7O7C

Crystal structure of rsEGFP2 mutant V151A in the non-fluorescent off-state determined by synchrotron radiation at 100K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	252	28504.1	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria
2	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3		water	water		18.0	264	Chemie (HOH)

Sequence modifications

A: 1 - 239 (UniProt: P42212)

PDB	External Database	Details
Met -12	-	initiating methionine
Arg -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Thr -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Val 2	-	insertion
Leu 65	Phe 64	engineered mutation
Pia 68	Ser 65	chromophore
Pia 68	Tyr 66	chromophore
Pia 68	Gly 67	chromophore
Leu 70	Gln 69	engineered mutation
Ala 151	Val 150	engineered mutation
Ser 164	Val 163	engineered mutation
Lys 207	Ala 206	engineered mutation
Leu 232	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28504.1
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	28792.3

*Water molecules are not included.