7O7C

Crystal structure of rsEGFP2 mutant V151A in the non-fluorescent off-state determined by synchrotron radiation at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	51.160, 60.290, 66.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.570 - 1.550
R-factor	0.1656
Rwork	0.164
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dtx
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.570	1.590
High resolution limit [Å]	1.550	1.550
Rmeas	0.045	0.606
Number of reflections	29619	2528
<I/σ(I)>	14.82	2.26
Completeness [%]	97.0
Redundancy	3
CC(1/2)	0.999	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	100 mM HEPES pH 7.8 - 8.4 and 1.7 - 2.4 M ammonium sulfate