7OD9

Crystal structure of activated CheY fused to the C-terminal domain of CheF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Response regulator receiver protein	polymer	142	15673.3	2	UniProt (G0H061) Pfam (PF00072)	Methanococcus maripaludis X1	CheY from Methanococcus maripaludis
2	C, D (C, F)	C-terminal domain of CheF from Methanococcus maripaludis	polymer	115	12632.3	2	UniProt (G0H062)	Methanococcus maripaludis X1
3	E, G (B, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, H (B, A)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	2	Chemie (BEF)
5	I, J, K, L (B, A, C, F)	water	water		18.0	83	Chemie (HOH)

Sequence modifications

B, A: 3 - 123 (UniProt: G0H061)

PDB	External Database	Details
Met -5	-	initiating methionine
Gly -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
Val 124	-	expression tag
Leu 125	-	expression tag
Phe 126	-	expression tag
Gln 127	-	expression tag
Gly 128	-	expression tag
Pro 129	-	expression tag
Ser 130	-	expression tag
Ala 131	-	expression tag
Gly 132	-	expression tag
Leu 133	-	expression tag
Val 134	-	expression tag
Pro 135	-	expression tag
Arg 136	-	expression tag

C, F: 245 - 348 (UniProt: G0H062)

PDB	External Database	Details
Gly 234	-	expression tag
Ser 235	-	expression tag
Gly 236	-	expression tag
Gly 237	-	expression tag
Ile 238	-	expression tag
Glu 239	-	expression tag
Gly 240	-	expression tag
Gly 241	-	expression tag
Ser 242	-	expression tag
Met 243	-	expression tag
Gly 244	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	56611.0
Non-Polymers*	Number of molecules	4
	Total formula weight	180.6
All*	Total formula weight	56791.7

*Water molecules are not included.