7OD9
Crystal structure of activated CheY fused to the C-terminal domain of CheF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.290, 70.870, 59.820 |
Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
Resolution | 44.070 - 2.300 |
R-factor | 0.2524 |
Rwork | 0.249 |
R-free | 0.30660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ekh |
RMSD bond length | 0.005 |
RMSD bond angle | 0.864 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.070 | 2.383 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 20987 | 2097 |
<I/σ(I)> | 7.66 | 0.88 |
Completeness [%] | 99.6 | |
Redundancy | 6.5 | |
CC(1/2) | 0.997 | 0.577 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 MgCl2, 0.1 M Tris pH 8.5, 20 % (w/v) PEG 8000 |