7OBU
Crystal structure of holo-F210W mutant of Hydroxy ketone aldolase (SwHKA) from Sphingomonas wittichii RW1, with the active site in the resting and the active state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | HpcH/HpaI aldolase | polymer | 252 | 26823.6 | 2 | UniProt (A5VH82) UniProt (by SIFTS) (A0AAT8XT80) | Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) | |
| 2 | C, F (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D, G (A, B) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 4 | E (B) | 3-HYDROXYPYRUVIC ACID | non-polymer | 104.1 | 1 | Chemie (3PY) | |||
| 5 | H, I (A, B) | water | water | 18.0 | 619 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 251 (UniProt: A5VH82)
| PDB | External Database | Details |
|---|---|---|
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Trp 210 | Phe 210 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 53647.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 230.9 | |
| All* | Total formula weight | 53878.0 |
