7O6H
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with tri-mannuronic acid
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Alginate lyase (PL7) | polymer | 231 | 25452.7 | 1 | UniProt (A0A485PVH1) Pfam (PF08787) In PDB | Paradendryphiella salina | |
2 | B | beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | branched | 546.4 | 1 | In PDB GlyTouCan (G85027VZ) | |||
3 | A | alpha-D-mannopyranuronic acid | non-polymer | 194.1 | 1 | Chemie (MAV) | |||
4 | water | water | 18.0 | 114 | Chemie (HOH) |
Sequence modifications
A: 23 - 243 (UniProt: A0A485PVH1)
PDB | External Database | Details |
---|---|---|
Glu -1 | - | expression tag |
Phe 0 | - | expression tag |
Phe 223 | Tyr 223 | engineered mutation |
Val 244 | - | expression tag |
Asp 245 | - | expression tag |
His 246 | - | expression tag |
His 247 | - | expression tag |
His 248 | - | expression tag |
His 249 | - | expression tag |
His 250 | - | expression tag |
His 251 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 25452.7 | |
Branched | Number of molecules | 1 |
Total formula weight | 546.4 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 194.1 | |
All* | Total formula weight | 26193.2 |