7OBU
Crystal structure of holo-F210W mutant of Hydroxy ketone aldolase (SwHKA) from Sphingomonas wittichii RW1, with the active site in the resting and the active state
Functional Information from GO Data
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"Q47098","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"36214476","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"2019","firstPage":"2649","lastPage":"2649","volume":"361","journal":"Adv. Synth. Catal.","title":"CH-pi Interactions Promote the Conversion of Hydroxypyruvate in a Class II Pyruvate Aldolase.","authors":["Marsden S.R.","Mestrom L.","Bento I.","Hagedoorn P.L.","McMillan D.G.G.","Hanefeld U."],"citationCrossReferences":[{"database":"DOI","id":"10.1002/adsc.201900205"}]}},{"source":"PDB","id":"6R62","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7NNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7O5V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8ADQ","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"36214476","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"2019","firstPage":"2649","lastPage":"2649","volume":"361","journal":"Adv. Synth. Catal.","title":"CH-pi Interactions Promote the Conversion of Hydroxypyruvate in a Class II Pyruvate Aldolase.","authors":["Marsden S.R.","Mestrom L.","Bento I.","Hagedoorn P.L.","McMillan D.G.G.","Hanefeld U."],"citationCrossReferences":[{"database":"DOI","id":"10.1002/adsc.201900205"}]}},{"source":"PDB","id":"6R62","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7NNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7NR1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7NUJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8ADQ","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"source":"UniProtKB","id":"Q47098","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Site: {"description":"Increases basicity of active site His","evidences":[{"source":"UniProtKB","id":"Q47098","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Improves activity by CH-pi interactions","evidences":[{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"journal article","publicationDate":"2019","firstPage":"2649","lastPage":"2649","volume":"361","journal":"Adv. Synth. Catal.","title":"CH-pi Interactions Promote the Conversion of Hydroxypyruvate in a Class II Pyruvate Aldolase.","authors":["Marsden S.R.","Mestrom L.","Bento I.","Hagedoorn P.L.","McMillan D.G.G.","Hanefeld U."],"citationCrossReferences":[{"database":"DOI","id":"10.1002/adsc.201900205"}]}}]} |
| Chain | Residue | Details |
