymerase subunit D | polymer | 264 | 29858.8 | 1 | UniProt (P39471) Pfam (PF01193) Pfam (PF01000) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO3 |
| 5 | E | DNA-directed RNA polymerase subunit E | polymer | 183 | 20496.8 | 1 | UniProt (P39466) Pfam (PF03876) Pfam (PF00575) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO7 |
| 6 | F | DNA-directed RNA polymerase, subunit F | polymer | 114 | 13071.0 | 1 | UniProt (Q4JB12) Pfam (PF03874) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO4 |
| 7 | G | DNA-directed RNA polymerase, subunit G | polymer | 130 | 15292.8 | 1 | UniProt (Q4JAY4) Pfam (PF16992) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO8 |
| 8 | H | DNA-directed RNA polymerase subunit H | polymer | 84 | 9477.1 | 1 | UniProt (P11521) Pfam (PF01191) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO5 |
| 9 | K | DNA-directed RNA polymerase subunit K | polymer | 89 | 10252.0 | 1 | UniProt (P39463) Pfam (PF01192) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO6 |
| 10 | L | DNA-directed RNA polymerase subunit L | polymer | 90 | 10061.8 | 1 | UniProt (P46217) Pfam (PF13656) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO11 |
| 11 | N | DNA-directed RNA polymerase subunit N | polymer | 66 | 7673.1 | 1 | UniProt (P39472) Pfam (PF01194) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO10 |
| 12 | P | DNA-directed RNA polymerase subunit P | polymer | 48 | 5662.9 | 1 | UniProt (Q4JAE8) Pfam (PF03604) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit RPO12 |
| 13 | Q | Conserved protein | polymer | 105 | 12331.7 | 1 | UniProt (Q4JAJ6) Pfam (PF12136) In PDB | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) | DNA-directed RNA polymerase subunit 13 |
| 14 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 15 | A, B, N, P | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 16 | D | FE3-S4 CLUSTER | non-polymer | 295.8 | 1 | Chemie (F3S) |
Sequence modifications
B: 1 - 1126 (UniProt: P11513)
G: 1 - 124 (UniProt: Q4JAY4)
| PDB | External Database | Details |
|---|---|---|
| Ile 772 | Val 772 | variant |
| PDB | External Database | Details |
|---|---|---|
| His 125 | - | expression tag |
| His 126 | - | expression tag |
| His 127 | - | expression tag |
| His 128 | - | expression tag |
| His 129 | - | expression tag |
| His 130 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 405261.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 712.6 | |
| All* | Total formula weight | 405973.9 |
